Abstract:
:We investigated the potential of Nicotiana benthamiana to express the E7 protein of human papillomavirus 8 (HPV-8), a paradigm genotype among cutaneous HPVs. The protein, modified in its putative pRb-binding domain (E7(QGD)), was transiently expressed in leaves following infiltration with agrobacteria carrying either a binary vector combined with silencing suppressor constructs or replicating tobacco mosaic virus (TMV)-based vectors with different targeting signals. HPV-8 E7(QGD) yields ranged from 250 ng to 4.6 mg per gram of fresh leaf tissue. The highest yields were obtained with TMV-based vectors targeting the antigen to the apoplast. HPV8-CER (H2(q)) mice transformed with the complete early region of HPV-8 showed a delay in the onset of skin papillomatous lesions and produced E7-specific immunoglobulins G when inoculated subcutaneously with leaf extracts expressing E7(QGD). Furthermore, we demonstrated that the plant-made HPV-8 E7(QGD) induced a specific cytotoxic response in C57BL/6 (H2(b)) mice.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Noris E,Poli A,Cojoca R,Rittà M,Cavallo F,Vaglio S,Matic S,Landolfo Sdoi
10.1007/s00705-010-0893-8subject
Has Abstractpub_date
2011-04-01 00:00:00pages
587-95issue
4eissn
0304-8608issn
1432-8798journal_volume
156pub_type
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journal_title:Archives of virology
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