Real-time PCR using fluorescent resonance emission transfer probes for HLA-B typing.


:HLA genotyping by polymerase chain reaction (PCR) has some inherent labor-intensive and effort-demanding limitations. To overcome them, we have developed a real-time PCR with hybridization probes approach able to obtain a medium-low resolution HLA-B genotyping with fewer tubes and probes and with a shorter time requirement. Our strategy used 18 simultaneous reactions amplifying HLA-B alleles and an internal control. Monitorization of both amplifications in each tube is performed by the simultaneous application of two fluorescent resonance emission transfer probes: the first probe, different for each tube, is specific for the HLA-B locus and the second probe detects the control gene. A medium-low resolution (300 HLA-B allelic groups) typing is obtained for each sample by analyzing the melting curve patterns. Because some alleles may be determined without using the complete set of reactions, we present an alternative strategy: a first round of seven reactions and, according to the result, a second (or third) round of PCRs to solve the ambiguities. This method was validated in pretyped clinical samples and the results were completely concordant. Moreover, fewer ambiguous results were obtained. In summary, we present a new, faster, and more accurate method than currently used PCR techniques to type HLA-B alleles.


Hum Immunol


Human immunology


Faner R,Casamitjana N,Coll J,Caro P,Pujol-Borrell R,Palou E,Juan M




Has Abstract


2006-04-01 00:00:00














  • Soluble HLA proteins with bound peptides are released from the cell surface by the membrane metalloproteinase.

    abstract::The metalloproteinase (MPase)-mediated pathway of MHC class I processing is a distinct cellular mechanism that generates soluble HLA proteins. It has been implicated in modulation of immune responses induced during transplantation events. It is, therefore, important to define the characteristics of soluble HLA species...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Demaria S,Bushkin Y

    更新日期:2000-12-01 00:00:00

  • The role of human leukocyte antigen DRB1-DQB1 haplotypes in the susceptibility to acquired idiopathic thrombotic thrombocytopenic purpura.

    abstract::The acquired form of idiopathic thrombotic thrombocytopenic purpura (TTP) is an autoimmune disease, in which the underlying ADAMTS13-deficiency is caused by inhibitory autoantibodies against the protease. Human leukocyte antigens (HLA), responsible for antigen presentation, play an important role in the development of...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Sinkovits G,Szilágyi Á,Farkas P,Inotai D,Szilvási A,Tordai A,Rázsó K,Réti M,Prohászka Z

    更新日期:2017-02-01 00:00:00

  • Typing for human alloantigens with the primed lymphocyte typing (PLT) technique with notes on the interpretation of PLT data.

    abstract::A typing system for HLA-D/DR-associated PLT-defined determinants, which have been called "DP" antigens, is reported. Some of the results concerning a data interpretation system, the reproducibility of PLT results, and the correlations between the results of HLA-D, -DR, and DP typing are presented. Also, a "new" human ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Morling N,Jakobsen BK,Platz P,Ryder LP,Svejgaard A,Thomsen M

    更新日期:1981-07-01 00:00:00

  • TCR repertoire of suppressor CD8+CD28- T cell populations.

    abstract::The cellular basis of graft rejection and the development of strategies for specific suppression of T cell responses against allogeneic and xenogeneic transplants represents an area of active investigation. Recently, a population of MHC-class I restricted CD8+CD28- T suppressor cells (Ts) which are able to inhibit spe...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Pennesi G,Liu Z,Ciubotariu R,Jiang S,Colovai A,Cortesini R,Suciu-Foca N,Harris P

    更新日期:1999-04-01 00:00:00

  • Four tumor necrosis factor alpha genes polymorphisms and periodontitis risk in a Chinese population.

    abstract:BACKGROUND:Tumour necrosis factor-alpha (TNF-alpha) has been implicated in the pathogenesis of generalized aggressive periodontitis (AgP) and chronic periodontitis (CP). The objective of the present study was to evaluate the association of four TNF-alpha gene polymorphisms (-1031T/C, -857C/T, -308G/A and -238G/A) with ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Yang W,Jia Y,Wu H

    更新日期:2013-12-01 00:00:00

  • Effect of delayed graft function in hypersensitized kidney transplant recipients.

    abstract::There is increased evidence about the deleterious effect of delayed graft function (DGF) in both short- and long-term kidney graft outcome. Among the mechanisms involved in the production of DGF, immune factors play a role, especially in the level of hypersensitization. From the 1389 patients transplanted at our hospi...

    journal_title:Human immunology

    pub_type: 杂志文章,评审


    authors: López-Hoyos M,Fernández-Fresnedo G,Rodrigo E,Ruiz JC,Arias M

    更新日期:2005-04-01 00:00:00

  • HLA class II molecules transduce accessory signals affecting the CD3 but not the interleukin-2 activation pathway in T blasts.

    abstract::MHC class II molecules play a central role in the control of the immune response, but their biologic function and mechanism of action on the surface of activated human T lymphocytes are not entirely understood. In our study, the functional role of HLA class II molecules in T-blast proliferation was investigated by ana...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Di Rosa F,D'Oro U,Ruggiero G,Racioppi L,Acquaviva A,Ferrone S,Fontana S,Zappacosta S

    更新日期:1993-12-01 00:00:00

  • A collaborative European search for non-DQA1*05-DQB1*02 celiac disease loci on HLA-DR3 haplotypes: analysis of transmission from homozygous parents.

    abstract::The HLA-DQA1*05 with DQB1*02 alleles are a major risk factor for celiac disease (CD). To search for additional human leukocyte antigen (HLA) risk factors, we looked on the DR3-DQ2 risk haplotype, selected because it carries both DQ risk alleles in cis and is the more represented among CD patients. In a European consor...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Louka AS,Moodie SJ,Karell K,Bolognesi E,Ascher H,Greco L,Momigliano-Richiardi P,Partanen J,Ciclitira PJ,Sollid LM,European Genetics Cluster on Celiac Disease.

    更新日期:2003-03-01 00:00:00

  • Association of TNF -1031 C/C as a potential protection marker for leprosy development in Amazonas state patients, Brazil.

    abstract::Polymorphisms present in the TNF promoter region has shown to influence the gene transcription. Leprosy displays different clinical manifestations according to the immune responses of the individual infected with Mycobacterium leprae. In this study, we evaluated the single nucleotide polymorphisms (SNPs) -238 G/A (rs3...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Silva GA,Ramasawmy R,Boechat AL,Morais AC,Carvalho BK,Sousa KB,Souza VC,Cunha MG,Barletta-Naveca RH,Santos MP,Naveca FG

    更新日期:2015-03-01 00:00:00

  • Potential influence of interleukin-1 haplotype IL-1 beta-511*T-IL-1RN*1 in conferring low risk to middle third location of esophageal cancer: a case-control study.

    abstract::Interleukin (IL)-1 gene polymorphisms affect several inflammatory diseases, including cancer. Therefore, we studied genetic association of biallelic (-511C>T) polymorphism of IL-1 beta and 86-bp VNTR polymorphism of IL-1RN in 159 patients with esophageal cancer (EC) and 194 age- and gender-matched healthy controls. Ge...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Upadhyay R,Jain M,Kumar S,Ghoshal UC,Mittal B

    更新日期:2008-03-01 00:00:00

  • Expression of extra class I major histocompatibility antigens on T-cell acute lymphoblastic leukemia (ALL) lymphoblasts.

    abstract::The presence of extra HLA antigens has been demonstrated, serologically and biochemically, on the surface of lymphoblasts from a patient with acute lymphoblastic leukemia of the T-cell subtype (T-ALL). Family analysis of this patient revealed the presence of the expected antigens, plus an additional HLA antigen (A24) ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Orgad S,Yang SY,Gazit E,Relias V,Zaizov R,Lyson S,Yunis EJ

    更新日期:1985-03-01 00:00:00

  • B cells in chronic graft-versus-host disease.

    abstract::Allogeneic hematopoietic stem cell transplantation (alloHCT) is the definitive therapy for numerous otherwise incurable hematologic malignancies and non-malignant diseases. The genetic disparity between donor and recipient both underpins therapeutic effects and confers donor immune system-mediated damage in the recipi...

    journal_title:Human immunology

    pub_type: 杂志文章,评审


    authors: McManigle W,Youssef A,Sarantopoulos S

    更新日期:2019-06-01 00:00:00

  • The genetic structure of Mexican Mestizos of different locations: tracking back their origins through MHC genes, blood group systems, and microsatellites.

    abstract::Mexican Mestizos, who are the result of the admixture of Spanish, Indian, and Black genes, were analyzed for different systems. Three populations from geographical distinct areas were studied: the north (State of Nuevo Leon ), the center (State of Guanajuato), and the highlands (mainly Mexico City). Ten blood group sy...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Gorodezky C,Alaez C,Vázquez-García MN,de la Rosa G,Infante E,Balladares S,Toribio R,Pérez-Luque E,Muñoz L

    更新日期:2001-09-01 00:00:00

  • Lack of an association between interleukin-6 gene promoter polymorphisms (-174G/C, -572G/C) and ischemic heart disease and/or ischemic stroke: a meta-analysis.

    abstract::The association between single-nucleotide polymorphisms -174G/C (rs1800795) and -572G/C (rs1800796) in the interleukin-6 (IL-6) gene promoter region and ischemic heart disease (IHD)/ischemic stroke (IS) remains controversial and ambiguous. In this study, we performed a more precise estimation of the relationship by a ...

    journal_title:Human immunology

    pub_type: 杂志文章,meta分析


    authors: Ma Y,Tang RK,Yang X,Peng GG,Liu Y,Wang XM,Wu BH,Yu JM

    更新日期:2011-08-01 00:00:00

  • Frequency of alloantibodies reacting with PHA-activated T lymphocytes, unexplainable by known HLA activities.

    abstract::Two hundred and three sera obtained from multiparous women were screened to determine the frequency of the appearance of antibodies reactive with PHA-activated T lymphocytes, but not with resting B or T cells from the same donor. The panel of cells on which the sera were tested consisted of lymphocytes from 38 unrelat...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Kim SJ,Christiansen FT,Gosar I,Silver DM,Pollack MS,Dupont B

    更新日期:1980-12-01 00:00:00

  • HLA study in Bolivian Quechua Amerindians from Titikaka Lake Area.

    abstract::Quechua Amerindians established Inca Empire and chose Cuzco as their capital. Their language is closely related to that of Aymara ethnic group and both of them were originated from Titikaka Lake Altiplano area. In the present study we have analyzed Bolivian Quechua HLA profile and found that it has common characters w...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Arnaiz-Villena A,Juarez I,Lopez-Nares A,Crespo-Yuste E,Callado A,Suarez-Trujillo F

    更新日期:2020-07-01 00:00:00

  • Propagation of infiltrating lymphocytes and graft coronary disease in cardiac transplant recipients.

    abstract::The pattern of lymphocyte growth from endomyocardial biopsies in 55 heart transplant recipients was shown to be correlated with the subsequent development of graft coronary disease. Persistent lymphocyte growth was observed in 39 patients, and 15 of these growers (or 41%) developed graft coronary disease. In contrast,...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Kaufman CL,Zeevi A,Kormos RL,Zerbe TR,Keenan RJ,Uretsky BF,Griffith BP,Hardesty RL,Duquesnoy RJ

    更新日期:1990-06-01 00:00:00

  • 4-Locus high-resolution HLA allele and haplotype frequencies in admixed population from Nicaragua.

    abstract::A total of 155 Nicaraguan Mestizos from across the country were genotyped at high-resolution for the human leukocyte antigen loci HLA-A, -B, -C, and -DRB1 using sequence-based typing methods. The respective allele and extended haplotype frequencies, as well as Hardy-Weinberg proportions were calculated. The most frequ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Arrieta-Bolaños E,Madrigal-Sánchez JJ,Stein JE,Moreira-Espinoza MJ,Paredes-Carias E,Vanegas-Padilla Y,Salazar-Sánchez L,Madrigal JA,Marsh SGE,Shaw BE

    更新日期:2019-07-01 00:00:00

  • Developmental origin of pre-DC2.

    abstract::It is generally accepted that dendritic cells can be generated from either myeloid or lymphoid derived progenitors. Ample information has been collected on the development and nature of myeloid DC type 1 (DC1). In contrast, our current understanding on the origin and function of the lymphoid derived DC type 2 (DC2) is...

    journal_title:Human immunology

    pub_type: 杂志文章,评审


    authors: Blom B,Ligthart SJ,Schotte R,Spits H

    更新日期:2002-12-01 00:00:00

  • Monoclonal and xenoantibodies specific for HLA-DR inhibit primary responses to HLA-D but fail to inhibit secondary proliferative (PLT) responses to allogeneic cells.

    abstract::Monoclonal antibodies (DA-2 and CA-2) and xenoantisera (rabbit anti-human, p23,30) specific for HLA-DR framework determinants were added to primary and secondary mixed lymphocyte cultures. Although such antisera were shown to inhibit primary MLC, primed lymphocytes were much less sensitive to the blocking effects of t...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Eckels DD,Woody JN,Hartzman RJ

    更新日期:1981-10-01 00:00:00

  • Characteristics of the cytosol-synthesized proteins generating class-I-bound peptides.

    abstract::The proteins synthesized in the cytosol are several thousand, and the number of peptides potentially able to be bound by class I molecules they can generate is therefore huge. On the other hand, the actual number of peptide-class I complexes required for CTL activation is around 200. We focused on the peptides bound b...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Frumento G,Molina F,Pernis B,Ferrara GB

    更新日期:1994-09-01 00:00:00

  • Identification of a microRNA signature in dendritic cell vaccines for cancer immunotherapy.

    abstract::Dendritic cells (DCs) exposed to tumor antigens followed by treatment with T(h)1-polarizing differentiation signals have paved the way for the development of DC-based cancer vaccines. Critical parameters for assessment of the optimal functional state of DCs and prediction of the vaccine potency of activated DCs have i...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Holmstrøm K,Pedersen AW,Claesson MH,Zocca MB,Jensen SS

    更新日期:2010-01-01 00:00:00

  • Orientation of HLA-DNA gene and identification of a CpG island-associated gene adjacent to DNA in human major histocompatibility complex class II region.

    abstract::We have constructed the detailed physical map of the HLA class II gene region by the pulsed field gel electrophoresis (PFGE) and cosmid walking technique. In this process, the DNA gene was found to be located telomeric to DPA1 with the 5'----3' orientation which is the same as the DPA1 and DPA2 genes, but opposite to ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Okamoto N,Ando A,Kawai J,Yoshiwara T,Tsuji K,Inoko H

    更新日期:1991-11-01 00:00:00

  • Human leukocyte antigen-G polymorphism in relation to expression, function, and disease.

    abstract::Human leukocyte antigen-G (HLA-G) is a nonclassical class Ib molecule belonging to the major histocompatibility complex. HLA-G appears to play a role in the suppression of immune responses and contribute to long-term immune escape or tolerance. The focus of this review is polymorphism in the HLA-G gene and protein and...

    journal_title:Human immunology

    pub_type: 杂志文章,评审


    authors: Larsen MH,Hviid TV

    更新日期:2009-12-01 00:00:00

  • Human T4+ T-lymphocyte clones specific for the B fragment of tetanus toxin.

    abstract::Two T4+ cloned T-lymphocyte lines specific for a papain digest product of tetanus toxin are functionally characterized. The two clones were obtained from peripheral blood mononuclear cells activated in vitro by tetanus toxoid, expanded with IL-2, and cloned in soft agar. Both clones could be induced to undergo blastog...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Scrivner D,Kristof S,Rodey G

    更新日期:1987-08-01 00:00:00

  • Serologic recognition of a HLA-B44(12) subgroup associated with the 44.2 specificity defined by CML.

    abstract::Several investigators have reported that subdivisions of HLA-B44, which are not defined by available cytotoxic antisera, can be detected by cytotoxic T lymphocytes. The present study was designed to evaluate two sera which appear to recognize a specificity on some HLA-B44 and B45 positive cells and to determine whethe...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Heise ER,Tekolf WA,Hampton JP

    更新日期:1984-11-01 00:00:00

  • Cell culture density modulates the incorporation of HLA antigens by enveloped viruses.

    abstract::Uninfected, as well as feline leukemia virus (FeLV) infected human cells cultured under high cell density conditions undergo changes in the expression of major histocompatibility complex (MHC) antigens, as determined by indirect trace binding radio immunoassay (RIA) using monoclonal anti-HLA antibodies and by decrease...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Azocar J,Essex M,Yunis EJ

    更新日期:1983-06-01 00:00:00

  • Bone marrow transplantation in Brazil.

    abstract::The first program of bone marrow transplantation in Latin America was started 5 years ago at the Federal University Hospital, Curitiba, Paraná. The results of 62 patients who underwent bone marrow transplantation are presented and discussed. ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Ferreira E,Dulley FL,Morsoletto F,Neto JZ,Pasquini R

    更新日期:1985-11-01 00:00:00

  • A high degree of HLA disparity arises from limited allelic diversity: analysis of 1775 unrelated bone marrow transplant donor-recipient pairs.

    abstract::The allelic diversity and associated human leukocyte antigen (HLA) disparity of 1775 bone marrow recipients and their unrelated donors, matched for six of six (1361/1775,77%), five of six (397/1775, 22%), or four of six (17/1775, 1%) HLA-A, -B, -DR antigens, were retrospectively evaluated. The comprehensive HLA analys...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Hurley CK,Fernandez-Vina M,Hildebrand WH,Noreen HJ,Trachtenberg E,Williams TM,Baxter-Lowe LA,Begovich AB,Petersdorf E,Selvakumar A,Stastny P,Hegland J,Hartzman RJ,Carston M,Gandham S,Kollman C,Nelson G,Spellman S,Sett

    更新日期:2007-01-01 00:00:00

  • Alloantibodies in heart transplantation.

    abstract::The presence of complement fixing anti-human leukocyte antigen (HLA) antibodies in the circulation of organ transplant recipients may result in heart allograft rejection. Here, we assessed the clinical impact of pre- and post-transplantation allosensitization on long-term survival of heart allografts. Sequential sampl...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Ho EK,Vlad G,Colovai AI,Vasilescu ER,Schwartz J,Sondermeijer H,Burke E,Marboe CC,Itescu S,Suciu-Foca N,Mancini D

    更新日期:2009-10-01 00:00:00