Abstract:
:Macrophages can be polarized into classically (CAM) or alternatively (AAM) activated macrophages with IFN-γ or IL-4, respectively. CAM are associated with type 1 immune responses and are implicated in autoimmunity; AAM are associated with type 2 responses and are implicated in allergic diseases. An impediment in investigating macrophage biology using primary human monocyte derived macrophages is the wide inter-donor heterogeneity and the limited quantity of cells that survive in vitro polarization. To overcome this impediment, we established a protocol to generate CAM and AAM cultures derived from the THP-1 human promonocytic cell line. In this report, we demonstrate that THP-CAM and -AAM express gene and protein markers that define their primary human monocyte derived counterparts, such as IL-1β, CXCL10, and CXCL11 for CAM, and MRC1, IL-4 and CCL22 for AAM. In addition, we demonstrate that STAT6 is selectively activated in THP-AAM which, upon LPS stimulation, have an attenuated or delayed expression of IFN-β, IFN-λ1, and IFN α/β pathway genes compared to their CAM counterparts. Taken together, these findings may help further investigate human diseases associated with the alternatively activated macrophage phenotype using this reproducible in vitro macrophage model.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
El Fiky A,Perreault R,McGinnis GJ,Rabin RLdoi
10.1016/j.humimm.2013.08.267subject
Has Abstractpub_date
2013-12-01 00:00:00pages
1524-30issue
12eissn
0198-8859issn
1879-1166pii
S0198-8859(13)00492-8journal_volume
74pub_type
杂志文章相关文献
HUMAN IMMUNOLOGY文献大全abstract::A large percentage of healthy individuals (50-90%) is chronically infected with Cytomegalovirus (CMV). Over the past few years, several techniques were developed in order to monitor CMV-specific T-cell responses. In addition to the identification of antigen-specific T cells with peptide-loaded MHC complexes, most of t...
journal_title:Human immunology
pub_type: 杂志文章,评审
doi:10.1016/j.humimm.2004.02.012
更新日期:2004-05-01 00:00:00
abstract::HLA genotyping by polymerase chain reaction (PCR) has some inherent labor-intensive and effort-demanding limitations. To overcome them, we have developed a real-time PCR with hybridization probes approach able to obtain a medium-low resolution HLA-B genotyping with fewer tubes and probes and with a shorter time requir...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2006.02.038
更新日期:2006-04-01 00:00:00
abstract::In this study, we evaluated the leukocyte density and composition in the human cycling endometrium with uterine fibroids (UF). The endometrium with neighboring nodule (NN group, n = 62), autologous endometrium without NN (non-NN group, n = 62), and allogeneic endometrium without UF (non-UF group, n = 24) were immunost...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2009.11.014
更新日期:2010-02-01 00:00:00
abstract::Dendritic cells (DCs) play an important role in inducing immune tolerance. Inhibitory receptors, such as ILT2/CD85j, are involved in the tolerogenic effect of DCs and previous studies have indicated the important role of these receptors on the pathogenesis of autoimmune diseases. The aim of this study was to evaluate ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2013.05.006
更新日期:2013-09-01 00:00:00
abstract::Positive selection of immature thymocytes is a developmental process in which TCR ligation with low avidity induces generation of mature T cells. In mouse thymocytes, CD4(+)8(+) double-positive (DP) cells which were treated with a proper combination of calcium ionophore ionomycin and phorbol 12-myristate 13-acetate (P...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(01)00276-2
更新日期:2001-08-01 00:00:00
abstract::From a human cDNA library constructed from consanguineous HLA-homozygous cell line AKIBA (HLA-A24, Bw52, DR2, Dw12, Cp63 Cp63: a new DP type), cDNA clones encoding the heavy and light chains of an HLA-DP Cp63 alloantigen were isolated and analyzed by restriction enzyme mapping and nucleotide sequence determination. Al...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(86)90286-7
更新日期:1986-11-01 00:00:00
abstract::The HER-2/neu protein is overexpressed in approximately 20% of human adenocarcinomas and is a defined tumor antigen in breast cancer. The purpose of this study was to evaluate the endogenous HER-2/neu specific antibody response in 57 patients with colorectal cancer. HER-2/neu specific antibodies, titer > or = 1:100, w...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(99)00003-8
更新日期:1999-06-01 00:00:00
abstract::Childhood B-cell precursor acute lymphoblastic leukemia (BCP ALL) is usually initiated in utero and is thought to progress to overt leukemia under the influence of delayed exposure to a common infection. Based on the hypothesis that polymorphic HLA-DP variants can restrict T-cell responses to infection, we previously ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2011.05.011
更新日期:2011-10-01 00:00:00
abstract::The MHC in humans is a much studied region of the genome, the genes of which display a high rate of polymorphism and a high rate of linkage disequilibrium. Four families in which intra-class-I recombination has occurred have been analyzed with six polymorphic markers between HLA-A and -B in order to determine the full...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(93)90530-e
更新日期:1993-10-01 00:00:00
abstract::The 5' cis-regulatory region of the CCR5 gene exhibits a strong signature of balancing selection in several human populations. Here we analyze the polymorphism of this region in Amerindians from Amazonia, who have a complex demographic history, including recent bottlenecks that are known to reduce genetic variability....
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2010.05.022
更新日期:2010-09-01 00:00:00
abstract::The aim of this study was to analyze the relationship between the IFNG +874 T/A and IL28B (rs12979860) C/T polymorphisms and the secretion of IFNG by CD8+ T cells after stimulation with cytomegalovirus (CMV) peptides, measured using QuantiFERON-CMV (QF-CMV) assay. A total of 184 CMV-seropositive solid organ transplant...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2018.03.009
更新日期:2018-06-01 00:00:00
abstract::In an attempt to develop a suitable model for increasing the yield of human anti-HLA mAbs, we have used mice with SCID for i.p. injection of two human-mouse heterohybridomas. HMP1 hybridoma secretes a DQB1*0201 allele-specific human mAb whereas HMP12 secretes a human mAb recognizing the DRB1*1101, 1102, 1103, and 1104...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(92)90008-b
更新日期:1992-12-01 00:00:00
abstract::Septic patients develop immune dysfunctions, the intensities and durations of which are associated with deleterious outcomes. LILRB2 (leukocyte immunoglobulin-like receptors subfamily B, member 2), an inhibitory member of the LILR family of receptors, is known for its immunoregulatory properties. In a microarray study...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2017.03.010
更新日期:2017-01-01 00:00:00
abstract::We compared the T cell receptor (TCR) V beta gene family repertoire in peripheral blood mononuclear cells (PBMC) and lymph node (LN) cells from 7 human immunodeficiency virus (HIV)-infected patients and 3 seronegative healthy controls. Virtually all the V beta family specificities were represented in patient PBMC and ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(97)00205-x
更新日期:1997-10-01 00:00:00
abstract::IDDM is known to be associated with genes of HLA complex, particularly alleles of HLA-DQ. The 40-kb TAP gene complex is located approximately 150 kb centrometric to the DQB1 locus. The TAP1-TAP2 protein heterodimer is required for normal expression levels of class I, molecules on the surface of cells. While present ev...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(94)00124-9
更新日期:1995-05-01 00:00:00
abstract::A novel cell surface molecule, DN4, defined by an mAb raised against human thymic epithelial cells, showed a specificity for epithelial cells of the thymic cortex. This antigen was not expressed at detectable levels on any other types of tissues in the human body except for the thymus and bone marrow. Immunohistochemi...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(95)00168-9
更新日期:1996-02-01 00:00:00
abstract::Genes encoding for prolactin (PRL) and its receptor (PRLR) are possible candidates for multiple sclerosis (MS) and systemic lupus erythematosus (SLE) susceptibility. In fact: (1) a prolactin secretion dysfunction has been described in several autoimmune diseases including SLE and MS and their animal models; (2) both P...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(02)00804-2
更新日期:2003-02-01 00:00:00
abstract::Pronase treatment is used in the flow cytometry crossmatch (FCXM) to prevent nonspecific antibody binding on B cells. However, we have observed unexpected positive results with pronase-treated T cells in human immunodeficiency virus (HIV)-infected patients. In this study, 25 HIV-infected patients without HLA antibodie...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2016.04.014
更新日期:2016-06-01 00:00:00
abstract::Using the soft-agar colony assay, we have generated three MT3-associated clones: HJ1, HJ13, and HJ39, from an MLR combination of two unrelated individuals. Another clone, HJ37, appeared to recognize a novel HLA-D determinant. PLT inhibition studies with monoclonal anti-Ia-like antibodies (Mab) were conducted on clones...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(83)90066-6
更新日期:1983-02-01 00:00:00
abstract::The aim of this work was to investigate the longitudinal evolution of plasmatic soluble HLA-G (sHLA-G: shed HLA-G1 plus HLA-G5) during pregnancy, and if peripheral maternal antigen presenting cells (APC) can be a source of sHLA-G. Blood samples were obtained from 45 volunteers during normal pregnancy, 8 of them monthl...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2007.04.007
更新日期:2007-08-01 00:00:00
abstract::CD8-positive γδ T lymphocytes (GDCD8(+)) are specifically increased in peripheral blood of Behçet's disease (BD) patients. GDCD8(+) have shown a T regulatory (Treg) function in autoimmune experimental models, human tumor infiltrates and intestinal intraepithelial lymphocytes from celiac patients. The aim of this study...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2015.10.001
更新日期:2016-01-01 00:00:00
abstract::The role of HLA-G in several clinical conditions related to reproduction has been investigated. Important polymorphisms have been found within the 5'URR and 3'UTR regions of the HLA-G promoter. The aim of the present study was to investigate 16 SNPs in the 5'URR and 14-bp insertion/deletion (ins/del) polymorphism loca...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2012.06.002
更新日期:2012-09-01 00:00:00
abstract::Polymorphisms of interleukin-23 receptor (IL23R) gene have recently been reported to be associated with the susceptibility to several immune-related diseases. The aim of this study was to determine the association of IL23R polymorphisms with Vogt-Koyanagi-Harada (VKH) syndrome, a disease presumably mediated by autoimm...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2010.01.026
更新日期:2010-04-01 00:00:00
abstract::Many factors induce or enhance expression of major histocompatibility complex class I and class II molecules on various cell types. Human T lymphocytes are class II negative in the resting state but show expression of class II molecules following activation. We analyzed the modulating capacity of the lymphokines recom...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(91)90101-e
更新日期:1991-08-01 00:00:00
abstract::The breakdown of tolerance to autologous bacterial flora has been implicated as a major factor contributing to the initiation and perpetuation of chronic inflammation in inflammatory bowel diseases (IBD). To test whether bacterial DNA is at the origin of inflammation in IBD, we have examined the response of lamina pro...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2003.12.013
更新日期:2004-03-01 00:00:00
abstract::The reactivity of monoclonal antibodies (mAbs) R1, S1, and S5, shown previously to recognize polymorphic epitopes on HLA-DQ molecules, have been found to correlate with the presence of certain DQB1 alleles. mAb S5 reacts with cells expressing DQB1*0503, 0601, 0602, 0603, or 0604 alleles while R1 and S1 react with all ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(91)90009-x
更新日期:1991-06-01 00:00:00
abstract::In our earlier studies, single nucleotide polymorphisms (SNPs) associated with anti-inflammatory cytokines were found to influence risk for breast cancer in western Indian women. Analysis of Interleukin 6 (IL-6) -174G>C polymorphism in this cohort (patients = 182; controls = 236) suggested a protective role for IL-6 -...
journal_title:Human immunology
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.humimm.2014.06.018
更新日期:2014-08-01 00:00:00
abstract::Haplotype V at the Y-chromosome specific DNA polymorphism (p49/TaqI) was reported in a study concerning 487 males originating from five different geographic locations in Iberia and North Africa. The highest frequency of haplotype V (68.9%) was previously observed in Berbers from Morocco, and it was previously establis...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(01)00287-7
更新日期:2001-09-01 00:00:00
abstract::Early allograft function after lung transplantation is variable. Clinical criteria have limited predictive value for early graft function. Recipient immunologic state before LTx may affect early lung function. We investigated the association between pretransplantation soluble CD30 (sCD30), a marker of Th2-type T-cell ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2008.12.005
更新日期:2009-02-01 00:00:00
abstract::As rheumatoid arthritis (RA) is an HLA-DR associated autoimmune disease and soluble HLA-DR (sHLA-DR) molecules have the capacity to regulate the immune response, we studied the sHLA-DR levels in RA patients in view of therapy modalities and clinical and biologic parameters of disease activity. For this sHLA-DR concent...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(02)00431-7
更新日期:2002-09-01 00:00:00