Abstract:
:The development of solid phase immunoassays using solubilized HLA molecules as targets has provided a means of detecting HLA-specific antibodies that overcomes many of the shortcomings of lymphocyte based assays. We have evaluated a commercially available assay, the GTI QuikID (QID), that uses solubilized class I molecules from 40 subjects selected for their HLA phenotype, to characterize HLA-specific antibodies. We tested 595 sera from 319 subjects and compared the results obtained with QID to those obtained with cytotoxicity (CYT) and with GTI QuikScreen (QS) as well as to historic data. The correlation of QID with CYT (r = 0.54) was comparable to that between QID and QS (r = 0.60). The majority of disparities between QS and QID were apparent false negatives with QID that could be overcome by analyzing QID data at lower cutoff values. In contrast, most of the disparities between QID and CYT were false negatives in CYT due to the relatively low sensitivity of that assay. As expected, the ELISA was more sensitive (97%) than CYT (78%) but had a somewhat lower specificity (87% vs. 92%) due, most likely, to selection of sera that excluded most sera that were known to be nonspecific by CYT. Determination of antibody specificity could be achieved quickly by manual analysis of the QID data because of the way the data are presented by the manufacturer's software. Interestingly, the frequencies of different antibodies detected by ELISA differed from those detected by CYT with ELISA identifying more sera containing antibodies to both A and B locus antigens.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
Zachary AA,Delaney NL,Lucas DP,Leffell MSdoi
10.1016/s0198-8859(00)00254-8subject
Has Abstractpub_date
2001-03-01 00:00:00pages
228-35issue
3eissn
0198-8859issn
1879-1166pii
S0198-8859(00)00254-8journal_volume
62pub_type
杂志文章相关文献
HUMAN IMMUNOLOGY文献大全abstract::Human ficolin-2 (L-ficolin; FCN2) is a serum protein binding to sugar moieties of different human micro-pathogens forcing phagocytosis. Here, we investigate the clinical significance of FCN2 in African children with either mild or severe malaria (n = 130 and n = 108, respectively) from Gabon by analyzing three promote...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2010.10.003
更新日期:2011-01-01 00:00:00
abstract::Perforin (PRF1) gene mutations have been associated with Familial Hemophagocytic Lymphohistiocytosis type 2 (FHL2). Substitution p.A91V (c.272C>T) in exon 2 was first described as a neutral polymorphism. Nonetheless, recent clinical evidence and functional assays, suggest a potential pathogenic role for p.A91V, especi...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2012.10.011
更新日期:2013-01-01 00:00:00
abstract::The co-stimulatory molecule CD28 plays an important role in T-cell-mediated immune response like acute cellular liver transplant rejection. The aim of the retrospective case- control study was to examine whether the single nucleotide polymorphisms (SNPs) rs3116487, rs3116494, and rs3116496 of the CD28 gene are associa...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2020.10.002
更新日期:2020-12-01 00:00:00
abstract::Mexican Mestizos, who are the result of the admixture of Spanish, Indian, and Black genes, were analyzed for different systems. Three populations from geographical distinct areas were studied: the north (State of Nuevo Leon ), the center (State of Guanajuato), and the highlands (mainly Mexico City). Ten blood group sy...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(01)00296-8
更新日期:2001-09-01 00:00:00
abstract::It is suggested that interleukin 10 (IL-10), as a modulator of immune response, is likely to influence the elimination of hepatitis C virus (HCV), the progression of chronic hepatitis C (CHC) and the response to interferon-based therapy in CHC patients. The aim of the study was to analyze the association of single nuc...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2016.10.015
更新日期:2017-02-01 00:00:00
abstract::It has been known for some time that transplant recipients may have antibodies to endothelial cells which are not detected on lymphocytes. However, little progress has been made in the analysis of these endothelial antigens. In the present experiments we have attempted to characterize endothelial cell surface antigens...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2013.05.002
更新日期:2013-11-01 00:00:00
abstract::The 2',5'-oligoadenylate synthetase 1 (OAS1) is one of the major interferon-inducible proteins and a critical component of the host defense system against viral infection. A single nucleotide polymorphism (SNP), rs10774671, presumably responsible for alternate splicing of this gene, has frequently been associated with...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2012.11.011
更新日期:2013-03-01 00:00:00
abstract::The polymorphic nature of the KIR3DL1/S1 gene complex has been investigated through the development of a polymerase chain reaction-sequence-specific oligonucleotide probes (PCR-SSOP) allele typing system. The KIR3DL1/S1 system was applied to a healthy group of Northern Irish individuals to establish allele frequencies...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2004.03.003
更新日期:2004-06-01 00:00:00
abstract::The human TCRBV gene complex contains three BV12 genes and all are polymorphic. There are two alleles of BV12S4; one allele contains a stop codon in the coding region which precludes its expression. There are four alleles of BV12S2 and two alleles of BV12S3 genes; however, the substitutions present in both genes are c...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(94)00152-g
更新日期:1995-07-01 00:00:00
abstract::Accurate identification of HLA antibodies using the single antigen bead (SAB) assay is critical for assessment of pre/post-transplant immunological risk and successful virtual crossmatching. Unfortunately, high titer HLA antibodies can be missed or underestimated in the SAB assay as a result of interference with the d...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2019.04.019
更新日期:2019-07-01 00:00:00
abstract::The aim of this investigation was to evaluate the relationship between MHC alleles at the HLA-DRB1, DQB1 and TNFa microsatellite loci and levels of oral bacteria that play a role in the etiology of dental caries, and the DMFS index in 186 AA primparous women. The average age of the cohort was 20.8+/-3.7 years. The med...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(99)00088-9
更新日期:1999-10-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to determine whether the SUMO4 M55V (A163G) polymorphism confers susceptibility to type 1 diabetes (T1D). METHODS:A meta-analysis was conducted on the association between the SUMO4 M55V polymorphism and T1D using; (1) allelic contrast (G vs. A), and the (2) recessive (GG vs. GA+AA),...
journal_title:Human immunology
pub_type: 杂志文章,meta分析
doi:10.1016/j.humimm.2012.07.341
更新日期:2012-10-01 00:00:00
abstract::Alloantigen specific CD8+CD28- T suppressor (TS) cells differ from naturally occurring CD4+CD25+ T-regulatory (natural TR) cells not only by their phenotype but also by their mechanism of action. Natural TR have been extensively studied, leading to the identification of characteristic "molecular markers" such as Forkh...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2004.09.004
更新日期:2004-11-01 00:00:00
abstract::Human T suppressor cells (Ts), capable of preventing autologous T helper cells (Th) from reacting against xenogeneic pig endothelial cells and pig APC can be generated in vitro. Ts derive from a population of CD3(+)CD8(+)CD28(-) T lymphocytes and specifically recognize the MHC class I antigens of the APC used for in v...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(01)00238-5
更新日期:2001-05-01 00:00:00
abstract::To calculate reliable HLA gene and haplotype frequencies of bone marrow donors in various regions in the world, we have analyzed the HLA-A, -B, and -DR phenotype frequencies of 18 bone marrow donor registries with a total of more than 300,000 HLA-A, -B-typed donors. These registries were included in the 22nd edition o...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(96)00257-1
更新日期:1997-01-01 00:00:00
abstract::Human narcolepsy is almost exclusively associated with the major histocompatibility complex (MHC) class II antigen HLA-DR2 and is the strongest HLA-disease association described to date. Canine narcolepsy resembles the human disease in its behavioral manifestations and responses to therapeutic drugs. Therefore, mixed ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(89)90067-0
更新日期:1989-05-01 00:00:00
abstract::The production and serologic, as well as immunochemical properties of a cytotoxic murine IgG monoclonal antibody (Tü109) that precipitates HLA-class I molecules, are described. In the microcytotoxicity assay Tü109 supernatant was demonstrated on a panel of 424 HLA-ABC, -DR, -DQ, -MT typed normal Caucasian blood donors...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(85)90241-1
更新日期:1985-12-01 00:00:00
abstract::Pronase treatment is used in the flow cytometry crossmatch (FCXM) to prevent nonspecific antibody binding on B cells. However, we have observed unexpected positive results with pronase-treated T cells in human immunodeficiency virus (HIV)-infected patients. In this study, 25 HIV-infected patients without HLA antibodie...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2016.04.014
更新日期:2016-06-01 00:00:00
abstract::Human leukocyte antigen-G (HLA-G) is a nonclassical class Ib molecule belonging to the major histocompatibility complex. HLA-G appears to play a role in the suppression of immune responses and contribute to long-term immune escape or tolerance. The focus of this review is polymorphism in the HLA-G gene and protein and...
journal_title:Human immunology
pub_type: 杂志文章,评审
doi:10.1016/j.humimm.2009.07.015
更新日期:2009-12-01 00:00:00
abstract::Several complement regulatory proteins exist on self-cells to prevent damage by the serum complement system. In the present study, we aimed to perform quantitative analysis of membrane-bound complement regulators, CR1 (CD35), MCP (CD46), DAF (CD55), and MIRL (CD59), on peripheral blood neutrophils, monocytes, and lymp...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2013.01.011
更新日期:2013-05-01 00:00:00
abstract::Mannose-binding lectin (MBL) role in the carriage of oropharyngeal bacteria is not known. We investigated the association of smoking, MBL2 polymorphisms, and MBL concentrations with oropharyngeal carriage of respiratory bacteria in young men. Oropharyngeal specimens, MBL concentrations, and MBL2 gene polymorphisms wer...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2009.12.009
更新日期:2010-03-01 00:00:00
abstract::Interactions between human IgG with human FcgammaRI and FcgammaRIIa (R131) were studied to investigate the role of the hinge region of IgG3 and IgG1 in the binding of the antibodies to FcgammaR. It was found that a hinge deletion mutant of IgG3 (IgG3 m15) was reduced in its ability to bind to FcgammaRI and FcgammaRIIa...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(98)00075-5
更新日期:1998-11-01 00:00:00
abstract::To test the involvement of osteopontin gene (OPN) in systemic sclerosis (SSc) susceptibility, two OPN single nucleotide polymorphisms previously reported to be associated with systemic lupus erythematosus, namely -156G/GG (proximal promoter) and +1239A/C (3' untranslated region (UTR)), were tested in 357 Italian patie...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2011.06.009
更新日期:2011-10-01 00:00:00
abstract::Monoclonal antibodies have revealed complexity within the human leukocyte antigen class II antigens. We have studied epitopes present on a DR3 homozygous B-lymphoblastoid cell line using five polymorphic monoclonal antibodies produced and characterized in our laboratory. Serological analysis on a panel of B-cell lines...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(87)90107-8
更新日期:1987-11-01 00:00:00
abstract:HLA A:B:C:DRB1:DQB1 allele and haplotype frequencies were determined among India, Andhra Pradesh, Telugu speaking population from South India by Next Generation Sequencing. 180 bone marrow registry donors and 6 cord blood units from Jeevan Stem Cell Foundation (part of Be The Cure Registry), Chennai, Tamilnadu state we...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2018.05.005
更新日期:2018-08-01 00:00:00
abstract::There is a growing body of information about the soluble forms of HLA in serum but there are only a few reports discussing sHLA in other body fluids. We quantitated sHLA-I and sHLA-II concentrations in sweat, saliva and tear samples from five normal individuals with known HLA-phenotypes. We also studied sweat samples ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(98)00122-0
更新日期:1999-03-01 00:00:00
abstract::M proteins are coiled-coil dimers expressed on group A streptococcal cell surfaces. They have an important role in host antistreptococcal immunity and in poststreptococcal autoimmune sequelae. Controversy has arisen regarding whether type 5 M proteins are superantigenic for human T cells. To investigate this, we have ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/S0198-8859(97)00028-1
更新日期:1997-04-01 00:00:00
abstract::Human leukocyte antigen (HLA)-DR-positive synovial fibroblasts are frequently observed in rheumatoid arthritis (RA) and may be implicated in the autoimmune reaction because RA is associated with certain HLA-DRB1* alleles. The question of whether components of the class II antigen presentation pathway and specific DRB ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2004.09.005
更新日期:2004-12-01 00:00:00
abstract::Our objective was to study a possible contribution of MHC genes to S-HLA-I secretion in patients with Type I diabetes. Quantitatively, we used a highly sensitive enzyme-linked immunoassay to measure S-HLA-I in the serum of a total of 39 patients with Type I diabetes, as well as 36 kinships of 12 diabetic patients and ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(97)00096-7
更新日期:1997-07-01 00:00:00
abstract::Conclusions from association studies could be spurious because of population stratification; therefore we combined association with family studies seeking to confirm which human leukocyte antigen (HLA) class II alleles/haplotypes were associated with type 1 diabetes (T1D) in the admixed Latin America. By calculating t...
journal_title:Human immunology
pub_type: 杂志文章,meta分析
doi:10.1016/j.humimm.2011.03.012
更新日期:2011-07-01 00:00:00