Abstract：
:It has been reported that collagen II (CII) derived peptide CII263-272 induced T-cell activation via its amino acids responsible for T-cell receptor (TCR) recognition. The impact of substitution of the TCR contacting amino acids of CII263-272 on T-cell activation was evaluated in this study using a panel of altered CII263-272 peptides. Computer modeling revealed that the side chains of 263F and 266E in CII263-272 were coupled with amino acids on alpha1 and beta1 chains of HLA-DR1 or -DR4, mainly via hydrogen bonds, whereas the side chains of 267Q and 270K protrude out of the cleft and might be recognized by TCR. Intracellular delivery of the altered peptides, and their binding to HLA-DR1 and -DR4 molecules on cell surface, were demonstrated by confocal microscopy and flow cytometry. The results also revealed that the substitution of 267Q, 268G, 269P, and 270K individually or consecutively by alanine (A) or glycine (G) led to weak or non-T-cell responses. Furthermore, the altered peptides with 270K substitution (270A) or with consecutive substitution of 268G, 269P, and 270K (sub268-270) dramatically inhibited T-cell activation. It is suggested that the altered peptides derived from CII263-272 with substitution of amino acids responsible for TCR contact might be of inhibitory effect on T-cell responses.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
Zhou Q,Cheng Y,Lü H,Zhou W,Li Zdoi
10.1016/s0198-8859(03)00143-5subject
Has Abstractpub_date
2003-09-01 00:00:00pages
857-65issue
9eissn
0198-8859issn
1879-1166pii
S0198885903001435journal_volume
64pub_type
杂志文章相关文献
HUMAN IMMUNOLOGY文献大全abstract：:In this study we have examined the results of probing with synthetic oligomers at the DR beta III locus, together with restriction fragment length polymorphisms defined by BglII digestion and a cDNA DR alpha probe, and Taq 1 digestion and a genomic DQ alpha probe. We have demonstrated heterogeneity of the human leukoc...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(87)90018-8
更新日期：1987-12-01 00:00:00
abstract：:The role of HLA antigens in lymphocyte differentiation is strongly suggested by the existence of a recently identified immunodeficiency associated with, and probably resulting from, the lack of expression of HLA-A, B, and C antigens as well as beta2 microglobulin on various cells of hematopoietic origin. This "bare ly...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(81)90061-6
更新日期：1981-03-01 00:00:00
abstract：:In the present study, we explored the specific requirements for lysis of human activated T cells by CD4+ CTLs. This was achieved by using human CD4+ T cell lines or clones specific for a peptidic fragment of influenza virus as both CTL effectors and target T cells (TTCs). Our results further establish that human activ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(92)90094-4
更新日期：1992-09-01 00:00:00
abstract：:The cytotoxic T-lymphocyte antigen 4 (CTLA4, CD152) gene is a positional and functional candidate gene to susceptibility to systemic lupus erythematosus (SLE) because CTLA4 gene maps in the described SLE risk region 2q33 and CTLA4 molecule has an inhibitory effect on T-cell activation. Several polymorphisms have been ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(03)00171-x
更新日期：2003-10-01 00:00:00
abstract：:TCRBV CDR3 repertoire diversity was analyzed in a cross-sectional study of HIV-infected individuals by CDR3 fingerprinting/spectratyping and single strand conformation polymorphism (SSCP). Most TCRBV families were detected in CD4+ cells of HIV-infected patients with CD4 counts ranging from 35 to 1103. In patients with...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(01)00361-5
更新日期：2002-01-01 00:00:00
abstract：:The Human Leukocyte Antigen-G has immunomodulatory function and its expression has been associated with several diseases. In our study we analyzed HLA-G polymorphisms in order to evaluate their possible association with susceptibility to celiac disease development. A total of 420 celiac patients and 509 controls were ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2014.12.006
更新日期：2015-01-01 00:00:00
abstract：:Behçet's disease (BD) has been established to be associated with HLA-B51. However, it has not been revealed whether the HLA-B51 gene itself or another gene located near the HLA-B gene is directly involved in the pathogenesis of BD. Previously, using Japanese BD patients, our group has narrowed down a BD-causative gene...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(00)00246-9
更新日期：2001-02-01 00:00:00
abstract：:In this paper we briefly review three common immunological misconceptions that feature prominently among anti-vaccinationists, and in turn, fuel patient and parental concerns, questions, and fears about vaccines. In particular, this Perspective covers a brief history of the anti-vaccine movement, and three common fals...
journal_title：Human immunology
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.humimm.2012.03.014
更新日期：2012-08-01 00:00:00
abstract：:Two hundred and three sera obtained from multiparous women were screened to determine the frequency of the appearance of antibodies reactive with PHA-activated T lymphocytes, but not with resting B or T cells from the same donor. The panel of cells on which the sera were tested consisted of lymphocytes from 38 unrelat...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(80)90110-x
更新日期：1980-12-01 00:00:00
abstract：:Recent studies have suggested a clinical significance to the detection of anti-major histocompatibility complex class I-related chain A (MICA) antibodies in transplantation. We have developed an eplet-based version of the HLAMatchmaker algorithm to assess the epitope specificity of these antibodies. Molecular viewing ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2008.10.003
更新日期：2008-12-01 00:00:00
abstract：:Rat soluble MHC class I synthesis was studied at both RNA and protein levels to determine whether multiple forms of soluble MHC class I molecules are produced by different mechanisms. RT-PCR and sequencing of MHC class I transcripts identified an alternatively spliced nonclassical MHC class I gene product, lacking bot...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(98)00039-1
更新日期：1998-07-01 00:00:00
abstract：:We describe a two-step clonal culture assay system for human lymphohematopoietic progenitors present in umbilical cord blood which are capable of differentiation along both myeloid and B-lymphoid lineages. Human cord blood CD34+ cells were plated in methylcellulose in the presence of stem cell factor (SCF), granulocyt...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(98)00094-9
更新日期：1999-01-01 00:00:00
abstract：:NKG2D is an important activating receptor on NK cells and T-cells and has a diverse panel of ligands (NKG2DL) which include the ULBP and RAET1 proteins. Several NKG2DL exhibit a considerable degree of genetic polymorphism, and although the functional significance of such allelic variation remains unclear, genetic vari...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2010.02.018
更新日期：2010-06-01 00:00:00
abstract：:HLA-G was described as a molecule inhibiting NK and T cells functions through its receptor, ILT2. However, most functional studies of HLA-G were so far performed on heterogeneous immune populations and regardless of ILT2 expression. This may lead to an underestimation of the effect of HLA-G. Thus, considering the immu...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2020.02.001
更新日期：2020-04-01 00:00:00
abstract：:Previously we have shown that the supratype HLA B17 BfS C4A6 B1 DR7 (17 6 1 7) carrying a C4/Bgl II RFLP correlating with C4A6 coexists in whites and Thai/Chinese. Using conventional and PFGE/Southern blotting with class II, class III, and TNF probes as well as serologic DQ subtyping, we have extended these comparison...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(88)90015-8
更新日期：1988-09-01 00:00:00
abstract：:Mannose-binding lectin (MBL) is a major, soluble, pattern-recognition molecule and an important component of the innate host defense. The role of MBL in inflammatory bowel diseases (IBDs) is controversial. We determined the prevalence of MBL deficiency in a Hungarian IBD patients' cohort, and whether it is associated ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2010.01.012
更新日期：2010-04-01 00:00:00
abstract：:Our purpose was to investigate a possible relationship between severe early childhood caries (S-ECC) and polymorphism of the mannose binding lectin gene and investigate the role of allele variant as a possible factor in the susceptibility to S-ECC. Sixty-two Chinese children with S-ECC and 68 caries-free control child...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2012.08.012
更新日期：2013-01-01 00:00:00
abstract：:Toll like receptors (TLRs) are a class of molecular pattern recognition receptors, elicits a strong inflammatory immune response against pathogens. Helicobacter pylori (H. pylori), a gram negative flagellate colonizes the human stomach, is responsible for the development of chronic gastritis and gastric carcinoma. The...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2016.12.002
更新日期：2017-02-01 00:00:00
abstract：:Natural killer cells (NK) are pivotal cells of innate immunity. They are potent antileukemic cytotoxic effectors. A defect in their cytotoxicity has been described in some hematopoietic malignancies such as acute myeloid leukemia, multiple myeloma and myelodysplastic syndromes. This defect is at least partially linked...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2015.09.010
更新日期：2015-09-01 00:00:00
abstract：:CD3+CD20+ T cells are a population of CD3+ T cells co-expressing CD20 that make up to ∼3-5% of the CD3+ T-cell compartment in the peripheral blood of human beings. In healthy individuals, CD3+CD20+ T cells are heterogeneous for containing a lower proportion of CD4+ cells, but produce higher levels of IL-17A and/or IFN...
journal_title：Human immunology
pub_type： 杂志文章,评审
doi：10.1016/j.humimm.2019.01.001
更新日期：2019-03-01 00:00:00
abstract：:This study describes the diversity of DRw6 and DR5 haplotypes in the American black population using serology, cellular typing, and restriction fragment length polymorphism (RFLP) analysis. DRw6 (DRw13 and DRw14) and DR5 (DRw11 and DRw12) haplotypes are observed at a high frequency in this population (DRw6: 32%, DR5: ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(90)90115-6
更新日期：1990-11-01 00:00:00
abstract：:The cellular basis of graft rejection and the development of strategies for specific suppression of T cell responses against allogeneic and xenogeneic transplants represents an area of active investigation. Recently, a population of MHC-class I restricted CD8+CD28- T suppressor cells (Ts) which are able to inhibit spe...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(98)00134-7
更新日期：1999-04-01 00:00:00
abstract：:Identification of mycobacterial antigens that are recognized by CD4+ Th1 cells in HLA-nonrestricted manner or in association with multiple allelic products is required to develop universally effective vaccines against mycobacterial diseases. Our studies in this direction have shown that several recombinant mycobacteri...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(99)00137-8
更新日期：2000-02-01 00:00:00
abstract：:This study aimed at evaluating the functional activation and activating receptors expression on resting, short- and long-term NK and NK-like T cells from blood of ovarian neoplasia patients. Blood from patients with adnexal benign alterations (n = 10) and ovarian cancer (grade I-IV n = 14) were collected after signed ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2014.05.010
更新日期：2014-08-01 00:00:00
abstract：BACKGROUND:Tumour necrosis factor-alpha (TNF-alpha) has been implicated in the pathogenesis of generalized aggressive periodontitis (AgP) and chronic periodontitis (CP). The objective of the present study was to evaluate the association of four TNF-alpha gene polymorphisms (-1031T/C, -857C/T, -308G/A and -238G/A) with ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2013.08.009
更新日期：2013-12-01 00:00:00
abstract：:A proinflammatory cytokine interleukin-6 (IL-6) plays an important role in the development, pathogenesis and outcome of SIRS, sepsis and septic shock. We have evaluated the role of the IL-6 gene polymorphisms in pediatric patients. A total of 421 consecutive pediatric patients admitted to the pediatric intensive care ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2007.06.003
更新日期：2007-09-01 00:00:00
abstract：:It has been known for 40 years that cytotoxic human leukocyte antigen (HLA) antibodies are associated with graft rejection. However, the complement-dependent cytotoxicity assay (CDC) used to define these clinically deleterious antibodies suffers from a lack of sensitivity and specificity. Recently, methods exploiting ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2011.07.001
更新日期：2011-10-01 00:00:00
abstract：:Rearrangements of the T-cell Rearranging Gene (TRG) or T-cell-receptor gamma-chain genes were analyzed in 24 in vivo-sensitized alloreactive T-cell clones. This analysis represents the first complete assignment of TRG gene rearrangements to given variable and joining gene segments in nonleukemic T cells and provides s...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(89)90050-5
更新日期：1989-02-01 00:00:00
abstract：:Signal transducer and activator of transcription 4 (STAT4) has been recently identified as a susceptibility gene for multiple autoimmune diseases. Here we performed a comprehensive analysis of the association between STAT4 and several different autoimmune disorders to identify potential common inflammatory principles ...
journal_title：Human immunology
pub_type： 杂志文章,meta分析
doi：10.1016/j.humimm.2013.04.034
更新日期：2013-08-01 00:00:00
abstract：:Based on CD25 expression, T follicular helper cells (Tfh) could be divided into T follicular regulatory (Tfr)-like subset (CD25+CD4+CXCR5+) and CD25- Tfh subset (CD25-CD4+CXCR5+). Patients with diffuse large B cell lymphoma (DLBCL) display high level of Tfr-like cells in blood and tumor. This Tfr-like subset could sup...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2020.05.008
更新日期：2020-08-01 00:00:00
