Abstract:
:Preformed and de novo donor-specific HLA antibodies (DSA) have been associated with allograft dysfunction and failure. The application of solid-phase methods have increased the sensitivity and specificity of antibody detection; however the clinical significance of these DSA is under evaluation. In the present study, we summarize six cases (four renal transplant recipients, one multivisceral recipient, and one heart-and-lung transplant recipient) to illustrate the role of the histocompatibility laboratory in providing the most comprehensive workup to assess the risk of graft dysfunction associated with antibody-mediated rejection (AMR). These cases illustrate the potential risk assessment for AMR in various situations: (1) in patients exhibiting low levels of DSA pretransplantation; (2) protocol immunosuppression minimization during stepwise weaning; and (3) desensitization protocols. Furthermore, increased sensitivity of DSA determination is indicated for the interpretation of focal C4d and its clinical significance. The clinical relevance of monitoring for circulating DSA with solid-phase single-antigen assays is also discussed. These cases exemplify the rationale for all patients to be monitored for DSA post-transplantation, with the frequency adjusted based on the individual risk for AMR.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
Zeevi A,Lunz JG 3rd,Shapiro R,Randhawa P,Mazariegos G,Webber S,Girnita Adoi
10.1016/j.humimm.2009.06.009subject
Has Abstractpub_date
2009-08-01 00:00:00pages
645-50issue
8eissn
0198-8859issn
1879-1166pii
S0198-8859(09)00155-4journal_volume
70pub_type
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