Abstract:
:We constructed the plasmid which can express human wild-type p53 cDNA and introduced it into the human osteosarcoma cell line SAOS-2 that lacks the chromosomal p53 gene. A cell clone stably expressing p53 protein was isolated and UV sensitivity and UV-induced mutation frequencies of the clone were examined. The UV sensitivity of the clone was slightly higher and UV-induced hprt mutation frequencies of the clone were markedly lower than those of parental SAOS-2 cells. The capability to repair UV-induced DNA damage assessed by the amount of unscheduled DNA synthesis or DNA single strand breaks as well as cell cycle progression after UV irradiation were not different between the clone and SAOS-2 cells. These results indicate that wild-type p53 protein would be involved in the human DNA damage-processing pathway other than the genome-overall excision repair.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Yagi T,Mohri-Nakanishi K,Matsuda T,Yamagishi N,Miyakoshi J,Takebe Hdoi
10.1016/s0304-3835(97)00406-0subject
Has Abstractpub_date
1998-01-16 00:00:00pages
71-6issue
1eissn
0304-3835issn
1872-7980pii
S0304-3835(97)00406-0journal_volume
123pub_type
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