Structure of artificial and natural VE-cadherin-based adherens junctions.

Abstract:

:In vascular endothelium, adherens junctions between endothelial cells are composed of VE-cadherin (vascular endothelial cadherin), an adhesive receptor that is crucial for the proper assembly of vascular structures and the maintenance of vascular integrity. As a classical cadherin, VE-cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. Although, from structural crystallographic data, a dimeric structure arranged in a trans orientation has emerged as a potential mechanism of cell-cell adhesion, the cadherin organization within adherens junctions remains controversial. Concerning VE-cadherin, its extracellular part possesses the capacity to self-associate in solution as hexamers consisting of three antiparallel cadherin dimers. VE-cadherin-based adherens junctions were reconstituted in vitro by assembly of a VE-cadherin EC (extracellular repeat) 1-EC4 hexamer at the surfaces of liposomes. The artificial adherens junctions revealed by cryoelectron microscopy appear as a two-dimensional self-assembly of hexameric structures. This cadherin organization is reminiscent of that found in native desmosomal junctions. Further structural studies performed on native VE-cadherin junctions would provide a better understanding of the cadherin organization within adherens junctions. Homophilic interactions between cadherins are strengthened intracellularly by connection to the actin cytoskeleton. Recently, we have discovered that annexin 2, an actin-binding protein connects the VE-cadherin-catenin complex to the actin cytoskeleton. This novel link is labile and promotes the endothelial cell switch from a quiescent to an angiogenic state.

journal_name

Biochem Soc Trans

authors

Taveau JC,Dubois M,Le Bihan O,Trépout S,Almagro S,Hewat E,Durmort C,Heyraud S,Gulino-Debrac D,Lambert O

doi

10.1042/BST0360189

subject

Has Abstract

pub_date

2008-04-01 00:00:00

pages

189-93

issue

Pt 2

eissn

0300-5127

issn

1470-8752

pii

BST0360189

journal_volume

36

pub_type

杂志文章,评审
  • Pantothenate biosynthesis in higher plants.

    abstract::Pantothenate (vitamin B5) is a water-soluble vitamin essential for the synthesis of CoA and ACP (acyl-carrier protein, cofactors in energy yielding reactions including carbohydrate metabolism and fatty acid synthesis. Pantothenate is synthesized de novo by plants and micro-organisms; however, animals obtain the vitami...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0330743

    authors: Coxon KM,Chakauya E,Ottenhof HH,Whitney HM,Blundell TL,Abell C,Smith AG

    更新日期:2005-08-01 00:00:00

  • Cell communication across gap junctions: a historical perspective and current developments.

    abstract::Collaborative communication lies at the centre of multicellular life. Gap junctions (GJs) are surface membrane structures that allow direct communication between cells. They were discovered in the 1960s following the convergence of the detection of low-resistance electrical interactions between cells and anatomical st...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST20150056

    authors: Evans WH

    更新日期:2015-06-01 00:00:00

  • Biogenesis of the signal recognition particle.

    abstract::Assembly of ribonucleoprotein complexes is a facilitated quality-controlled process that typically includes modification to the RNA component from precursor to mature form. The SRP (signal recognition particle) is a cytosolic ribonucleoprotein that catalyses protein targeting to the endoplasmic reticulum. Assembly of ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0381093

    authors: Leung E,Brown JD

    更新日期:2010-08-01 00:00:00

  • RNA pseudoknots and the regulation of protein synthesis.

    abstract::RNA pseudoknots are structural elements found in almost all classes of RNA. Pseudoknots form when a single-stranded region in the loop of a hairpin base-pairs with a stretch of complementary nucleotides elsewhere in the RNA chain. This simple folding strategy is capable of generating a large number of stable three-dim...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0360684

    authors: Brierley I,Gilbert RJ,Pennell S

    更新日期:2008-08-01 00:00:00

  • Quinone-reactive proteins devoid of haem b form widespread membrane-bound electron transport modules in bacterial respiration.

    abstract::Many quinone-reactive enzyme complexes that are part of membrane-integral eukaryotic or prokaryotic respiratory electron transport chains contain one or more haem b molecules embedded in the membrane. In recent years, various novel proteins have emerged that are devoid of haem b but are thought to fulfil a similar fun...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0361011

    authors: Simon J,Kern M

    更新日期:2008-10-01 00:00:00

  • Tetraspanin CD9 in cell migration.

    abstract::Tetraspanin CD9 is associated with integrin adhesion receptors and it was reported that CD9 regulates integrin-dependent cell migration and invasion. Pro- and anti-migratory effects of CD9 have been linked to adhesion-dependent signalling pathways, including phosphorylation of FAK (focal adhesion kinase) and activatio...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0390563

    authors: Powner D,Kopp PM,Monkley SJ,Critchley DR,Berditchevski F

    更新日期:2011-04-01 00:00:00

  • Cytochrome P450 (CYP) mutants and substrate-specificity alterations: segment-directed mutagenesis applied to human CYP1A1.

    abstract::Cytochrome P450 (CYP) enzymes represent a large superfamily that displays extraordinarily diverse substrate specificities. After a concise review about CYPs of the CYP1A subfamily, which plays a crucial role in procarcinogen activation, this paper presents segment-directed mutagenesis. This approach generates a librar...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/0300-5127:0290128

    authors: Urban P,Jobert AS,Lainé R,Pompon D

    更新日期:2001-05-01 00:00:00

  • Acetylation of cyclin A: a new cell cycle regulatory mechanism.

    abstract::Cyclin A must be degraded at prometaphase in order to allow mitosis progression. Nevertheless, the signals that trigger cyclin A degradation at mitosis have been largely elusive. In the present paper, we review the status of cyclin A degradation in the light of recent evidence indicating that acetylation plays a role ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0380083

    authors: Mateo F,Vidal-Laliena M,Pujol MJ,Bachs O

    更新日期:2010-02-01 00:00:00

  • The Golgi ribbon: mechanisms of maintenance and disassembly during the cell cycle.

    abstract::The Golgi complex (GC) has an essential role in the processing and sorting of proteins and lipids. The GC of mammalian cells is composed of stacks of cisternae connected by membranous tubules to create a continuous network, the Golgi ribbon, whose maintenance requires several core and accessory proteins. Despite this ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST20190646

    authors: Ayala I,Mascanzoni F,Colanzi A

    更新日期:2020-02-28 00:00:00

  • Physiological significance of L-amino acid sensing by extracellular Ca(2+)-sensing receptors.

    abstract::The calcium-sensing receptor is a multimodal, multimetabolic sensor that mediates the feedback-dependent control of whole body calcium metabolism. Remarkably, in addition to its role in Ca(2+)(o) (extracellular Ca(2+)) sensing, the CaR (Ca(2+)-sensing receptor) also responds to L-amino acids. L-amino acids appear to a...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0351195

    authors: Conigrave AD,Mun HC,Brennan SC

    更新日期:2007-11-01 00:00:00

  • The nucleolus-like and precursor bodies of mammalian oocytes and embryos and their possible role in post-fertilization centromere remodelling.

    abstract::In nearly all somatic cells, the ribosome biosynthesis is a key activity. The same is true also for mammalian oocytes and early embryos. This activity is intimately linked to the most prominent nuclear organelles - the nucleoli. Interestingly, during a short period around fertilization, the nucleoli in oocytes and emb...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/BST20190847

    authors: Fulka H,Rychtarova J,Loi P

    更新日期:2020-04-29 00:00:00

  • Coupling the dynamics of two actin networks--new views on the mechanics of cell protrusion.

    abstract::We study how mechanical forces integrate spatially and temporally with regulatory signals at the leading edge of migrating cells. To probe the dynamics of this system, we developed quantitative fluorescent speckle microscopy, which maps out actin cytoskeleton transport, assembly and disassembly with high spatial resol...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/BST20051250

    authors: Danuser G

    更新日期:2005-12-01 00:00:00

  • Mitotic motors and chromosome segregation: the mechanism of anaphase B.

    abstract::Anaphase B spindle elongation plays an important role in chromosome segregation. In the present paper, we discuss our model for anaphase B in Drosophila syncytial embryos, in which spindle elongation depends on an ip (interpolar) MT (microtubule) sliding filament mechanism generated by homotetrameric kinesin-5 motors ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0391149

    authors: Brust-Mascher I,Scholey JM

    更新日期:2011-10-01 00:00:00

  • Nutritional status, metabolic responses to exercise and implications for performance.

    abstract::Diet can significantly influence exercise performance. Diet alone will not ensure successful performance in sport, but it plays a permissive role in allowing sustained intensive training with limited risk of illness and injury. Diet may also promote adaptations to training by stimulating synthesis and breakdown of the...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/bst0311267

    authors: Maughan RJ

    更新日期:2003-12-01 00:00:00

  • Targeting IRFs by ubiquitination: regulating antiviral responses.

    abstract::The IRF [IFN (interferon) regulatory factor] family of transcription factors control many cellular processes, including induction of key antiviral cytokines, type I IFNs, following viral infection. Recent studies have revealed several endogenous and viral proteins involved in ubiquitin-mediated regulation of IRF activ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0360453

    authors: Higgs R,Jefferies CA

    更新日期:2008-06-01 00:00:00

  • Exosomes as a novel way of interneuronal communication.

    abstract::Exosomes are small extracellular vesicles which stem from endosomes fusing with the plasma membrane; they contain lipids, proteins and RNAs that are able to modify receiving cells. Functioning of the brain relies on synapses, and certain patterns of synaptic activity can change the strength of responses at sparse grou...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST20120266

    authors: Chivet M,Javalet C,Hemming F,Pernet-Gallay K,Laulagnier K,Fraboulet S,Sadoul R

    更新日期:2013-02-01 00:00:00

  • Understanding non-coding DNA regions in yeast.

    abstract::Non-coding transcripts play an important role in gene expression regulation in all species, including budding and fission yeast. Such regulatory transcripts include intergenic ncRNA (non-coding RNA), 5' and 3' UTRs, introns and antisense transcripts. In the present review, we discuss advantages and limitations of rece...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST20130144

    authors: Schlackow M,Gullerova M

    更新日期:2013-12-01 00:00:00

  • Btk-dependent regulation of phosphoinositide synthesis.

    abstract::Activation of the BCR (B cell antigen receptor) stimulates the production of both PtdIns(3,4,5) P3 and Ins(1,4,5) P3. PtdIns(3,4,5) P3 and Ins(1,4,5) P3 are generated from a common substrate, PtdIns(4,5) P2. In some systems, continuous PtdIns(4,5) P2 synthesis is necessary for maximal Ins(1,4,5) P3 production, but whe...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/bst0320326

    authors: Carpenter CL

    更新日期:2004-04-01 00:00:00

  • Touché! STARD3 and STARD3NL tether the ER to endosomes.

    abstract::Membrane contact sites (MCSs) are subcellular regions where the membranes of distinct organelles come into close apposition. These specialized areas of the cell, which are involved in inter-organelle metabolite exchange, are scaffolded by specific complexes. STARD3 [StAR (steroidogenic acute regulatory protein)-relate...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST20150269

    authors: Wilhelm LP,Tomasetto C,Alpy F

    更新日期:2016-04-15 00:00:00

  • Red light, green light: probing single molecules using alternating-laser excitation.

    abstract::Single-molecule fluorescence methods, particularly single-molecule FRET (fluorescence resonance energy transfer), have provided novel insights into the structure, interactions and dynamics of biological systems. ALEX (alternating-laser excitation) spectroscopy is a new method that extends single-molecule FRET by provi...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/BST0360738

    authors: Santoso Y,Hwang LC,Le Reste L,Kapanidis AN

    更新日期:2008-08-01 00:00:00

  • The role of TDP-43 in the pathogenesis of ALS and FTLD.

    abstract::TDP-43 (TAR DNA-binding protein 43) is an hnRNP (heterogeneous nuclear ribonucleoprotein) protein whose role in cellular processes has come to the forefront of neurodegeneration research after the observation that it is the main component of brain inclusions in ALS (amyotrophic lateral sclerosis) and FTLD (frontotempo...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST20130186

    authors: Baralle M,Buratti E,Baralle FE

    更新日期:2013-12-01 00:00:00

  • Developing chemokine mutants with improved proteoglycan affinity and knocked-out GPCR activity as anti-inflammatory recombinant drugs.

    abstract::The interaction of chemokines and GAGs (glycosaminoglycans) on endothelial surfaces is a crucial step for establishing a chemotactic gradient which leads to the functional presentation of chemokines to their GPCRs (G-protein-coupled receptors) and thus to activation of approaching leucocytes. Based on molecular modell...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0340435

    authors: Potzinger H,Geretti E,Brandner B,Wabitsch V,Piccinini AM,Rek A,Kungl AJ

    更新日期:2006-06-01 00:00:00

  • A probabilistic model for the extraction of expression levels from oligonucleotide arrays.

    abstract::In this work we present a probabilistic model to estimate summaries of Affymetrix GeneChip probe level data. Comparisons with two different models were made both on a publicly available dataset and on a study performed in our laboratory, showing that our model performs better for consistency of fold change. ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/bst0311510

    authors: Milo M,Fazeli A,Niranjan M,Lawrence ND

    更新日期:2003-12-01 00:00:00

  • Molecular mechanism of force generation by dynein, a molecular motor belonging to the AAA+ family.

    abstract::Dynein is an AAA+ (ATPase associated with various cellular activities)-type motor complex that utilizes ATP hydrolysis to actively drive microtubule sliding. The dynein heavy chain (molecular mass >500 kDa) contains six tandemly linked AAA+ modules and exhibits full motor activities. Detailed molecular dissection of t...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0360131

    authors: Numata N,Kon T,Shima T,Imamula K,Mogami T,Ohkura R,Sutoh K,Sutoh K

    更新日期:2008-02-01 00:00:00

  • The quest to achieve the detailed structural and functional characterization of CymA.

    abstract::Shewanella oneidensis MR-1 is a sediment organism capable of dissimilatory reduction of insoluble metal compounds such as those of Fe(II) and Mn(IV). This bacterium has been used as a model organism for potential applications in bioremediation of contaminated environments and in the production of energy in microbial f...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST20120114

    authors: Louro RO,Paquete CM

    更新日期:2012-12-01 00:00:00

  • An integrated transcriptional switch at the β-selection checkpoint determines T cell survival, development and leukaemogenesis.

    abstract::In T cell development, a pivotal decision-making stage, termed β-selection, integrates a TCRβ checkpoint to coordinate survival, proliferation and differentiation to an αβ T cell. Here, we review how transcriptional regulation coordinates fate determination in early T cell development to enable β-selection. Errors in ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST20180414

    authors: Chann AS,Russell SM

    更新日期:2019-08-30 00:00:00

  • Circulating levels of the chemokines JE and KC in female C3H apolipoprotein-E-deficient and C57BL apolipoprotein-E-deficient mice as potential markers of atherosclerosis development.

    abstract::We have investigated serum chemokines for their suitability as markers of atherosclerosis development in apoE (apolipoprotein E)-deficient ((-/-)) mice. Female C3H apoE(-/-) and C57BL apoE(-/-) mice were fed on either diet W (Western diet; 6 weeks) or normal rodent diet (12 weeks). Serum lipids (0, 6 and 12 weeks) and...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/bst0320128

    authors: Parkin SL,Pritchett JP,Grimsditch DC,Bruckdorfer KR,Sahota PK,Lloyd A,Overend P,Benson GM

    更新日期:2004-02-01 00:00:00

  • Analysis of LRRK2 accessory repeat domains: prediction of repeat length, number and sites of Parkinson's disease mutations.

    abstract::Various investigators have identified the major domain organization of LRRK2 (leucine-rich repeat kinase 2), which includes a GTPase ROC (Ras of complex proteins) domain followed by a COR (C-terminal of ROC) domain and a protein kinase domain. In addition, there are four domains composed of structural repeat motifs li...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST20120088

    authors: Mills RD,Mulhern TD,Cheng HC,Culvenor JG

    更新日期:2012-10-01 00:00:00

  • Dynamic regulation of the voltage-gated Kv2.1 potassium channel by multisite phosphorylation.

    abstract::Voltage-gated K(+) channels are key regulators of neuronal excitability. The Kv2.1 voltage-gated K(+) channel is the major delayed rectifier K(+) channel expressed in most central neurons, where it exists as a highly phosphorylated protein. Kv2.1 plays a critical role in homoeostatic regulation of intrinsic neuronal e...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审

    doi:10.1042/BST0351064

    authors: Mohapatra DP,Park KS,Trimmer JS

    更新日期:2007-11-01 00:00:00

  • Molecular aspects of beta-ketoacyl synthase (KAS) catalysis.

    abstract::Crystal structure data for Escherichia coli beta-ketoacyl synthase (KAS) I with C(10) and C(12) fatty acid substrates bound in conjunction with results from mutagenizing residues in the active site leads to a model for catalysis. Differences from and similarities to the other Claisen enzymes carrying out decarboxylati...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章

    doi:10.1042/0300-5127:0280601

    authors: von Wettstein-Knowles P,Olsen J,Arnvig Mcguire K,Larsen S

    更新日期:2000-12-01 00:00:00