Abstract：
:A comprehensive system for genetic typing of the HLA class I A locus is described, based on PCR amplification and typing with nonradioactively labeled SSO probes. Exons 1-3 of the A locus are amplified and typing is performed with a set of 30 nonradioactively labeled oligonucleotide probes. This system resolves 34 of 39 known alleles and 561 (94%) of 595 possible genotypes. Among a sample of 354 individuals from Sweden and China, 97.5% of the genotypes were resolved. Probes were directed preferentially at replacement substitutions in foreign antigen-binding sites, in order to detect not only the known alleles but also new combinations of polymorphic motifs, indicative of previously unrecognized alleles. Three individuals were found with a new combination of polymorphic motifs, suggesting the presence of at least one previously undescribed allele in the populations sampled. This typing system is useful for disease association studies, tissue typing, and in forensic medicine.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
Allen M,Liu L,Gyllensten Udoi
10.1016/0198-8859(94)90018-3subject
Has Abstractpub_date
1994-05-01 00:00:00pages
25-32issue
1eissn
0198-8859issn
1879-1166pii
0198-8859(94)90018-3journal_volume
40pub_type
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