Abstract:
:Nucleosomes are actively positioned along DNA by ATP-dependent, chromatin remodelling factors. A structural model for the ISW1a chromatin remodelling factor from Saccharomyces cerevisiae in complex with a dinucleosome substrate was constructed from the X-ray structures of ISW1a (ΔATPase) with and without DNA bound, two different cryo-EM (cryo-electron microscopy) structures of ISW1a (ΔATPase) bound to a nucleosome, and site-directed photo-cross-linking analyses in solution. The X-ray structure of ISW1a (ΔATPase) with DNA bound suggests that DNA sequence may be involved in nucleosome recognition and thereby specificity of promoter interaction. The model suggests how the highly ordered nucleosome arrays observed by mapping nucleosomes in genes and their promoter regions could be generated by a chromatin remodelling factor.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Richmond TJdoi
10.1042/BST20110748subject
Has Abstractpub_date
2012-04-01 00:00:00pages
347-50issue
2eissn
0300-5127issn
1470-8752pii
BST20110748journal_volume
40pub_type
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