Abstract:
:The binding sites on human IgG1 for human Fc gamma receptor (Fc gamma R) I, Fc gamma RIIa, Fc gamma RIIb, Fc gamma RIIIa and neonatal FcR have been mapped. A common set of IgG1 residues is involved in binding to all Fc gamma Rs, while Fc gamma RII and Fc gamma RIII utilize distinct sites outside this common set. In addition to residues which abrogated binding to the Fc gamma R, several positions were found which improved binding only to specific Fc gamma Rs or simultaneously improved binding to one type of Fc gamma R and reduced binding to another type. Selected IgG1 variants with improved binding to Fc gamma RIIIa were then tested in an in vitro antibody-dependent cellular cytotoxicity (ADCC) assay and showed an enhancement in ADCC when either peripheral blood mononuclear cells or natural killer cells were used.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Presta LG,Shields RL,Namenuk AK,Hong K,Meng YGdoi
10.1042/bst0300487keywords:
subject
Has Abstractpub_date
2002-08-01 00:00:00pages
487-90issue
4eissn
0300-5127issn
1470-8752journal_volume
30pub_type
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