Flow cytometry crossmatch reactivity with pronase-treated T cells induced by non-HLA autoantibodies in human immunodeficiency virus-infected patients.

abstract：:In this study, the polymorphisms of the HLA DMA and DMB genes in patients with rheumatoid arthritis (RA) were examined.DMA and DMB typing was performed in 120 white RA patients from eastern France and 100 healthy controls, using PCR-SSO (sequence specific oligonucleotide probes) method for DMA determination and PCR-RF...

journal_title：Human immunology

authors： Toussirot E,Sauvageot C,Chabod J,Ferrand C,Tiberghien P,Wendling D

更新日期：2000-03-01 00:00:00

abstract：:Detection and avoidance of donor-reactive antibodies in the sera of potential organ transplant recipients is key to a successful transplant outcome. Techniques of antibody detection that use flow cytometry are more sensitive than those that rely upon a visual determination of cytotoxicity. However, as conventionally p...

journal_title：Human immunology

authors： Wetzsteon PJ,Head MA,Fletcher LM,Lye WC,Norman DJ

更新日期：1992-10-01 00:00:00

abstract：:In the present study, we explored the specific requirements for lysis of human activated T cells by CD4+ CTLs. This was achieved by using human CD4+ T cell lines or clones specific for a peptidic fragment of influenza virus as both CTL effectors and target T cells (TTCs). Our results further establish that human activ...

journal_title：Human immunology

authors： Eljaafari A,Dorval I,Zeliszewski D,Le Gac S,Sterkers G

更新日期：1992-09-01 00:00:00

abstract：:To identify disease-specific T cell changes that occur in Crohn's disease (CD) the T-cell receptor (TCR) BV repertoires of lamina propria lymphocytes (LPL) from both disease-active and disease-inactive colonic tissue of three CD patients were compared by a quantitative polymerase chain reaction (qPCR) and CDR3 length ...

journal_title：Human immunology

authors： Gulwani-Akolkar B,Akolkar PN,Minassian A,McKinley M,Fisher S,Silver J

更新日期：1996-06-01 00:00:00

abstract：:Behçet's disease (BD) has been established to be associated with HLA-B51. However, it has not been revealed whether the HLA-B51 gene itself or another gene located near the HLA-B gene is directly involved in the pathogenesis of BD. Previously, using Japanese BD patients, our group has narrowed down a BD-causative gene...

journal_title：Human immunology

authors： Mizuki N,Yabuki K,Ota M,Verity D,Katsuyama Y,Ando H,Onari K,Goto K,Imagawa Y,Mandanat W,Fayyad F,Stanford M,Ohno S,Inoko H

更新日期：2001-02-01 00:00:00

abstract：:The pattern of lymphocyte growth from endomyocardial biopsies in 55 heart transplant recipients was shown to be correlated with the subsequent development of graft coronary disease. Persistent lymphocyte growth was observed in 39 patients, and 15 of these growers (or 41%) developed graft coronary disease. In contrast,...

journal_title：Human immunology

authors： Kaufman CL,Zeevi A,Kormos RL,Zerbe TR,Keenan RJ,Uretsky BF,Griffith BP,Hardesty RL,Duquesnoy RJ

更新日期：1990-06-01 00:00:00

abstract：:Alloantibodies are known to influence transplant outcomes. Apart from human leukocyte antigens (HLA), non-HLA targets have been suggested to play a significant role, but little is known about their nature. Here, we present a novel method for identification and characterization of cell surface antigens bound by allorea...

journal_title：Human immunology

authors： Heinold A,Kuehl B,Brenner-Weiss G,Opelz G,Tran TH

更新日期：2010-05-01 00:00:00

abstract：:Attempts to predict outcome of bone marrow transplantation based on killer immunoglobulin-like receptor (KIR) and HLA genotyping have yielded discordant results. To better understand the factors involved, we investigated natural killer (NK) cell function and correlated it with genetics and expression of inhibitory KIR...

journal_title：Human immunology

authors： Han M,Fallena M,Guo Y,Stastny P

更新日期：2007-06-01 00:00:00

abstract：:Cytomegalovirus (CMV) is a well-known cause of disease occurring after bone marrow transplantation (BMT). The manifestations of CMV range from asymptomatic infection, defined as active CMV replication in the blood in the absence of clinical manifestations or organ failure abnormalities, to CMV disease, characterized b...

journal_title：Human immunology

authors： Castagnola E,Cappelli B,Erba D,Rabagliati A,Lanino E,Dini G

更新日期：2004-05-01 00:00:00

abstract：PURPOSE:When donor specific HLA antibodies (DSA) are identified, the predictive value of whether a certain strength of reactivity (mean fluorescence intensity, MFI) leads to a positive crossmatch is uncertain. To determine this, we compared the DSA MFI results we generated locally for nationally distributed proficiency...

journal_title：Human immunology

authors： Wrenn SM,Marroquin CE,Hain DS,Harm SK,Pineda JA,Hammond PB,Shah DH,Hillyard SE,Fung MK

更新日期：2018-08-01 00:00:00

abstract：:Peptide binding to HLA-DR molecules in intracellular compartments is facilitated by HLA-DM molecules, present in most types of antigen-presenting cells. Allorecognition of DR specificities represents a form of T cell recognition of the MHC-peptide complex which in some cases is influenced by peptide binding. DRA and D...

journal_title：Human immunology

authors： Drover S,Kovats S,Masewicz S,Blum JS,Nepom GT

更新日期：1998-02-01 00:00:00

abstract：:The metalloproteinase (MPase)-mediated pathway of MHC class I processing is a distinct cellular mechanism that generates soluble HLA proteins. It has been implicated in modulation of immune responses induced during transplantation events. It is, therefore, important to define the characteristics of soluble HLA species...

journal_title：Human immunology

authors： Demaria S,Bushkin Y

更新日期：2000-12-01 00:00:00

abstract：:A flow cytometric method of simultaneously screening both HLA Class I and Class II panel reactive antibodies (PRA) was developed using a pool of 30 different Class I and 30 different Class II microbeads coated with purified HLA antigens. The antibodies in the serum that react specifically to the coated HLA antigens ar...

journal_title：Human immunology

authors： Pei R,Wang G,Tarsitani C,Rojo S,Chen T,Takemura S,Liu A,Lee J

更新日期：1998-05-01 00:00:00

abstract：:The HLA-DR4 subtypes Dw14 and Dw4 are T-cell-defined allospecificities encoded by the DRB1*0404 and DRB1*0401 genes, respectively. Although these allelic subtypes differ in only two amino acids, allorecognition between Dw14 and Dw4-positive individuals is brisk. This provides an opportunity to analyze T-cell receptor ...

journal_title：Human immunology

authors： Yamanaka K,Kwok WW,Mickelson EM,Masewicz S,Nepom GT

更新日期：1992-01-01 00:00:00

abstract：:DNA sequence-based typing at the HLA-A, -B, -C, -DPA1, -DPB1, -DQA1, -DQB1, -DRB1, and -DRB3/4/5 loci was performed on samples provided by 159 individuals from the Worcester region of the Western Cape province of South Africa. The purpose of the study was to characterize allele frequencies in the local population, to ...

journal_title：Human immunology

authors： Grifoni A,Sidney J,Carpenter C,Phillips E,Mallal S,Scriba TJ,Sette A,Lindestam Arlehamn CS

更新日期：2018-03-01 00:00:00

abstract：:Genetic variations in chemokine genes influence the chemoattractive properties of T cells which may be associated with outcome of infections. In present study, we have investigated the regulatory role played by In1.1T/C (rs2280789) polymorphism of CCL5 and -135G/A (rs56061981) polymorphism of CXCL10 gene on intracellu...

journal_title：Human immunology

authors： Singh B,Anbalagan S,Selvaraj P

更新日期：2017-01-01 00:00:00

abstract：:The presentation of donor-derived MHC peptides by recipient APCs to T cells is an essential component of the rejection of allografts (indirect allorecognition). Initial alloreactive T cell response is confined to a few well processed and presented dominant determinants on donor MHC. However, during long-term graft rej...

journal_title：Human immunology

authors： Benichou G,Malloy KM,Tam RC,Heeger PS,Fedoseyeva EV

更新日期：1998-09-01 00:00:00

abstract：:Based on CD25 expression, T follicular helper cells (Tfh) could be divided into T follicular regulatory (Tfr)-like subset (CD25+CD4+CXCR5+) and CD25- Tfh subset (CD25-CD4+CXCR5+). Patients with diffuse large B cell lymphoma (DLBCL) display high level of Tfr-like cells in blood and tumor. This Tfr-like subset could sup...

journal_title：Human immunology

authors： Ma X,Zha J,He J,Chen L,Huang J,Wu W,Tian P,Qian BH,Yu L,Jiang Y,Xu B

更新日期：2020-08-01 00:00:00

abstract：:Induced Treg with the phenotype CD4(+)CD25(+)Foxp3(+)IFNγ(+) were shown to be associated with good long-term graft outcome in renal transplant recipients and inhibition of allogeneic T-cell responses in vitro. In the present study, we investigated whether apoptosis and Fas/FasL-dependent pathways contribute to the inh...

journal_title：Human immunology

authors： Daniel V,Sadeghi M,Wang H,Opelz G

更新日期：2013-02-01 00:00:00

abstract：:Galectins constitute an evolutionary conserved family that binds to β-galactosides. There is growing evidence that galectins are implicated in essential biological processes such as cellular communication, inflammation, differentiation and apoptosis. Galectin-3 is one of the best-known galectins, which is found in ver...

journal_title：Human immunology

authors： Zeinali M,Adelinik A,Papian S,Khorramdelazad H,Abedinzadeh M

更新日期：2015-10-01 00:00:00

abstract：:We compared the immunohistochemical reaction patterns of HLA-G-specific antibodies 87G, 4H84, G233, 16G1, and BFL.1 on human placentas under three different preparative conditions and on cryosections of other human tissues. Human and murine cell lines, either naturally expressing or transfected with HLA-G, were analyz...

journal_title：Human immunology

authors： Blaschitz A,Hutter H,Leitner V,Pilz S,Wintersteiger R,Dohr G,Sedlmayr P

更新日期：2000-11-01 00:00:00

abstract：:To calculate reliable HLA gene and haplotype frequencies of bone marrow donors in various regions in the world, we have analyzed the HLA-A, -B, and -DR phenotype frequencies of 18 bone marrow donor registries with a total of more than 300,000 HLA-A, -B-typed donors. These registries were included in the 22nd edition o...

journal_title：Human immunology

authors： Schipper RF,D'Amaro J,Bakker JT,Bakker J,van Rood JJ,Oudshoorn M

更新日期：1997-01-01 00:00:00

abstract：:RING 11, a second transport-associated gene (TAP2), has been recently identified in the DR-DP interval of the human class II region. Two predominant alleles, TAP2A and TAP2B, differing by 17 amino acids at the C-terminus of the ATP-binding domain are present in the Caucasoid population at frequencies of 79% and 21%, r...

journal_title：Human immunology

authors： Eiermann TH,Fakler J,Goldmann SF,Böhm BO

更新日期：1993-11-01 00:00:00

abstract：:Gene copy number variation (CNV) of the CC Chemokine ligand 3-Like-1 (CCL3L1) gene located on chromosome 17q12 has been associated with many diseases, including viral infections and autoimmune diseases. High sequence homology between CCL3L1 and three other related genes within the same cluster, CCL3, CCL3L2, and CCL3L...

journal_title：Human immunology

authors： Perry RT,Nyaku MK,Aissani B,Shrestha S

更新日期：2014-08-01 00:00:00

abstract：:Preformed and de novo donor-specific HLA antibodies (DSA) have been associated with allograft dysfunction and failure. The application of solid-phase methods have increased the sensitivity and specificity of antibody detection; however the clinical significance of these DSA is under evaluation. In the present study, w...

journal_title：Human immunology

authors： Zeevi A,Lunz JG 3rd,Shapiro R,Randhawa P,Mazariegos G,Webber S,Girnita A

更新日期：2009-08-01 00:00:00

abstract：:It is suggested that interleukin 10 (IL-10), as a modulator of immune response, is likely to influence the elimination of hepatitis C virus (HCV), the progression of chronic hepatitis C (CHC) and the response to interferon-based therapy in CHC patients. The aim of the study was to analyze the association of single nuc...

journal_title：Human immunology

authors： Świątek-Kościelna B,Kałużna E,Strauss E,Januszkiewicz-Lewandowska D,Bereszyńska I,Wysocki J,Rembowska J,Barcińska D,Antosik D,Mozer-Lisewska I,Nowak J

更新日期：2017-02-01 00:00:00

abstract：:The HLA-DRB3 gene, which encodes the supertypic HLA-DRw52 antigen, has been shown to have limited polymorphism. The alleles at this locus are also in linkage disequilibrium with the alleles at the DRB1 locus. We have studied 16 DRw11 and three DRw12 haplotypes in the South African populations. Five of the DRw11,DQw7 h...

journal_title：Human immunology

authors： Martell RW,Oudshoorn M,Arendse B,du Toit ED

更新日期：1990-05-01 00:00:00

abstract：:This present review concentrates on the recent results investigating the role of certain cytokine gene polymorphisms, including tumor necrosis factor alpha, interferon gamma, interleukin-6 (IL-6), IL-10, and IL-1 receptor antagonist, in allogeneic stem cell transplantation. The review discusses their potential role in...

journal_title：Human immunology

authors： Dickinson AM,Cavet J,Cullup H,Wang XN,Sviland L,Middleton PG

更新日期：2001-11-01 00:00:00

abstract：:Despite the reports of dysfunction of the lytic abilities of CD8(+) T cells during human immunodeficiency virus-1 (HIV-1) disease progression, the effects of infection on the noncytolytic functions of CD8(+) T cells have not been well characterized to date. We examined the effect of HIV-1 infection on the cytokine and...

journal_title：Human immunology

authors： Gulzar N,Diker B,Balasubramanian S,Jiang JQ,Copeland KF

更新日期：2011-11-01 00:00:00

abstract：:CTLA-4 inhibitory molecule plays an important role in regulating T cell activation. It is considered a crucial element in keeping the immune balance and has been implicated in cancer, autoimmunity and transplantation immunology. Inconsistent observations are reported regarding its association with hematopoietic stem c...

journal_title：Human immunology

authors： Mossallam GI,Samra MA

更新日期：2013-12-01 00:00:00