Abstract：
:Mutations of Ki-ras and p53 genes in hepatocellular carcinomas (HCCs) induced by the choline deficient L-amino acid defined (CDAA) diet in rats were investigated by polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP) analysis followed by direct sequencing. Male Fischer 344 rats, 6 weeks old, were continuously given a CDAA diet for 70 weeks and then sacrificed. Macroscopically detectable nodules which were histologically confirmed to be well-differentiated HCCs were dissected free from the surrounding tissue and subjected to gene mutation analysis along with samples of non-tumor areas. Conformational change in the Ki-ras gene was detected in 1 out of 7 HCCs, involving a GGC to GTC transversion at codon 13. No p53 mutations were detected in 7 HCCs and also neither Ki-ras nor p53 mutations were found in non-tumor areas. The results suggest that neither Ki-ras nor p53 genes play an important role in hepatocarcinogenesis caused by long term expose to a CDAA diet in rats.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Tsujiuchi T,Kido A,Nakae D,Takahama M,Majima T,Kobitsu K,Okajima E,Tsutsumi M,Denda A,Konishi Ydoi
10.1016/s0304-3835(96)04405-9subject
Has Abstractpub_date
1996-11-12 00:00:00pages
137-41issue
1eissn
0304-3835issn
1872-7980pii
S0304383596044059journal_volume
108pub_type
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