Abstract:
:Ribonucleic acids are driving a multitude of biological processes where they act alone or in complex with proteins (ribonucleoproteins, RNP). To understand these processes both structural and mechanistic information about RNA is necessary. Due to their conformational plasticity RNA pose a challenge for mainstream structural biology methods. Solid-state NMR (ssNMR) spectroscopy is an emerging technique that can be applied to biomolecular complexes of any size in close-to-native conditions. This review outlines recent methodological developments in ssNMR for structural characterization of RNA and protein-RNA complexes and provides relevant examples.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Sreemantula AK,Marchanka Adoi
10.1042/BST20191080subject
Has Abstractpub_date
2020-06-30 00:00:00pages
1077-1087issue
3eissn
0300-5127issn
1470-8752pii
225413journal_volume
48pub_type
杂志文章abstract::Mutations activating the PI3K (phosphoinositide 3-kinase)/Akt signalling pathway and inactivating the TP53 tumour-suppressor gene are common mechanisms that cancer cells require to proliferate and escape pre-programmed cell death. In a well-described mechanism, Akt mediates negative control of p53 levels through enhan...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20140070
更新日期:2014-08-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0341141
更新日期:2006-12-01 00:00:00
abstract::Laccases are oxidase enzymes produced by 'white rot' fungi as part of a complex armoury of redox enzymes used to break down lignin--part of the carbon cycle of nature. Laccases alone or in combination with redox co-catalysts have been shown to oxidize xenobiotic compounds under conditions that can be described as 'gre...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20060304
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20150127
更新日期:2015-10-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20051438
更新日期:2005-12-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0310134
更新日期:2003-02-01 00:00:00
abstract::ESR1 is frequently silenced by CGI (CpG island) methylation, both in human colorectal tumours and, in an age-dependent manner, in healthy mucosa. It is not clear, however, whether methylation of individual cytosines occurs randomly within the epithelial genome, or preferentially within individual cells as an 'all-or-n...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0330709
更新日期:2005-08-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20170443
更新日期:2018-10-19 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20120073
更新日期:2012-06-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0381474
更新日期:2010-12-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0391387
更新日期:2011-10-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0390128
更新日期:2011-01-01 00:00:00
abstract::In the present paper, we describe multiple levels of cross-talk between the PI3K (phosphoinositide 3-kinase)/Akt and Ras/MAPK (mitogen-activated protein kinase) signalling pathways. Experimental data and computer simulations demonstrate that cross-talk is context-dependent and that both pathways can activate or inhibi...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20110609
更新日期:2012-02-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0381527
更新日期:2010-12-01 00:00:00
abstract::The yeast Sir2 (silent information regulator-2) protein functions as an NAD(+)-dependent histone deacetylase to silence gene expression from the mating-type locus, tolomeres and rDNA and also promotes longevity and genome stability in response to calorie restriction. Homologues of yeast Sir2 have been identified in th...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0320904
更新日期:2004-12-01 00:00:00
abstract::Microvilli are found on the apical surface of epithelial cells. Recent studies on the microvillar proteins ezrin and EBP50 (ezrin/radixin/moesin-binding phosphoprotein of 50 kDa) have revealed both the dynamics and the regulation of microvillar components, and how a dynamic ezrin phosphocycle is necessary to confine m...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST20130263
更新日期:2014-02-01 00:00:00
abstract::GRIF-1 [GABA(A) (gamma-aminobutyric acid(A)) receptor interacting factor-1] is a member of a coiled-coil family of proteins thought to function as adaptors in the anterograde trafficking of organelles utilizing the kinesin-1 motor proteins to synapses. To study in more detail the molecular interaction between GRIF-1 a...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0340048
更新日期:2006-02-01 00:00:00
abstract::Mutations in the X-linked MECP2 gene are the primary cause of the severe autism spectrum disorder RTT (Rett syndrome). Deletion of Mecp2 in mice recapitulates many of the overt neurological features seen in humans, and the delayed onset of symptoms is accompanied by deficits in neuronal morphology and synaptic physiol...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0380498
更新日期:2010-04-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0360189
更新日期:2008-04-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0390563
更新日期:2011-04-01 00:00:00
abstract::PTB (polypyrimidine tract-binding protein) is an abundant and widely expressed RNA-binding protein with four RRM (RNA recognition motif) domains. PTB is involved in numerous post-transcriptional steps in gene expression in both the nucleus and cytoplasm, but has been best characterized as a regulatory repressor of som...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20120044
更新日期:2012-08-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0300487
更新日期:2002-08-01 00:00:00
abstract::To investigate the roles of the PTEN (phosphatase and tensin homologue deleted from chromosome 10)/PI3K (phosphoinositide 3-kinase) signalling pathway in vivo, we have generated a series of mutant mice with null or tissue-specific gene-targeted deletions of Pten. Here we present our investigations of Pten function in ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0320362
更新日期:2004-04-01 00:00:00
abstract::Many Zn(II)2Cys6 transcriptional regulators exhibit changes in phosphorylation that are coincident with their roles in transcriptional activation. It is, however, unclear whether these changes occur as a cause of, or as a result of, transcriptional activation. In this paper, we explore the relationship between these t...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0340794
更新日期:2006-11-01 00:00:00
abstract::SUMO (small ubiquitin-related modifier) modification is emerging as an important post-translational control in transcription. In general, SUMO modification is associated with transcriptional repression. Although many SUMO-modified transcription factors and co-activators have been identified, little is known about the ...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST0351397
更新日期:2007-12-01 00:00:00
abstract::Our first experience of mannan-binding lectin (MBL)-replacement therapy was with a patient experiencing recurrent erythema multiforme associated with reactivation of herpes simplex virus; his erythematous eruptions could be controlled with infusions of fresh frozen plasma containing MBL, but not with plasma lacking MB...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/bst0310768
更新日期:2003-08-01 00:00:00
abstract::The majority of mRNAs in eukaryotic cells are translated via a method that is dependent upon the recognition of, and binding to, the methylguanosine cap at the 5' end of the mRNA, by a set of protein factors termed eIFs (eukaryotic initiation factors). However, many of the eIFs involved in this process are modified an...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0381581
更新日期:2010-12-01 00:00:00
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journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/bst0311062
更新日期:2003-10-01 00:00:00
abstract::Coagulation proteases are involved in a highly orchestrated proteolytic cascade which is essential for haemostasis and blood clotting. In particular, the initiator of the coagulation cascade, Factor VIIa, binds to its cofactor, tissue factor, and its substrate, Factor X, via exosite interactions to form a ternary cata...
journal_title:Biochemical Society transactions
pub_type: 杂志文章
doi:10.1042/BST0350555
更新日期:2007-06-01 00:00:00
abstract::Mutations in the LMNA gene, encoding the nuclear intermediate filaments the A-type lamins, result in a wide variety of diseases known as laminopathies. Some of them, such as familial partial lipodystrophy of Dunnigan and metabolic laminopathies, are characterized by lipodystrophic syndromes with altered fat distributi...
journal_title:Biochemical Society transactions
pub_type: 杂志文章,评审
doi:10.1042/BST20110675
更新日期:2011-12-01 00:00:00