当前位置： SCI文献检索 > HUMAN IMMUNOLOGY期刊下所有文献 > It's about time: The development and validation of a rapid optimized single antigen bead (ROB) assay protocol for LABScreen.

It's about time: The development and validation of a rapid optimized single antigen bead (ROB) assay protocol for LABScreen.

Abstract：

:The LABScreen single antigen bead assay (SAB) is a method widely used for the identification and monitoring of human leukocyte antigen (HLA) antibodies in patients pre-and post-transplant. While accurate testing of patient samples is key for optimal patient care, time can also be important, especially during deceased donor workups or post-transplant assessments. Here we describe the development and validation of the Rapid Optimized SAB (ROB) protocol, a modified version of the One Lambda LABScreen SAB (OLSAB) procedure, which reduces assay time from 85 to 25min (>70% reduction) without impacting assay quality or sensitivity. Optimization steps included shortened centrifugation cycles and reduced serum and secondary antibody incubation times in combination with increased secondary antibody concentration. Linear regression analysis of baseline median fluorescence intensity (MFI) values showed excellent correlation between the ROB and OLSAB protocols (r2>0.98) for both class I and class II antibodies in 58 sera tested in two HLA laboratories. Importantly, the ROB protocol demonstrated a trend towards improved inter-laboratory MFI concordance when compared to the OLSAB procedure (r2=0.9816 vs 0.9451), especially for HLA antibody specificities in the 500-2000 MFI range (r2=0.7824 vs 0.6313). Implementation of the ROB protocol will expedite HLA antibody testing and may improve reproducibility of the SAB assay.

journal_name

Hum Immunol

journal_title

Human immunology

authors

Liwski RS,Greenshields AL,Murphey C,Bray RA,Gebel HM

doi

10.1016/j.humimm.2017.05.001

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

489-499

issue

7-8

eissn

0198-8859

issn

1879-1166

pii

S0198-8859(17)30073-3

journal_volume

78

pub_type

杂志文章

相关文献

HUMAN IMMUNOLOGY文献大全
  • Production of a chimeric allergen derived from the major allergen group 1 of house dust mite species in Nicotiana benthamiana.

    abstract：:Plants are widely accepted as a general platform for the large-scale production of recombinant proteins, which has been demonstrated by the successful expression of various exogenous proteins. Using plants as a bioreactor for mass production of target proteins for vaccines is thought to show the most potential. This s...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2013.01.002

    authors： Li C,Jiang Y,Guo W,Liu Z

    更新日期：2013-05-01 00:00:00

  • TGF-β1-induced regulatory T cells.

    abstract：:Besides central tolerance peripheral tolerance is an important mechanism to avoid development of autoimmunity. Naturally occurring thymic-derived regulatory T cells (nTreg) mediate peripheral tolerance by suppressing autoreactive T cells clones having escaped thymic deletional control. This implies that nTreg have the...

    journal_title：Human immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.humimm.2015.06.015

    authors： Hadaschik EN,Enk AH

    更新日期：2015-08-01 00:00:00

  • Uniparental maternal disomy 6 in a renal transplant patient.

    abstract：:HLA analysis of the family of a renal transplant patient revealed an extremely rare condition. On repeated typings the only demonstrable HLA antigens shown in the propositus were from the maternal haplotype, HLA-A11,-B46,-CW1,-DR14,-DQ1. No paternal antigens could be demonstrated either by serologic or by DNA-typing m...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(95)00148-4

    authors： van den Berg-Loonen EM,Savelkoul P,van Hooff H,van Eede P,Riesewijk A,Geraedts J

    更新日期：1996-01-01 00:00:00

  • Di-allelic alloantigenic systems on subsets of T cells.

    abstract：:Sera obtained from multiparous women and some of other origin contain antibodies which react with antigens on T cell subsets. These antibodies recognize two distinct diallelic systems, one of which is mainly present on T gamma cells while the other is present on T mu cells. The sera that reacted with the T gamma cells...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(82)90011-8

    authors： van Leeuwen A,Festenstein H,van Rood JJ

    更新日期：1982-04-01 00:00:00

  • Prediction of spurious HLA class II typing results using probabilistic classification.

    abstract：:While modern high-throughput sequence-based HLA genotyping methods generally provide highly accurate typing results, artefacts may nonetheless arise for numerous reasons, such as sample contamination, sequencing errors, read misalignments, or PCR amplification biases. To help detecting spurious typing results, we test...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2016.01.012

    authors： Schöfl G,Schmidt AH,Lange V

    更新日期：2016-03-01 00:00:00

  • Genetic diversity of HLA system in a population from Guerrero, Mexico.

    abstract：:We studied HLA class I (HLA-A, -B) and class II (HLA-DRB1, -DQB1) alleles by PCR-SSP based typing in 144 Mexicans from the state of Guerrero to obtain information regarding allelic and haplotypic frequencies. We find that the ten most frequent haplotypes in the state of Guerrero include eight Native American and two E...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2019.05.015

    authors： Juárez-Nicolás F,Barquera R,Martínez-Álvarez JC,Hernández-Zaragoza DI,Ortega-Yáñez A,Arrieta-Bolaños E,Clayton S,Bravo-Acevedo A,Arrazola-García MA,Immel A,Juárez-Barreto V,Benítez-Arvizu G,Vega-Martínez MDR,García-Álvarez R,

    更新日期：2020-09-01 00:00:00

  • Anti-HLA antibodies interfere in the detection of soluble HLA class I molecules.

    abstract：:Heart transplant rejection is routinely defined by histological evaluation of endomyocardial biopsies (EMB). As elevated levels of donor derived sHLA (dsHLA) can be detected in the serum of transplanted patients just before or during rejection, quantification of donor specific soluble counterparts of HLA Class I (sHLA...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/s0198-8859(99)00010-5

    authors： Koelman CA,Ensink W,Mulder A,Tanke J,Doxiadis II,Claas FH

    更新日期：1999-05-01 00:00:00

  • Killer cell immunoglobulin-like receptor genotypes in a Chilean population from Talca.

    abstract：:Ninety healthy and unrelated volunteers were randomly selected to study the gene frequencies of Killer-cell immunoglobulin-like receptors (KIRs) in a Chilean (Talca) population. KIR typing was resolved by PCR-single specific primer (SSP), and their gene frequencies were calculated by direct counting of the number of p...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2018.06.007

    authors： Castro-Santos P,Verdugo RA,Díaz-Peña R

    更新日期：2018-09-01 00:00:00

  • HLA-DQ beta 3.1 allele is a determinant of susceptibility to DR4-associated rheumatoid arthritis.

    abstract：:Rheumatoid arthritis is associated with HLA-DR4 in several ethnic groups. Since DR4 haplotypes encode a diverse array of class II molecules, it is of interest to characterize the nature of the primary association. We have examined molecular polymorphisms of HLA class II gene products expressed by normals and rheumatoi...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(89)90033-5

    authors： Lanchbury JS,Sakkas LI,Marsh SG,Bodmer JG,Welsh KI,Panayi GS

    更新日期：1989-09-01 00:00:00

  • The association of class I HLA alleles and antibody levels after a single dose of measles vaccine.

    abstract：:Despite the success of the current measles vaccine in controlling disease in industrialized countries, the importance of vaccine failure has become increasingly apparent. Our objective was to determine if associations exist between seronegativity after measles vaccination and class I human leukocyte antigen (HLA) alle...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/s0198-8859(02)00741-3

    authors： Jacobson RM,Poland GA,Vierkant RA,Pankratz VS,Schaid DJ,Jacobsen SJ,Sauver JS,Moore SB

    更新日期：2003-01-01 00:00:00

  • Defective expression and function of the ILT2/CD85j regulatory receptor in dendritic cells from patients with systemic lupus erythematosus.

    abstract：:Dendritic cells (DCs) play an important role in inducing immune tolerance. Inhibitory receptors, such as ILT2/CD85j, are involved in the tolerogenic effect of DCs and previous studies have indicated the important role of these receptors on the pathogenesis of autoimmune diseases. The aim of this study was to evaluate ...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2013.05.006

    authors： Monsiváis-Urenda A,Gómez-Martin D,Santana-de-Anda K,Cruz-Martínez J,Alcocer-Varela J,González-Amaro R

    更新日期：2013-09-01 00:00:00

  • In vitro model for the activation of CD4 and CD8 T cell receptors.

    abstract：:Previously, most models that sought to explain the misregulation of immune cell function assumed molecular similarities between the disease-causing pathogens and the host's proteins. In recent time several different models have been proposed and in this study, these concepts are compared to a new hypothesis proposing ...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2009.05.010

    authors： Arneth BM

    更新日期：2009-09-01 00:00:00

  • The association of de novo anti-HLA-DPB1 donor-specific antibody formation and primary graft failure after allogeneic hematopoietic cell transplantation.

    abstract：:Despite advances in HLA matching, graft failure remains a serious complication after allogeneic hematopoietic cell transplantation (HCT). Pre-formed anti-HLA donor-specific antibodies (DSAs) have been associated with graft failure, but the impact of newly developing (de novo) anti-HLA antibodies on HCT outcomes remain...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2018.08.009

    authors： Hefazi M,Hogan WJ,Wakefield LL,Gandhi MJ

    更新日期：2018-12-01 00:00:00

  • Developmental origin of pre-DC2.

    abstract：:It is generally accepted that dendritic cells can be generated from either myeloid or lymphoid derived progenitors. Ample information has been collected on the development and nature of myeloid DC type 1 (DC1). In contrast, our current understanding on the origin and function of the lymphoid derived DC type 2 (DC2) is...

    journal_title：Human immunology

    pub_type： 杂志文章,评审

    doi：10.1016/s0198-8859(02)00745-0

    authors： Blom B,Ligthart SJ,Schotte R,Spits H

    更新日期：2002-12-01 00:00:00

  • Kinetics of the pregnancy-induced humoral and cellular immune response against the paternal HLA class I antigens of the child.

    abstract：:Pregnancy can prime the maternal humoral immune response against paternal human leukocyte antigens (HLA) of the child. Previous studies have reported that formation of antibodies against inherited paternal HLA is associated with the presence of primed cytotoxic T lymphocytes (CTLs) specific for these antigens. Recentl...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/s0198-8859(02)00396-8

    authors： van Kampen CA,Versteeg-vd Voort Maarschalk MF,Langerak-Langerak J,Roelen DL,Claas FH

    更新日期：2002-06-01 00:00:00

  • Increased RUNX1 expression in patients with immune thrombocytopenia.

    abstract：:Immune thrombocytopenia (ITP) is a heterogeneous autoimmune disease, characterized by dysregulation of cellular immunity. Th17 and associated IL-17 were involved in the pathogenesis of ITP. Runt-related transcription factor 1 (RUNX1), a member of the runt domain-containing family of transcription factors, is required ...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2016.06.004

    authors： Zhong X,Wu Y,Liu Y,Zhu F,Li X,Li D,Li Z,Zeng L,Qiao J,Chen X,Xu K

    更新日期：2016-08-01 00:00:00

  • Expression of the costimulator molecules, CD80, CD86, CD28, and CD152 on lymphocytes from neonates and young children.

    abstract：:The expression of CD80, CD86, CD28, and CD152 were examined on peripheral blood lymphocytes from adults, neonates (cord blood lymphocytes) and young children (2-20 months of age). There was no difference in the expression of CD80 or CD86 between adult and neonatal B cells, either resting or activated. A higher percent...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/s0198-8859(99)00090-7

    authors： Elliott SR,Macardle PJ,Roberton DM,Zola H

    更新日期：1999-11-01 00:00:00

  • Expression of human leukocyte antigen-G in systemic lupus erythematosus.

    abstract：:The purpose of this study was to examine the expression of human leukocyte antigen-G (HLA-G) in patients with systemic lupus erythematosus (SLE) and its relation with interleukin-10 (IL-10) production. The study included 50 female SLE patients and 59 healthy female donors. HLA-G expression in peripheral blood and cuta...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2007.11.001

    authors： Rosado S,Perez-Chacon G,Mellor-Pita S,Sanchez-Vegazo I,Bellas-Menendez C,Citores MJ,Losada-Fernandez I,Martin-Donaire T,Rebolleda N,Perez-Aciego P

    更新日期：2008-01-01 00:00:00

  • Complex associations between HLA-DRB1 genes and female rheumatoid arthritis: results from a prospective study.

    abstract：:We followed 138 Spanish patients (37 men and 101 women) with rheumatoid arthritis (RA) to analyze whether patient sex influenced the HLA-DRB1 associations with disease susceptibility. Results showed that, although a high increase of the shared epitope (SE) was observed in both genders, distribution of HLA-DRB1 specifi...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/s0198-8859(99)00119-6

    authors： Gonzalez-Escribano MF,Rodriguez R,Valenzuela A,Garcia A,Núñez-Roldan A

    更新日期：1999-12-01 00:00:00

  • Sensitization of human lymphocytes in vitro. Kinetics and specificity of the response to hemocyanins.

    abstract：:In vitro primary sensitization of human peripheral blood T-cells to hemocyanins was detected during a 12-14 day culture period with antigen (KLH or HCH)-pulsed macrophages. Primed T-cells proliferate in secondary culture (2-3 days) when restimulated with antigen presented by macrophages. The kinetics of primary sensit...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(82)90035-0

    authors： Ford D,Hamblin A,Dumonde D,Burger D

    更新日期：1982-06-01 00:00:00

  • The relationship between HLA class II polymorphisms and somatic deletions in testicular B cell lymphomas of Dutch patients.

    abstract：:Several risk factors including immune deficiencies, infections, and autoimmune diseases have been established for non-Hodgkin's lymphoma (NHL). For diffuse large B cell lymphoma (DLBCL), the most common type of lymphoma, no risk factors have been described, which may be due to the intrinsic heterogeneity of this disor...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2006.03.019

    authors： Riemersma SA,Jordanova ES,Haasnoot GW,Drabbels J,Schuuring E,Schreuder GM,Kluin PM

    更新日期：2006-04-01 00:00:00

  • Anti-HLA-B7,B27,Bw42,Bw54,Bw55,Bw56, Bw67,Bw73 monoclonal antibodies: specificity, idiotypes, and application for a double determinant immunoassay.

    abstract：:The monoclonal antibodies (MoAbs) KS3 and KS4 are secreted by hybridomas constructed with splenocytes from a BALB/c mouse sequentially immunized with the cultured lymphoid cells JKu and LG-2 which share only the HLA-B27 specificity. Serologic and immunochemical assays have shown that the two MoAbs recognize the same (...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(88)90071-7

    authors： Sakaguchi K,Ono R,Tsujisaki M,Richiardi P,Carbonara A,Park MS,Tonai R,Terasaki PI,Ferrone S

    更新日期：1988-03-01 00:00:00

  • Genetic polymorphisms of interleukin-6 modulate fibrosis progression in mild chronic hepatitis C.

    abstract：:Genetic polymorphisms of interleukin-6 (IL-6) (-1363G>T, -597G>A, -572G>C, -174G>C, +2954G>C) may affect the outcomes of several diseases. This study was aimed to verify the role of these polymorphisms on the disease progression of patients with hepatitis C virus (HCV) infection and persistently normal transaminases (...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2010.06.006

    authors： Falleti E,Fabris C,Vandelli C,Colletta C,Cussigh A,Smirne C,Fontanini E,Cmet S,Minisini R,Bitetto D,Toniutto P,Pirisi M

    更新日期：2010-10-01 00:00:00

  • Use of a murine T-cell hybridoma expressing human T-cell receptor alpha- and beta-gene products as a tool for the production of human T-cell receptor-specific monoclonal antibodies.

    abstract：:We describe the production of mouse monoclonal antibodies specific for the human TcR using as the immunogen transfected murine T-cell hybridoma cells coexpressing mouse CD3 with human Jurkat TcR alpha and beta chains. The shortage of monoclonal antibodies (mAbs) specific for the human TcR-V alpha and V beta families r...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(92)90098-8

    authors： Zumla A,McCormack A,George A,Batchelor R,Lechler R

    更新日期：1992-11-01 00:00:00

  • CD3+CD20+ T cells and their roles in human diseases.

    abstract：:CD3+CD20+ T cells are a population of CD3+ T cells co-expressing CD20 that make up to ∼3-5% of the CD3+ T-cell compartment in the peripheral blood of human beings. In healthy individuals, CD3+CD20+ T cells are heterogeneous for containing a lower proportion of CD4+ cells, but produce higher levels of IL-17A and/or IFN...

    journal_title：Human immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.humimm.2019.01.001

    authors： Chen Q,Yuan S,Sun H,Peng L

    更新日期：2019-03-01 00:00:00

  • HLA class II molecules transduce accessory signals affecting the CD3 but not the interleukin-2 activation pathway in T blasts.

    abstract：:MHC class II molecules play a central role in the control of the immune response, but their biologic function and mechanism of action on the surface of activated human T lymphocytes are not entirely understood. In our study, the functional role of HLA class II molecules in T-blast proliferation was investigated by ana...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(93)90552-c

    authors： Di Rosa F,D'Oro U,Ruggiero G,Racioppi L,Acquaviva A,Ferrone S,Fontana S,Zappacosta S

    更新日期：1993-12-01 00:00:00

  • Association between the SUMO4 M55V (A163G) polymorphism and susceptibility to type 1 diabetes: a meta-analysis.

    abstract：OBJECTIVE:The aim of this study was to determine whether the SUMO4 M55V (A163G) polymorphism confers susceptibility to type 1 diabetes (T1D). METHODS:A meta-analysis was conducted on the association between the SUMO4 M55V polymorphism and T1D using; (1) allelic contrast (G vs. A), and the (2) recessive (GG vs. GA+AA),...

    journal_title：Human immunology

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.humimm.2012.07.341

    authors： Song GG,Choi SJ,Ji JD,Lee YH

    更新日期：2012-10-01 00:00:00

  • CR1 levels and gene polymorphisms exhibit differential association with falciparum malaria in regions of varying disease endemicity.

    abstract：:Complement receptor 1 (CR1/CD35) levels on erythrocytes and related CR1 polymorphisms have been associated with response to falciparum malaria in populations inhabiting malaria-endemic regions. Differences in disease association profiles of its low expression alleles have been observed in populations from different re...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2009.02.001

    authors： Sinha S,Jha GN,Anand P,Qidwai T,Pati SS,Mohanty S,Mishra SK,Tyagi PK,Sharma SK,Venkatesh V,Habib S

    更新日期：2009-04-01 00:00:00

  • Detection of HLA restricted anti-minor histocompatibility antigen(s) reactive cells from skin GVHD lesions.

    abstract：:Recipients of marrow grafts from HLA-identical, (including MLC non-reactive) sibling donors have a 40-50% incidence of acute graft vs. host disease (GVHD); the involvement of non-HLA linked, minor histocompatibility antigens (miHA) has been implicated. One of the target tissues of GVHD is the skin where morphological ...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(84)90064-8

    authors： Reinsmoen NL,Kersey JH,Bach FH

    更新日期：1984-12-01 00:00:00

  • HLA in a cohort of Brazilian patients with sarcoidosis.

    abstract：:Sarcoidosis is a multisystem granulomatous disorder of unknown etiology. Hereditary etiology has been proposed as a cause of the sarcoidosis, and some Human Leucocyte Antigen (HLA) alleles have been related to the diagnosis or severity of the disease. Löfgren's syndrome has been linked to patients with the DRB1*03 all...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2013.07.009

    authors： da Costa CH,Silva VL,Fabricio-Silva GM,Usnayo M,Rufino R,Porto LC

    更新日期：2013-10-01 00:00:00