CRISPR base editing applications for identifying cancer-driving mutations.

Abstract:

:CRISPR base editing technology is a promising genome editing tool as (i) it does not require a DNA template to introduce mutations and (ii) it avoids creating DNA double-strand breaks, which can lead to unintended chromosomal alterations or elicit an unwanted DNA damage response. Given many cancers originate from point mutations in cancer-driving genes, the application of base editing for either modelling tumour development, therapeutic editing, or functional screening is of great promise. In this review, we summarise current DNA base editing technologies and will discuss recent advancements and existing hurdles for its usage in cancer research.

journal_name

Biochem Soc Trans

authors

Pal M,Herold MJ

doi

10.1042/BST20200550

subject

Has Abstract

pub_date

2021-01-15 00:00:00

eissn

0300-5127

issn

1470-8752

pii

227608

pub_type

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