Abstract：
:There is considerable current interest in the application of Southern blotting and hybridization, with locus-specific HLA class II probes, to HLA typing. The work of several laboratories has shown that, under suitable conditions, restriction fragment patterns that correlate with DR and DQ serology and with DP cellular reactivities can be obtained from homozygous cell lines. Although the identification of class II specificities from restriction fragment patterns is relatively straightforward in homozygotes, the increased complexity of the patterns obtained from heterozygotes makes the interpretation considerably more difficult; this difficulty is compounded by even slight variation between and within gels. We have developed an interactive computer program that allows HLA-DR and DQ typing by the visual matching of restriction fragment pattern data with standard data derived from a panel of well-characterized homozygous cell lines. The program also uses a simple algorithm (after Southern) to estimate the fragment sizes in the unknown track. The screen display can be printed out to give a convenient record of the match; a numerical measure is also given of the goodness of fit of the new data.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
Bangham CR,Bushell A,Wood KJ,Morris PJdoi
10.1016/0198-8859(88)90087-0subject
Has Abstractpub_date
1988-02-01 00:00:00pages
125-32issue
2eissn
0198-8859issn
1879-1166pii
0198-8859(88)90087-0journal_volume
21pub_type
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