Abstract:
:Gene methylation is an important molecular event in prostate carcinogenesis that may have diagnostic and prognostic significance. We evaluated the methylation status of eight genes implicated in prostate carcinogenesis. DNA was extracted from archived paraffin-embedded tumor blocks from 90 prostate cancer patients. Gene methylation status of eight genes (GSTP1, RASSF1A, RARbeta2, CD44, EDNRB, E-cadherin, Annexin-2, and Caveolin-1) was determined using real-time methylation-sensitive PCR techniques. Differences in gene methylation among race, tumor grade and disease stage were evaluated by chi-square test. Of the eight genes, GSTP1, RASSF1A, and RARbeta2 methylation was highly prevalent across tumors (>60% for all three genes) whereas CD44, E-cadherin and EDNRB methylation was less prevalent (33, 24 and 29%, respectively). Annexin-2 and Caveolin-1 were not methylated in any of the tumors examined. Methylation of RARbeta2, CD44 and E-cadherin was correlated with tumor grade but not stage. Interestingly, methylation of EDNRB, a gene involved in angiogenesis, was correlated with stage of disease but not tumor grade. With the possible exception of CD44, we did not observe differences in gene methylation between racial categories for the genes under study. In summary, our data suggest that evaluation of the methylation of a panel of genes may have diagnostic and prognostic utility in prostate cancer.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Woodson K,Hanson J,Tangrea Jdoi
10.1016/j.canlet.2003.11.027keywords:
subject
Has Abstractpub_date
2004-03-18 00:00:00pages
181-8issue
2eissn
0304-3835issn
1872-7980pii
S030438350300819Xjournal_volume
205pub_type
杂志文章相关文献
CANCER LETTERS文献大全abstract::Because lipoperoxides seem able to modulate tumor growth, we have examined the concomitant effects of dietary antioxidant (vitamin E) and of a high amount of polyunsaturated fatty acids (PUFA) in a model of chemically-induced mammary carcinogenesis. Two groups of rats received a high fat diet with or without added vit...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(97)04671-5
更新日期:1997-03-19 00:00:00
abstract::Metastatic colonization involves paracrine/juxtacrine interactions with the microenvironment inducing an adaptive response through transcriptional regulation. However, the identities of transcription factors (TFs) induced by the metastatic microenvironment in ovarian cancer (OC) and their mechanism of action is poorly...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2017.11.012
更新日期:2018-02-01 00:00:00
abstract::Formation of DNA-adducts by 3-BHA or its metabolites, i.e., tert-butyl-1,4-benzoquinone (TBQ) and 5-methoxy-3-tert-butyl-1,2-benzoquinone (3-TBOQ), as well as DNA-adduct formation by 4-nitroquinoline-N-oxide (4NQO), in rat forestomach were examined by an enzymatic 32P-postlabeling assay. Four DNA-adducts were clearly ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(89)90117-1
更新日期:1989-12-01 00:00:00
abstract::Background is presented to suggest that a great many biologic processes are chaotic. It is well known that chaotic processes can be accurately characterized by non-linear technologies. Evidence is presented that an artificial neural network, which is a known method for the application of non-linear statistics, is able...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(94)90090-6
更新日期:1994-03-15 00:00:00
abstract::We constructed the plasmid which can express human wild-type p53 cDNA and introduced it into the human osteosarcoma cell line SAOS-2 that lacks the chromosomal p53 gene. A cell clone stably expressing p53 protein was isolated and UV sensitivity and UV-induced mutation frequencies of the clone were examined. The UV sen...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(97)00406-0
更新日期:1998-01-16 00:00:00
abstract::Interferon-beta (IFN-β) is reported to augment anti-tumor effects by temozolomide in glioblastoma via down-regulation of MGMT. Promyelocytic leukemia (PML), a gene induced by IFN-β, is a tumor suppressor. Here, we report for the first time that in combination therapy, an IFN-β-induced increase in endogenous PML contri...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2012.04.013
更新日期:2012-10-28 00:00:00
abstract::As a cellular degradation mechanism, autophagy exerts crucial and complicated effects on HCC development. Liver non-parenchymal cells, including hepatic resident macrophage Kupffer cells, also play important roles in this process. However, most associated studies have focused on the influence of the autophagy level in...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2016.12.004
更新日期:2017-03-01 00:00:00
abstract::Viscum album L. (VAL) is a phytopreparation used in adjuvant cancer therapy with both immunostimulatory and DNA stabilizing properties at low drug concentrations and cytostatic/cytotoxic properties at higher concentrations. The present work examines the cytotoxic effects of VAL extracts produced from mistletoes grown ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(95)04038-2
更新日期:1996-01-19 00:00:00
abstract::A novel iron chelator, HDp44mT, has been reported to have potent anti-proliferative effects on cancer cells; however, the underlying mechanism of action is not well understood. In this study, we characterized the cytotoxic effect of HDp44mT in a chemo- and radio-resistant cell line (PC-3) of prostatic cancer origin. T...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2010.07.010
更新日期:2010-12-08 00:00:00
abstract::The implementation of novel therapeutic interventions has improved the survival rates of melanoma patients with metastatic disease. Nonetheless, only 33% of treated cases exhibit long term responses. Circulating tumor cell (CTC) measurements are currently of clinical value in breast, prostate and colorectal cancers. H...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2018.03.013
更新日期:2018-06-28 00:00:00
abstract::Fanconi anemia (FA) is a rare genome instability syndrome characterized by progressive bone marrow failure and predisposition to cancer, especially head and neck squamous cell carcinoma. Surgical resection is the standard of care for solid tumors, as patients with FA do not tolerate genotoxic chemotherapies or radiati...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2019.12.008
更新日期:2020-03-01 00:00:00
abstract::Though significant progress has been made in the availability of diagnostic techniques and treatment strategies, pancreatic cancer remains a disease of high mortality rates. Therefore, there is an urgent need for a better understanding of the molecular mechanisms that governs the oncogenesis and metastasis process of ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2016.12.014
更新日期:2017-03-01 00:00:00
abstract::In experimental trials using the N-nitroso-N-methylurea (NMU)-induced rat mammary tumor model, a significant decrease in tumor incidence (to 5%) was observed in rats treated with melatonin and 9-cis-retinoic acid (9 cRA) compared to controls (55%). Although 9cRA alone decreased tumor incidence to 26%, this response di...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(01)00548-1
更新日期:2001-07-26 00:00:00
abstract::To identify non-tumor biomarkers for prediction of tumor HPV status and prognosis of patients with squamous cell carcinoma of the oropharynx (SCCOP), we evaluated the association of single nucleotide polymorphisms (SNPs) in pre-miRNAs with HPV16 status and survival for SCCOP patients. We analyzed HPV16 status in tumor...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2012.11.048
更新日期:2013-04-28 00:00:00
abstract:BACKGROUND AND AIMS:Understanding the molecular pathogenesis of hepatocellular carcinoma (HCC) is essential to identify therapeutic targets. A hepatitis B virus (HBV) related double transgenic murine model was developed. METHODS:Liver specific expression of HBV X protein (HBx) and insulin receptor substrate 1 (IRS1) w...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.09.016
更新日期:2016-01-01 00:00:00
abstract::A 65K protein, known for promoting nucleocytoplasmic mRNA transport in a cell-free system, was previously found in fetal and tumor cells of the rat. The primary objective of this study was to show specificity of immunohistochemical staining for the 65K protein in the livers of rats subjected to a hepatocarcinogenesis ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(89)90120-1
更新日期:1989-12-01 00:00:00
abstract::To investigate if genomic instability exists in tumors with cytogenetically normal karyotypes, we analyzed four diploid cancer cell lines A204, CAL51, CH1 and SK-UT-1B. We detected subtle genomic changes in all four cell lines. More of these alterations were found in A204 and CH1 than in the microsatellite unstable li...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2008.03.003
更新日期:2008-08-18 00:00:00
abstract::Hypusine formation on the eukaryotic initiation factor 5A (eIF-5A) precursor is ubiquitously present in eukaryotic cells and archebacteria. In this reaction, deoxyhypusine synthase catalyzes the conversion of one unique lysine residue on eIF-5A to deoxyhypusine using spermidine as the substrate. Hydroxylation of the d...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(97)04741-1
更新日期:1997-05-19 00:00:00
abstract::Experimental data were provided to demonstrate the inhibitory effects of retinoids (retinal, retinoic acid, retinyl acetate and retinyl palmitate) and carotenoids (beta-carotene, lycopene and crocetin) on the growth and development of the C-6 glioma cells inoculated in rats. In the pretreatment experiments, most of th...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(89)90050-5
更新日期:1989-11-30 00:00:00
abstract::Circulating glucose-regulated protein 78 (GRP78) autoantibodies represent potential biomarker of epithelial ovarian cancer. However there is relatively limited identification of these antibodies in response to ovarian cancer. Here, we were interested in characterization of these antibodies and into their role in tumor...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2011.05.022
更新日期:2011-10-01 00:00:00
abstract::Cancer results from genetic alterations in intracellular signaling pathways, which normally orchestrate the execution of developmental programs and the organismic response to extrinsic factors. Mutations in upstream activators and components of the cytoplasmic (Ras-Raf MEK-ERK) cascade frequently occur in tumors. In v...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2004.08.003
更新日期:2005-01-10 00:00:00
abstract::Bone metastasis is an important cause of morbidity in advanced prostate cancer. Bisphosphonates are widely used for the treatment and prevention of osteoporosis, but recently have been observed to be effective in controlling prostate cancer metastasis. Since aminopeptidase N (AP-N) is known to be involved in the metas...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2005.06.006
更新日期:2006-06-18 00:00:00
abstract::Human cytolytic fusion proteins (hCFPs) offer a promising immunotherapeutic approach for the treatment of solid tumors, avoiding the immunogenicity and undesirable side-effects caused by immunotoxins derived from plants or bacteria. The well-characterized human serine protease granzyme B has already been used as a the...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2016.02.020
更新日期:2016-05-01 00:00:00
abstract::Most brain tumors are highly resistant to chemotherapy because many chemotherapeutic drugs poorly cross the blood-brain barrier, the blood-cerebrospinal-fluid barrier, and the plasma membrane of the tumor cells. This restricted drug delivery is largely due to the presence of integral plasma membrane proteins belonging...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2006.12.023
更新日期:2007-08-28 00:00:00
abstract::Tumor-associated mesenchymal stem cells (MSCs) play a critical role in the growth and metastasis of hepatocellular carcinoma (HCC). However, the mechanism underlying the crosstalk between MSCs and HCC cells is not completely understood. Here, HCC cells were treated with or without conditioned medium of MSCs (CM-MSC), ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2021.01.011
更新日期:2021-01-17 00:00:00
abstract::The immunomodulatory and antimetastatic/antitumor activity of thymosin alpha(1) (Talpha(1)) was evaluated in BALB/c-mice. Daily subcutaneous application (7 consecutive days, 0.01-10 microg of Talpha(1)/injection per mouse) upregulated the number of thymocytes and peripheral blood cells in tumor bearing mice. To check ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(00)00510-3
更新日期:2000-10-16 00:00:00
abstract::We induced tolerance to hexadecylphosphocholine (HePC) in the human epidermoid tumor cell line, KB. After 70 weeks of adaptation, the IC50 of HePC in the resistant cells KBr was 32-fold higher than in parental KB cells, and they were 30-fold more resistant to another ether lipid analogue, ET-18-OCH3. The KBr cells als...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(99)00146-9
更新日期:1999-08-03 00:00:00
abstract::Human cancer cells in vitro (NHIK 3025), derived from a carcinoma in situ are inactivated when exposed to visible light in the presence of hematoporphyrin. Irradiation at 4 degrees C results in much more efficient inactivation than irradiation at 37 degrees C. Furthermore, a given total light dose is more efficient wh...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(79)80090-7
更新日期:1979-05-01 00:00:00
abstract::We evaluated the ability of brain-derived neurotrophic factor (BDNF) to decrease the chemosensitivity of neuroblastoma cells to cisplatin. Two cell lines, one derived from SH-SY5Y (SY5Y-TB8) and the other from SK-N-AS (AS-TB8), transfected with a TrkB plasmid were generated, and used to assess the effects of activatio...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(02)00723-1
更新日期:2003-04-10 00:00:00
abstract::The p53 signaling pathway plays critical roles in determining the radio-sensitivity of normal tissues but is frequently inactivated in cancer. In various cancer cells, p53 has different effects on radio-sensitivity through complex mechanisms. Acetylation of p53 is an important means of post-translational modifications...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2015.04.015
更新日期:2015-07-28 00:00:00