Abstract:
:Cell-mediated cytotoxicity involves a localized secretory process in which lytic agents stored in specialized granules of the effector cells are released upon contact with the appropriate target cell membrane and cause membrane damage. The protonophore carbonylcyanide m-chlorophenylhydrazone (CCCP) inhibits cytotoxicity of natural killer cells, cytotoxic T-lymphocytes and lymphokine-activated killer cells. This inhibition is due to an effect of CCCP on the cytolytic cells, rather than on their targets, and is reversible. Treatment with CCCP does not inhibit the formation of effector-target conjugates, but seems to affect the programming of the effector cells for lysis. CCCP only inhibits lysis if added during a certain period of the lytic cycle: it has an effect only if added before, or within 5 minutes of the initiation of killing by a pulse of Ca++. Effector cells treated with CCCP retain their characteristic beta-glucuronidase-positive granules, but in the presence of the drug, these are no longer oriented to face the contact area with the target cell membrane.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
McKinnon KP,Dunlevy JR,Dawson JR,Argon Ydoi
10.1016/0198-8859(88)90039-0subject
Has Abstractpub_date
1988-06-01 00:00:00pages
81-95issue
2eissn
0198-8859issn
1879-1166pii
0198-8859(88)90039-0journal_volume
22pub_type
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