Abstract:
:PCR-based analyses were performed for the identification of HLA-B44 subgroups. Genomic DNA from six homozygous cell lines and 44 healthy individuals who had serologically tested positive for HLA-B44 was investigated for polymorphism in exons 2 and 3 of the HLA-B44 genes. Two primers were designed for specific amplification of the B*4401 allele in exon 2. None of the tested genomic DNAs, including the cell line "BAU-J" from which the sequence of B*4401 was derived, was amplified successfully using these primers, indicating that the B*4401 sequence may not be correct in position 242-244. For identification of the B*4402 and *4403 subtypes we specifically amplified the B44 gene in exon 3 using two sequence-specific primers. The PCR products, which were obtained from all B44-positive samples (n = 50) and from none of the B44-negative controls (n = 20), were subsequently hybridized with the dig-ddUTP-labeled oligonucleotides. The base substitution at position 146, as described previously for B*4401 and *4402 (C for G), could not be confirmed by oligonucleotide hybridization. In contrast, the oligonucleotide typing for G in position 146 gave positive signals in all B44-positive samples. Except for one, HLA-B44-positive DNAs from LCLs and healthy individuals could be divided into two subgroups according to the polymorphic region in position 195-197. Out of 44 unrelated individuals with B44, 27 (61%) were positive for B*4402 and 16 (36%) were positive for B*4403.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Hum Immunoljournal_title
Human immunologyauthors
Yao Z,Keller E,Scholz S,McNicholas A,Volgger A,Albert EDdoi
10.1016/0198-8859(94)00071-wsubject
Has Abstractpub_date
1995-01-01 00:00:00pages
54-60issue
1eissn
0198-8859issn
1879-1166pii
019888599400071Wjournal_volume
42pub_type
杂志文章相关文献
HUMAN IMMUNOLOGY文献大全abstract::MHC class II molecules play a central role in the control of the immune response, but their biologic function and mechanism of action on the surface of activated human T lymphocytes are not entirely understood. In our study, the functional role of HLA class II molecules in T-blast proliferation was investigated by ana...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(93)90552-c
更新日期:1993-12-01 00:00:00
abstract::To elucidate the residues important for the binding of peptides to HLA-Cw3, a substitutional analysis of two HLA-Cw*0304-binding peptides was performed. The optimal registry and length for a Cw3-restricted epitope from HIV-1 p24gag was determined to be a nonamer, p24gag 144-152. Substituted analogs of this nonamer pep...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(98)00053-6
更新日期:1998-08-01 00:00:00
abstract::Heart transplant rejection is routinely defined by histological evaluation of endomyocardial biopsies (EMB). As elevated levels of donor derived sHLA (dsHLA) can be detected in the serum of transplanted patients just before or during rejection, quantification of donor specific soluble counterparts of HLA Class I (sHLA...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(99)00010-5
更新日期:1999-05-01 00:00:00
abstract::Recent data puts WNT signaling pathway in a pivotal role in regulating pancreas development as well as islet function, insulin production and secretion. The key effectors in the WNT signaling pathway are low-density lipoprotein receptor-related protein 5 (LRP5), transcription factor 7-like 2 (TCF7L2), and downstream-r...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2015.03.005
更新日期:2015-05-01 00:00:00
abstract::A two stage PCR-SSOP typing procedure, that permitted HLA-B allele assignment, was applied to DNA samples obtained from six diverse populations -Brazilian, Mexican (Series and Mestizos), Cuban (Caucasoid and Mulatto), South African Zulu, Omani, and Singapore Chinese. HLA-B allele frequencies and HLA-A/B two locus hapl...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(01)00247-6
更新日期:2001-06-01 00:00:00
abstract::Bacterial sensing is crucial for appropriate response by the innate and adaptive immune system against invading microorganisms. Single nucleotide polymorphisms (SNPs) in genes involved in bacterial recognition, CARD15 and TLR4, increased the risk of inflammatory bowel disease (IBD) in a New Zealand Caucasian case-cont...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2009.03.002
更新日期:2009-06-01 00:00:00
abstract::While modern high-throughput sequence-based HLA genotyping methods generally provide highly accurate typing results, artefacts may nonetheless arise for numerous reasons, such as sample contamination, sequencing errors, read misalignments, or PCR amplification biases. To help detecting spurious typing results, we test...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2016.01.012
更新日期:2016-03-01 00:00:00
abstract::Besides central tolerance peripheral tolerance is an important mechanism to avoid development of autoimmunity. Naturally occurring thymic-derived regulatory T cells (nTreg) mediate peripheral tolerance by suppressing autoreactive T cells clones having escaped thymic deletional control. This implies that nTreg have the...
journal_title:Human immunology
pub_type: 杂志文章,评审
doi:10.1016/j.humimm.2015.06.015
更新日期:2015-08-01 00:00:00
abstract::"Those who do not know the past are destined to repeat it". The current system for the allocation of deceased donor kidneys that was implemented in December 2014 (termed the kidney allocation system (KAS)) was the culmination of a decade-long process. Thus, many people involved in transplantation today may not be awar...
journal_title:Human immunology
pub_type: 历史文章,杂志文章
doi:10.1016/j.humimm.2016.08.008
更新日期:2017-01-01 00:00:00
abstract::Mononuclear cells obtained from a child at the acute presentation of type I diabetes were stimulated in vitro with human insulin followed by IL-2 and IL-4. All of the T-cell clones isolated from this stimulation were autoreactive, recognizing autologous B cells in the absence of insulin or other exogenous antigens. El...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(93)90128-n
更新日期:1993-04-01 00:00:00
abstract:BACKGROUND:Leucocyte infiltration and activation in the central nervous system (CNS) is an important step in the pathogenesis of multiple sclerosis (MS). The Chemokine receptor 5 (CCR5) is implicated in immune cell migration and cytokine release in the CNS, and it was demonstrated to strongly contribute to CNS inflamma...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2018.04.015
更新日期:2018-08-01 00:00:00
abstract::Using the soft-agar colony assay, we have generated three MT3-associated clones: HJ1, HJ13, and HJ39, from an MLR combination of two unrelated individuals. Another clone, HJ37, appeared to recognize a novel HLA-D determinant. PLT inhibition studies with monoclonal anti-Ia-like antibodies (Mab) were conducted on clones...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(83)90066-6
更新日期:1983-02-01 00:00:00
abstract::To examine the genetic diversity in Morocco, the polymorphism at the HLA-DRB1 locus was investigated in two populations: the Metalsa group consisting of Berbers from north Morocco (who speak the Tarifit language and live in the Nador area), and the Chaouya group who are Arabic-speaking people from west Morocco (Atlant...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(01)00370-6
更新日期:2002-02-01 00:00:00
abstract::The monoclonal antibodies (MoAbs) KS3 and KS4 are secreted by hybridomas constructed with splenocytes from a BALB/c mouse sequentially immunized with the cultured lymphoid cells JKu and LG-2 which share only the HLA-B27 specificity. Serologic and immunochemical assays have shown that the two MoAbs recognize the same (...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(88)90071-7
更新日期:1988-03-01 00:00:00
abstract::HLA-G molecules seem to have a protective effect for the semi-allogeneic fetus by mother immunosuppression. Also, pregnancy pathologies have been associated to HLA-G(∗)01:05N "null allele". In addition, other general regulatory immune functions have been associated to HLA-G in infections, tumors and autoimmunity. Thus...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2012.12.013
更新日期:2013-04-01 00:00:00
abstract::A comprehensive system for genetic typing of the HLA class I A locus is described, based on PCR amplification and typing with nonradioactively labeled SSO probes. Exons 1-3 of the A locus are amplified and typing is performed with a set of 30 nonradioactively labeled oligonucleotide probes. This system resolves 34 of ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(94)90018-3
更新日期:1994-05-01 00:00:00
abstract::Antigen-selected T cell receptor (TCR) repertoires vary in complexity from very limited to extremely diverse. We have previously characterized two different CD8 T cell responses, which are restricted by the same mouse major histocompatibility complex (MHC) class I molecule, H-2 Kd. The TCR repertoire in the response a...
journal_title:Human immunology
pub_type: 杂志文章,评审
doi:10.1016/s0198-8859(97)00082-7
更新日期:1997-05-01 00:00:00
abstract::This study presents a comparative analysis of the effects of different interferons (IFN) on the three recognizable subsets of human HLA class II molecules: DR, BR, and DQ. Both cellular expression and shedding of class II molecules have been determined on three different cell types. The results can be summarized as fo...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(85)90022-9
更新日期:1985-05-01 00:00:00
abstract::T lymphocyte clones (TLCs) specific for Chlamydia trachomatis were obtained after limiting dilution of activated T cells from a single donor, BS (HLA-A3; B7,15; DR1, 4; DRw53; DPw4). Most of the proliferative TLCs obtained apparently used restriction elements on DR1 or DR4 molecules, expressed in the antigen-presentin...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(84)90060-0
更新日期:1984-12-01 00:00:00
abstract::Interleukin (IL)-1 gene polymorphisms affect several inflammatory diseases, including cancer. Therefore, we studied genetic association of biallelic (-511C>T) polymorphism of IL-1 beta and 86-bp VNTR polymorphism of IL-1RN in 159 patients with esophageal cancer (EC) and 194 age- and gender-matched healthy controls. Ge...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2008.01.012
更新日期:2008-03-01 00:00:00
abstract::Most sialic acid-binding immunoglobulin-like lectins (Siglecs) suppress immune cell function but are expressed at low levels on human T cells. We found that soluble CD52 inhibited T cell signalling by ligating Siglec-10, but the presence of Siglec-10 on human T cells has been questioned. To address this concern, we ex...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2020.01.009
更新日期:2020-01-01 00:00:00
abstract::We have studied HLA-DQ encoded antigens from HLA-DRw6 homozygous cells and analyzed the DQ region at the DNA level. HLA-DQ molecules were isolated from EBV transformed B-cell lines and analyzed for DQ alpha and DQ beta polymorphism. From the same set of cells, DNA was isolated and analyzed for RFLP. Polymorphism could...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(87)90097-8
更新日期:1987-06-01 00:00:00
abstract::We studied HLA class I (HLA-A, -B) and class II (HLA-DRB1, -DQB1) alleles by PCR-SSP based typing in 250 Mexicans from the states of Baja California Norte and Baja California Sur living in Mexicali (N = 100), La Paz (N = 75), Tijuana (N = 25) and rural communities (N = 50) to obtain information regarding allelic and h...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2019.06.007
更新日期:2020-09-01 00:00:00
abstract::The clonal selection theory of immune reactivity is based on a metaphor of self/not-self discrimination and considers self-tolerance to result from clonal deletion. There is evidence that a deletional mechanism is responsible for negative selection of self MHC-reactive T-cells in the thymus. Bretscher/Cohn theory buil...
journal_title:Human immunology
pub_type: 历史文章,杂志文章,评审
doi:10.1016/S0198-8859(96)00294-7
更新日期:1997-02-01 00:00:00
abstract::There is increased evidence about the deleterious effect of delayed graft function (DGF) in both short- and long-term kidney graft outcome. Among the mechanisms involved in the production of DGF, immune factors play a role, especially in the level of hypersensitization. From the 1389 patients transplanted at our hospi...
journal_title:Human immunology
pub_type: 杂志文章,评审
doi:10.1016/j.humimm.2005.01.026
更新日期:2005-04-01 00:00:00
abstract::The dendritic cell (DC) lineage encompasses a diverse population of cells with unique subtype-specific functions. In peripheral blood, four DC subsets have been identified based on their distinct expression of CD1c, CD141, CD16, and CD123, and these subpopulations exhibit functional properties in immune responses. How...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2010.07.008
更新日期:2010-10-01 00:00:00
abstract::In our earlier studies, single nucleotide polymorphisms (SNPs) associated with anti-inflammatory cytokines were found to influence risk for breast cancer in western Indian women. Analysis of Interleukin 6 (IL-6) -174G>C polymorphism in this cohort (patients = 182; controls = 236) suggested a protective role for IL-6 -...
journal_title:Human immunology
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.humimm.2014.06.018
更新日期:2014-08-01 00:00:00
abstract::Recent studies have suggested a clinical significance to the detection of anti-major histocompatibility complex class I-related chain A (MICA) antibodies in transplantation. We have developed an eplet-based version of the HLAMatchmaker algorithm to assess the epitope specificity of these antibodies. Molecular viewing ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2008.10.003
更新日期:2008-12-01 00:00:00
abstract::Macrophages can be polarized into classically (CAM) or alternatively (AAM) activated macrophages with IFN-γ or IL-4, respectively. CAM are associated with type 1 immune responses and are implicated in autoimmunity; AAM are associated with type 2 responses and are implicated in allergic diseases. An impediment in inves...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2013.08.267
更新日期:2013-12-01 00:00:00
abstract::HLA-B703 (BPOT) is a B7-like antigen segregating within families. To determine the complete B*0703 nucleotide sequence, HLA-ABC-cDNAs derived from the EBV cell line POT71 have been cloned. Four B*0703-positive clones were sequenced to obtain an accurate consensus sequence. With respect to the isoelectric focusing-iden...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(93)90533-7
更新日期:1993-10-01 00:00:00