Analysis of bovine herpes virus-type 1 isolates by restriction endonuclease fingerprinting.

Abstract:

:In an effort to determine whether distinct types of bovine herpesvirus 1 are responsible for causing specific syndromes, the polypeptides and DNA of 93 BHV 1 isolates from the province of Alberta as well as vaccine strains and numerous other Canadian, U.S. and European isolates were analyzed by PAGE and restriction endonuclease fingerprinting, respectively. The polypeptide patterns showed only slight variations: only six isolates contained polypeptides that varied from the norm in their molecular weight, or were absent. Although on the basis of endonuclease patterns the isolates could be categorized into three "strains" and nine "sub-strains", we were unable to associate any of the strains with specific clinical signs. This suggests that the type of disease caused may be determined more by the route of infection and animal management practices than by the inherent properties of certain types of BHV 1. Most of the Albertan isolates were of one BHV 1 type and sub-type.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Misra V,Babiuk LA,Darcel CL

doi

10.1007/BF01311201

subject

Has Abstract

pub_date

1983-01-01 00:00:00

pages

341-54

issue

4

eissn

0304-8608

issn

1432-8798

journal_volume

76

pub_type

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