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Simultaneous HLA Class I and Class II antibodies screening with flow cytometry.

Abstract：

:A flow cytometric method of simultaneously screening both HLA Class I and Class II panel reactive antibodies (PRA) was developed using a pool of 30 different Class I and 30 different Class II microbeads coated with purified HLA antigens. The antibodies in the serum that react specifically to the coated HLA antigens are detected by using a FITC-conjugated antibody against human IgG. Percent PRA can be determined by the percentage of microbeads that react positively to the serum. There is no cross-reactivity between the Class I and Class II microbeads. A mixture of Class I and Class II microbeads can be distinguished by their different fluorescent properties on the flow cytometry analysis. Thus, both Class I and Class II PRA can be detected from a single tube reaction.

journal_name

Hum Immunol

journal_title

Human immunology

authors

Pei R,Wang G,Tarsitani C,Rojo S,Chen T,Takemura S,Liu A,Lee J

doi

10.1016/s0198-8859(98)00020-2

subject

Has Abstract

pub_date

1998-05-01 00:00:00

pages

313-22

issue

5

eissn

0198-8859

issn

1879-1166

pii

S0198-8859(98)00020-2

journal_volume

59

pub_type

杂志文章

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