Abstract:
:We have examined the effect of protease inhibitors on radiation-induced DNA amplification in vitro using an SV40-transformed Chinese hamster embryo cell line. DNA from cells irradiated with X-rays (10 Gy) or ultraviolet radiation (8 J/m2) resulted in an 8-fold or greater amplification of SV40 sequences compared with unirradiated cells. Addition of antipain or the soybean-derived Bowman-Birk protease inhibitor (BBI) to the culture medium, either 75 min after X-irradiation or within 15 min after ultraviolet irradiation, resulted in a reduction of amplification levels. BBI was more effective than antipain at an equal concentration. These results suggest that a protease may be involved in DNA amplification.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Flick MB,Kennedy ARdoi
10.1016/0304-3835(91)90083-tsubject
Has Abstractpub_date
1991-02-01 00:00:00pages
103-8issue
2eissn
0304-3835issn
1872-7980pii
0304-3835(91)90083-Tjournal_volume
56pub_type
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