Identification of a microRNA signature in dendritic cell vaccines for cancer immunotherapy.


:Dendritic cells (DCs) exposed to tumor antigens followed by treatment with T(h)1-polarizing differentiation signals have paved the way for the development of DC-based cancer vaccines. Critical parameters for assessment of the optimal functional state of DCs and prediction of the vaccine potency of activated DCs have in the past been based on measurements of differentiation surface markers like HLA-DR, CD80, CD83, CD86, and CCR7 and the level of secreted cytokines like interleukin-12p70. However, the level of these markers does not provide a complete picture of the DC phenotype and may be insufficient for prediction of clinical outcome for DC-based therapy. We therefore looked for additional biomarkers by investigating the differential expression of microRNAs (miRNAs) in mature DCs relative to immature DCs. A microarray-based screening revealed that 12 miRNAs were differentially expressed in the two DC phenotypes. Of these, four miRNAs, hsa-miR-155, hsa-miR-146a, hsa-miR-125a-5p, and hsa-miR-29a, were validated by real-time polymerase chain reaction and northern blotting. The matured DCs from 12 individual donors were divided into two groups of highly and less differentiated DCs, respectively. A pronounced difference at the level of miRNA induction between these two groups was observed, suggesting that quantitative evaluation of selected miRNAs potentially can predict the immunogenicity of DC vaccines.


Hum Immunol


Human immunology


Holmstrøm K,Pedersen AW,Claesson MH,Zocca MB,Jensen SS




Has Abstract


2010-01-01 00:00:00














  • B cells in chronic graft-versus-host disease.

    abstract::Allogeneic hematopoietic stem cell transplantation (alloHCT) is the definitive therapy for numerous otherwise incurable hematologic malignancies and non-malignant diseases. The genetic disparity between donor and recipient both underpins therapeutic effects and confers donor immune system-mediated damage in the recipi...

    journal_title:Human immunology

    pub_type: 杂志文章,评审


    authors: McManigle W,Youssef A,Sarantopoulos S

    更新日期:2019-06-01 00:00:00

  • RFLP-defined HLA-DP polymorphism in four ethnic groups.

    abstract::Patterns of BglII, MspI, and TaqI DP alpha and DP beta hybridizing restriction fragment length polymorphisms are compared with primed lymphocyte typing--determined specificities in 28 10th International Histocompatibility Workshop core cell lines. Correlation of specific RFLP patterns with most recognized DP types is ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Easteal S,Voorhoeve AH,Zimmet P,Serjeantson SW

    更新日期:1989-07-01 00:00:00

  • Alleles and genotypes of polymorphisms of IL-18, TNF-α and IFN-γ are associated with a higher risk and severity of hepatocellular carcinoma (HCC) in Brazil.

    abstract::Hepatocellular carcinoma (HCC) is a primary malignant tumor of the liver. We evaluated the association of alleles and genotypes of polymorphisms of IL-18 (-607C/A and -137G/C), IFN-γ (+874T/A) and TNF-α (-238G/A and -308G/A) with the risk and severity of HCC. One-hundred-and-twelve patients with HCC and 202 healthy co...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Teixeira AC,Mendes CT Jr,Marano LA,Deghaide NH,Secaf M,Elias J Jr,Muglia V,Donadi EA,Martinelli AL

    更新日期:2013-08-01 00:00:00

  • Analysis of HLA-A/B recombinant families with new polymorphic markers.

    abstract::The MHC in humans is a much studied region of the genome, the genes of which display a high rate of polymorphism and a high rate of linkage disequilibrium. Four families in which intra-class-I recombination has occurred have been analyzed with six polymorphic markers between HLA-A and -B in order to determine the full...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Crouau-Roy B,Bouissou C,Sommer E,Pontarotti P,Thomsen M

    更新日期:1993-10-01 00:00:00

  • MUC1 is a novel costimulatory molecule of human T cells and functions in an AP-1-dependent manner.

    abstract::MUC1 mucin, primarily known as an epithelial antigen, has been demonstrated to be expressed on activated human T cells. In the present study, we first examined the expression of MUC1 on different subsets of T cells (naive, effector, effector/memory). MUC1 appears to be strongly upregulated on activated CD4(+) T cells ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Konowalchuk JD,Agrawal B

    更新日期:2012-05-01 00:00:00

  • Genetic polymorphisms of interleukin-6 modulate fibrosis progression in mild chronic hepatitis C.

    abstract::Genetic polymorphisms of interleukin-6 (IL-6) (-1363G>T, -597G>A, -572G>C, -174G>C, +2954G>C) may affect the outcomes of several diseases. This study was aimed to verify the role of these polymorphisms on the disease progression of patients with hepatitis C virus (HCV) infection and persistently normal transaminases (...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Falleti E,Fabris C,Vandelli C,Colletta C,Cussigh A,Smirne C,Fontanini E,Cmet S,Minisini R,Bitetto D,Toniutto P,Pirisi M

    更新日期:2010-10-01 00:00:00

  • Molecular complexity of HLA-DQw3: the TA10 determinant is located on a subset of DQw3 beta chains.

    abstract::In attempts to examine the relationships between serologic and structural polymorphisms of HLA-DQ molecules we have analyzed several monoclonal antibodies generated against polymorphic determinants on HLA-DQ molecules. One antibody, SFR20-DQw3, has a serologic reactivity like that of the previously characterized anti-...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Radka SF,Scott RG,Stewart SJ

    更新日期:1987-04-01 00:00:00

  • Bone marrow transplantation in Brazil.

    abstract::The first program of bone marrow transplantation in Latin America was started 5 years ago at the Federal University Hospital, Curitiba, Paraná. The results of 62 patients who underwent bone marrow transplantation are presented and discussed. ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Ferreira E,Dulley FL,Morsoletto F,Neto JZ,Pasquini R

    更新日期:1985-11-01 00:00:00

  • TAP2 gene polymorphism segregates with DR-DQ in DR/DP recombinant siblings.

    abstract::RING 11, a second transport-associated gene (TAP2), has been recently identified in the DR-DP interval of the human class II region. Two predominant alleles, TAP2A and TAP2B, differing by 17 amino acids at the C-terminus of the ATP-binding domain are present in the Caucasoid population at frequencies of 79% and 21%, r...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Eiermann TH,Fakler J,Goldmann SF,Böhm BO

    更新日期:1993-11-01 00:00:00

  • The influence of the hinge region length in binding of human IgG to human Fcgamma receptors.

    abstract::Interactions between human IgG with human FcgammaRI and FcgammaRIIa (R131) were studied to investigate the role of the hinge region of IgG3 and IgG1 in the binding of the antibodies to FcgammaR. It was found that a hinge deletion mutant of IgG3 (IgG3 m15) was reduced in its ability to bind to FcgammaRI and FcgammaRIIa...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Redpath S,Michaelsen TE,Sandlie I,Clark MR

    更新日期:1998-11-01 00:00:00

  • TAP1 and TAP2 gene polymorphisms and HLA-TAP haplotypes in Koreans based on 90 families.

    abstract::We have investigated the polymorphism of TAP genes and the distribution of human leukocyte antigen (HLA)-TAP haplotypes in 90 Korean families (180 parents and 233 children), previously typed for HLA class II alleles. TAP1 (codons 333 and 637) and TAP2 (codons 379, 565, 577, 651, 665, and 687) typings were carried out ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Whang DH,Park H,Roh EY,Park MH

    更新日期:2005-09-01 00:00:00

  • Structural aspects of HLA class I epitopes reacting with human monoclonal antibodies in Ig-binding, C1q-binding and lymphocytotoxicity assays.

    abstract::This study addresses the reactivity patterns of human cytotoxic HLA class I epitope-specific monoclonal antibodies in Ig-binding and complement component C1q-binding Luminex assays in comparison with complement-dependent lymphocytotoxicity data reported at the 13th International HLA Workshop. Some monoclonal antibodie...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Duquesnoy RJ,Marrari M,Jelenik L,Zeevi A,Claas FH,Mulder A

    更新日期:2013-10-01 00:00:00

  • Uniparental maternal disomy 6 in a renal transplant patient.

    abstract::HLA analysis of the family of a renal transplant patient revealed an extremely rare condition. On repeated typings the only demonstrable HLA antigens shown in the propositus were from the maternal haplotype, HLA-A11,-B46,-CW1,-DR14,-DQ1. No paternal antigens could be demonstrated either by serologic or by DNA-typing m...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: van den Berg-Loonen EM,Savelkoul P,van Hooff H,van Eede P,Riesewijk A,Geraedts J

    更新日期:1996-01-01 00:00:00

  • IL-4 polymorphisms, HRCT score and lung tissue markers in idiopathic pulmonary fibrosis.

    abstract:AIMS:We studied the influence of IL-4 gene polymorphisms on the IPF phenotype, i.e., extent of radiological changes (HRCT interstitial (IS) and alveolar (AS) score) and histopathological markers from lung biopsies. PATIENTS AND METHODS:46 IPF patients underwent genotyping, 43 of them had HRCT and 14 patients had a sur...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Vasakova M,Sterclova M,Matej R,Olejar T,Kolesar L,Skibova J,Striz I

    更新日期:2013-10-01 00:00:00

  • Variable clinical phenotypes of X-linked lymphoproliferative syndrome in China: Report of five cases with three novel mutations and review of the literature.

    abstract:BACKGROUND:X-linked lymphoproliferative disease (XLP) is a rare life-threatening syndrome. Rapid recognition and definitive diagnosis are critical to improve the prognosis and survival of patients with XLP. Nowadays, little is known about patients with XLP in China. METHODS:We report the characterization of five Chine...

    journal_title:Human immunology

    pub_type: 杂志文章,评审


    authors: Jin YY,Zhou W,Tian ZQ,Chen TX

    更新日期:2016-08-01 00:00:00

  • Cytomegalovirus (CMV)-specific cellular immune responses.

    abstract::A large percentage of healthy individuals (50-90%) is chronically infected with Cytomegalovirus (CMV). Over the past few years, several techniques were developed in order to monitor CMV-specific T-cell responses. In addition to the identification of antigen-specific T cells with peptide-loaded MHC complexes, most of t...

    journal_title:Human immunology

    pub_type: 杂志文章,评审


    authors: Harari A,Zimmerli SC,Pantaleo G

    更新日期:2004-05-01 00:00:00

  • Effect of an inhibitor of DNA methylation on T cells. II. 5-Azacytidine induces self-reactivity in antigen-specific T4+ cells.

    abstract::During T-cell maturation, thymocytes interact with thymic stromal major histocompatibility complex (MHC) determinants and thymic hormones, and proliferate, apparently in response to MHC gene products, in the absence of antigen. The maturing thymocytes also express a series of cell surface molecules, at one stage coexp...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Richardson B

    更新日期:1986-12-01 00:00:00

  • Impact of HLA-C and Bw epitopes disparity on liver transplantation outcome.

    abstract::The occurrence of graft rejection episodes after orthotopic liver transplantation (OLT) despite the use of immunosuppressive drugs designed to suppress T lymphocyte functions, indicates the involvement of other types of cells in this process. The activity of natural killer cells and their killer immunoglobulin-like re...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Bishara A,Brautbar C,Zamir G,Eid A,Safadi R

    更新日期:2005-11-01 00:00:00

  • Functional impact of A91V mutation of the PRF1 perforin gene.

    abstract::Perforin (PRF1) gene mutations have been associated with Familial Hemophagocytic Lymphohistiocytosis type 2 (FHL2). Substitution p.A91V (c.272C>T) in exon 2 was first described as a neutral polymorphism. Nonetheless, recent clinical evidence and functional assays, suggest a potential pathogenic role for p.A91V, especi...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Martínez-Pomar N,Lanio N,Romo N,Lopez-Botet M,Matamoros N

    更新日期:2013-01-01 00:00:00

  • Di-allelic alloantigenic systems on subsets of T cells.

    abstract::Sera obtained from multiparous women and some of other origin contain antibodies which react with antigens on T cell subsets. These antibodies recognize two distinct diallelic systems, one of which is mainly present on T gamma cells while the other is present on T mu cells. The sera that reacted with the T gamma cells...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: van Leeuwen A,Festenstein H,van Rood JJ

    更新日期:1982-04-01 00:00:00

  • Analysis of genes within the HLA region affecting susceptibility to ulcerative colitis.

    abstract::To clarify the molecular relationship between HLA loci and ulcerative colitis (UC) in Japanese patients, we performed HLA-DP genotyping by the PCR-RFLP method and studied tumor necrosis factor beta-chain genetic polymorphism by Southern hybridization, in addition to conventional serologic typing. Significant increase ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Sugimura K,Asakura H,Mizuki N,Inoue M,Hibi T,Yagita A,Tsuji K,Inoko H

    更新日期:1993-02-01 00:00:00

  • A murine monoclonal antibody (928) recognizing a new epitope formed with a combination of HLA-DPA1*0201 and DPB1*0301 gene products.

    abstract::A murine monoclonal antibody (mAb), 928, that recognizes a cell surface antigen (928 Ag) on a human Epstein-Barr virus-transformed fetal liver-derived lymphoid progenitor cell line (FL4.4) was generated. The 928 mAb reacted with only FL4.4; it did not react with any other 57 cell lines tested. Two color flowcytometry ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Kishi H,Okumura A,Tong JJ,Sugiyama E,Matsuno H,Minowada J,Kanai T,Nishimura Y,Muraguchi A

    更新日期:1997-08-01 00:00:00

  • FOXP3 expression and frequency of regulatory T cells in healed individuals from Leishmania major infection and the asymptomatic cases.

    abstract::Two groups of residents in an endemic area of Leishmania major infection in Iran with positive leishmanin skin tests who were either asymptomatic or had healed cutaneous leishmaniasis lesions were compared with respect to their T helper responses. The percentages of regulatory T cells (Treg; CD4(+)CD25(high) FoxP3(+))...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Bahrami F,Darabi H,Riazi-Rad F,Khaze V,Ajdary S,Alimohammadian MH

    更新日期:2014-10-01 00:00:00

  • HLA class II association with rheumatoid arthritis: facts and interpretations.

    abstract::We have reviewed the literature on the association of HLA class II with rheumatoid arthritis (RA). Strong linkage disequilibrium among DQB1, DQA1 and DRB1 alleles makes it difficult to evaluate the individual contribution of each locus. Nonetheless, there is a strong case for the role of DQB1*03 and *04 combined with ...

    journal_title:Human immunology

    pub_type: 杂志文章,评审


    authors: Zanelli E,Breedveld FC,de Vries RR

    更新日期:2000-12-01 00:00:00

  • Quantitation of soluble HLA class I heterodimers and beta 2-microglobulin in patients with active pulmonary tuberculosis.

    abstract::Beta 2m serum levels have been shown to be increased in patients with tuberculosis and HIV infection. We determined the stability of beta 2m and of sHLA-I dimers in serum, and then determined the levels of both molecules in 60 non-HIV-infected patients with active pulmonary tuberculosis and in 55 adult controls. The l...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Inostroza J,Muñoz P,Espinoza R,Millaqueo L,Diaz P,Leiva L,Sorensen R

    更新日期:1994-07-01 00:00:00

  • Streptococcus pyogenes type 5 M protein is an antigen, not a superantigen, for human T cells.

    abstract::M proteins are coiled-coil dimers expressed on group A streptococcal cell surfaces. They have an important role in host antistreptococcal immunity and in poststreptococcal autoimmune sequelae. Controversy has arisen regarding whether type 5 M proteins are superantigenic for human T cells. To investigate this, we have ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Degnan B,Taylor J,Hawkes C,O'Shea U,Smith J,Robinson JH,Kehoe MA,Boylston A,Goodacre JA

    更新日期:1997-04-01 00:00:00

  • Interaction of KIR genes and G1M immunoglobulin allotypes confer susceptibility to type 2 diabetes in Puerto Rican Americans.

    abstract::The susceptibility to type 2 diabetes (T2D) involves genetic factors. We studied the distribution of KIR and MHC class I ligands phenotype and genotype frequencies, as well as immunoglobulin KM and GM allotype frequencies in a group of patients (N = 95) with T2D and ethnically matched healthy controls (N = 74) with Pu...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Zuniga J,Romero V,Azocar J,Stern JN,Clavijo O,Almeciga I,Encinales L,Avendano A,Fridkis-Hareli M,Pandey JP,Yunis EJ

    更新日期:2006-11-01 00:00:00

  • The ABCs (DRDQDPs) of the prozone effect in single antigen bead HLA antibody testing: Lessons from our highly sensitized patients.

    abstract::Accurate identification of HLA antibodies using the single antigen bead (SAB) assay is critical for assessment of pre/post-transplant immunological risk and successful virtual crossmatching. Unfortunately, high titer HLA antibodies can be missed or underestimated in the SAB assay as a result of interference with the d...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Greenshields AL,Liwski RS

    更新日期:2019-07-01 00:00:00

  • Impaired circulating CD56dim NK cells are associated with decompensation of HBV-related cirrhosis.

    abstract::NK cells play an important role in immune regulation and defense of infection, but their characteristics in patients with decompensated cirrhosis and their relationship with liver function remain unclear. We studied the functional properties of NK cells (including CD56dim NK and CD56bright NK cells) in patients with H...

    journal_title:Human immunology

    pub_type: 临床试验,杂志文章


    authors: Jiang Y,Chen Y,Chen L,Yao W,Guan J,Liu X,Wei X,Lin X

    更新日期:2020-01-01 00:00:00

  • TCR expression and clonality analysis in peripheral blood and lymph nodes of HIV-infected patients.

    abstract::We compared the T cell receptor (TCR) V beta gene family repertoire in peripheral blood mononuclear cells (PBMC) and lymph node (LN) cells from 7 human immunodeficiency virus (HIV)-infected patients and 3 seronegative healthy controls. Virtually all the V beta family specificities were represented in patient PBMC and ...

    journal_title:Human immunology

    pub_type: 杂志文章


    authors: Mion M,Indraccolo S,Feroli F,Minuzzo S,Masiero S,Zamarchi R,Barelli A,Borri A,Chieco-Bianchi L,Amadori A

    更新日期:1997-10-01 00:00:00