Abstract：
:We examined the pattern of the N gene-mediated systemic hypersensitive response (HR), which was induced by tobacco mosaic virus upon temperature shift, and analyzed the distribution of the coat protein and the virus-encoded 126 kDa replicase protein (126 K protein) by immunoblot analysis. In the middle- and lower-positioned leaves, HR occurred in the advancing edge of the infected area, where we detected both the coat protein and the 126 K protein. In the areas between the main vein and the advancing edge of these leaves, we observed no HR and did not detect 126 K protein, though virus was present in these areas. In the upper-positioned mosaic leaves, patterns of the HR were different depending on the leaf age. In these mosaic leaves, the mechanism preventing the virus from invading dark green tissue seemed to be broken down in 8-14 days old leaves, and HR was observed only in the tissue just invaded by the virus, where we detected the 126 K protein. Those results suggested that the viral 126 K protein was present when the viral replication was taking place, and easily degraded when the amount of the virus was saturated in the cells.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Someya N,Niinuma K,Kimura M,Yamaguchi I,Hamamoto Hdoi
10.1007/s00705-004-0373-0keywords:
subject
Has Abstractpub_date
2004-11-01 00:00:00pages
2105-13issue
11eissn
0304-8608issn
1432-8798journal_volume
149pub_type
杂志文章abstract：:Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). We used microchip electrophoresis in combination with automatic image analysis to develop a novel high-throughput PCR-RFLP to type the gene sequences that encode BLV Tax 233. This method revealed that 233L-Tax is more prevalent than ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04842-w
更新日期：2020-12-01 00:00:00
abstract：:Intravenous polyoma virus inoculation into pregnant Wistar rats resulted in transplacental infection of the foetus, causing tumours and hydronephroses. Cyclosporin A reduced these effects significantly. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313783
更新日期：1993-01-01 00:00:00
abstract：:Rabbit anti-idiotypic antibodies (anti-ids) were generated against three bank vole and one human monoclonal antibody (MAb) specific for the two envelope glycoproteins of Puumala virus (G1 and G2). The anti-ids were purified by sequential immunoaffinity chromatography. Each anti-id inhibited the antigen binding of its ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309537
更新日期：1993-01-01 00:00:00
abstract：:Novel protoparvoviruses genetically related to human and non-human primate bufaviruses (BuVs) have been detected recently in respiratory and enteric specimens collected from dogs and cats. In this study, by molecular screening of archival collections of faecal samples from wolves and foxes, we detected BuVs with a rat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04304-y
更新日期：2019-09-01 00:00:00
abstract：:In this study, we obtained the whole genomes of three porcine bocaparvovirus (PBoV) strains (GD6, GD10, and GD23) by polymerase chain reaction. Sequence analysis showed that all three field strains belonged to PBoV group 3 (G3). The phylogenetic trees based on NS1, NP1, and VP1 differed to the extent that these PBoVs ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3606-8
更新日期：2018-03-01 00:00:00
abstract：:An electron microsopic study of cell cultures and bovine foetal tracheal organ cultures infected with infectious bovine rhinotracheitis (IBR) virus showed the following. a) The difference in the site at which the outer envelope is acquired in the infected cell is responsible for some morphological differences between ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317842
更新日期：1976-01-01 00:00:00
abstract：:Swine testicle cell lines were established by transformation of primary swine testicle (PST) cells with an SV40 plasmid (pSV3-neo), which contains genes conferring resistance to neomycin and expressing SV40 large T antigen. Plasmid DNA was transfected into PST cells using a lipofection system. Two related plasmids, pS...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310532
更新日期：1990-01-01 00:00:00
abstract：:A papovavirus was isolated from the urine of a 24 year old female who underwent bone marrow transplantation after a relapse of acute myeloid leukemia. The virus (JL) resembles BK virus in its antigenic and growth properties, but has a different restriction endonuclease pattern after digestion with the restriction endo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315635
更新日期：1978-01-01 00:00:00
abstract：:This study aimed at investigating the effect of progesterone on interferon signaling pathways in peripheral blood mononuclear cells (PBMCs) of patients infected with hepatitis C virus (HCV). PBMCs were isolated from peripheral blood of 38 treatment-naïve HCV-infected patients, pooled, and stimulated with progesterone ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1673-z
更新日期：2013-08-01 00:00:00
abstract：:The small intestine of piglets orally infected with rotavirus was examined by electron microscopy 18, 24, 48 and 60 hours after infection. At 18 and 24 hours after infection columnar epithelial cells covered the villi. Infected epithelial cells tended to be less electron-dense than uninfected cells and were more numer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317902
更新日期：1979-01-01 00:00:00
abstract：:Since the first rescue of a recombinant Newcastle disease virus (rNDV) in the late 1990s, many more rNDVs have been rescued by researchers around the world. Regardless of methodology, the main principle behind rescue of the virus has remained the same, i.e., the formation of a functional replication complex by simulta...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-016-3065-7
更新日期：2017-01-01 00:00:00
abstract：:The VP1 encoded by genomic segment B of birnaviruses is generally known to exist as a genome-linked protein (VPg) and as a "free" polypeptide of 90 kDa in virus particles. The guanylylation activity associated with infectious bursal disease virus (IBDV) was demonstrated by incubating purified virus in presence of [alp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050154
更新日期：1997-01-01 00:00:00
abstract：:We previously generated an RCMV strain in which the r144 gene, encoding a major histocompatibility complex class I homolog, had been deleted (RCMVdelta r144). To investigate the role of r144 during acute infection of neonatal rats, we infected three days-old neonatal rats with either RCMVdelta r144 or wild type (wt) R...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050200028
更新日期：2002-04-01 00:00:00
abstract：:The rate of evolution of the human papillomavirus 16 (HPV16) genome is low. However, the ability of the E6 oncoprotein to interact with distinct p53 variants causes selective pressure on the E6 gene. In addition, intratypic recombination events in the HPV16 E6 and E7 genes have been characterized as extraordinary phen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1555-9
更新日期：2013-04-01 00:00:00
abstract：:A total of 72 Borna Disease virus infected rabbits were treated with different concentrations of cyclophosphamide, glucocorticoids or both in combination. Comparison with untreated, infected rabbits showed a drastic alteration in the clinical picture, a considerable prolongation of the survival time, and differences i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318132
更新日期：1981-01-01 00:00:00
abstract：:The envelope protein of human immunodeficiency virus type 1 (HIV-1) comprises the outer gp 120 SU domain and the anchoring gp41 TM domain, and the conventional view is that it has a single transmembrane region with the following C-terminal sequence situated entirely within the virion. However, we have recently propose...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170199
更新日期：2001-01-01 00:00:00
abstract：:HIV-1 genetic diversity information from a pediatric population is scarce. This study enrolled 128 children living with HIV/AIDS, 103 antiretroviral-treated and 25 naive, from the Sao Paulo metropolitan area. Gag, pol and env regions were amplified, and drug resistance mutations, V3 loop, tropism and viral clades were...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0178-7
更新日期：2008-01-01 00:00:00
abstract：:Hippeastrum chlorotic ringspot virus (HCRV) is a novel tospovirus that was identified in Yunnan Province, China, in 2013. We have sequenced the HCRV L gene, which is 8909 nt long and encodes the RNA-dependent RNA polymerase (2873 amino acids, 330.8 kDa). The HCRV L protein shared highest similarity (89.4 %) with that ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2100-9
更新日期：2014-10-01 00:00:00
abstract：:To investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in Northern Ireland, the ORF5 gene from nine field isolates was sequenced and phylogenetically analysed. The results revealed relatively high diversity amongst isolates, with 87.6-92.2% identity between farms at the nuc...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3886-7
更新日期：2018-10-01 00:00:00
abstract：:Nucleocapsid protein (NP) gene of Newcastle disease virus (NDV) strains mainly isolated in Japan from 1930 to 2001 was genetically characterized. By deduced amino acid sequence comparison, the N-terminal region (from 1 to 401 residues) of the NP protein was found to be highly conserved, while the C-terminal region was...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0309-8
更新日期：2004-08-01 00:00:00
abstract：:A polymerase chain reaction (PCR) based assay capable of detecting and differentiating seven strains of equine arteritis virus (EAV) from around the world was developed. The primers for the PCR were chosen from the ORF6 gene encoding the unglycosylated membrane protein (M). Viral RNA from cell culture fluids infected ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322675
更新日期：1995-01-01 00:00:00
abstract：:Although cell-mediated cytotoxicity effectively kills target virus-infected cells, no careful consideration has been given to the fate of infectious progeny virus contained within target cells following the cytolytic event. To address this issue with respect to murine cytomegalovirus (MCMV) pathogenesis, we developed ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-004-0459-8
更新日期：2005-04-01 00:00:00
abstract：:The betaherpesvirus human herpesvirus 6 (HHV-6) has two variants. The U83 gene product of strain HST is a chemoattractant for monocytes. Here, we describe U83 gene variations that accumulated in variants A and B. A gene-variation hot spot was examined in 36 different strains and one donor DNA sample. U83 gene variatio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0307-3
更新日期：2009-01-01 00:00:00
abstract：:Dengue and chikungunya viruses co-circulate and cause infections that start with similar symptoms but progress to radically different outcomes. Therefore, an early diagnostic test that can differentiate between the two is needed. A single-step multiplex real-time RT-PCR assay was developed that can simultaneously dete...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2217-x
更新日期：2015-01-01 00:00:00
abstract：:Chilo iridescent virus (CIV; the type strain of the genus Iridovirus) replicates productively in larvae of the boll weevil, Anthonomus grandis. This study focuses on characterizing productive infections of a boll weevil cell line, BRL-AG-3A (AG3A), starting with CIV reared in the waxworm, Galleria mellonella. We show ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1417-5
更新日期：2012-11-01 00:00:00
abstract：:In order to determine the prevalence of multiple infections with hepatitis viruses in chronic HBsAg carriers in Naples, to assess the interaction between HBV, HDV and HCV infections and to evaluate the influence of multiple virus hepatitis infections on the clinical presentation, we studied 198 HBsAg chronic carriers ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050090
更新日期：1997-01-01 00:00:00
abstract：:Morphological studies of EIAV reveal knobs on the surface of the particles, conically and tubularly shaped cores, budding particles with dense crescents directly underlying the plasma membrane, and distinct intracytoplasmic structures in infected cells. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315055
更新日期：1977-01-01 00:00:00
abstract：:Virological surveillance is an important element in the Polio Eradication Initiative to provide information rapidly about circulating wild polioviruses. Molecular tools have been developed to identify the serotype of the poliovirus strains and whether they are of vaccine or wild origin (intratypic differentiation) and...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0001-x
更新日期：2008-01-01 00:00:00
abstract：:We investigated the potential of Nicotiana benthamiana to express the E7 protein of human papillomavirus 8 (HPV-8), a paradigm genotype among cutaneous HPVs. The protein, modified in its putative pRb-binding domain (E7(QGD)), was transiently expressed in leaves following infiltration with agrobacteria carrying either ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0893-8
更新日期：2011-04-01 00:00:00
abstract：:Morphological, biological, serological, and molecular tests underpin the description of costus stripe mosaic virus (CoSMV) as a new member of the genus Potyvirus, family Potyviridae. Found affecting the native ornamental Costus spiralis in Brazil, the pathogen showed a severely restricted natural and experimental host...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04788-z
更新日期：2020-11-01 00:00:00
