Abstract：
:Detection and avoidance of donor-reactive antibodies in the sera of potential organ transplant recipients is key to a successful transplant outcome. Techniques of antibody detection that use flow cytometry are more sensitive than those that rely upon a visual determination of cytotoxicity. However, as conventionally performed, flow-cytometric crossmatches do not distinguish between cytotoxic (complement fixing) and noncytotoxic antibodies because both types of antibodies can bind to a cell and be detected by laser-activated fluorochrome photon emission. In 1989 we described two techniques for detecting cytotoxic antibodies using flow-cytometric techniques [1]. In 1990, we expanded the application of these new techniques that we called flow cytotoxicity assays or "Flow-Tox" [2]. Flow-Tox crossmatches demonstrate an increase in both sensitivity and specificity over conventional cytotoxicity crossmatches.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
Wetzsteon PJ,Head MA,Fletcher LM,Lye WC,Norman DJdoi
10.1016/0198-8859(92)90016-gsubject
Has Abstractpub_date
1992-10-01 00:00:00pages
93-9issue
2eissn
0198-8859issn
1879-1166pii
0198-8859(92)90016-Gjournal_volume
35pub_type
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