Abstract:
:Cell-free transcription-translation is an expanding field in synthetic biology as a rapid prototyping platform for blueprinting the design of synthetic biological devices. Exemplar efforts include translation of prototype designs into medical test kits for on-site identification of viruses (Zika and Ebola), while gene circuit cascades can be tested, debugged and re-designed within rapid turnover times. Coupled with mathematical modelling, this discipline lends itself towards the precision engineering of new synthetic life. The next stages of cell-free look set to unlock new microbial hosts that remain slow to engineer and unsuited to rapid iterative design cycles. It is hoped that the development of such systems will provide new tools to aid the transition from cell-free prototype designs to functioning synthetic genetic circuits and engineered natural product pathways in living cells.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Moore SJ,MacDonald JT,Freemont PSdoi
10.1042/BST20170011subject
Has Abstractpub_date
2017-06-15 00:00:00pages
785-791issue
3eissn
0300-5127issn
1470-8752pii
BST20170011journal_volume
45pub_type
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