Identification and analysis of the simian varicella virus thymidine kinase gene.


:The thymidine kinase (TK) of herpesviruses, in contrast to cellular TKs, phosphorylates a variety of substrates including antiherpetic nucleoside analogues. This study reports the identification and DNA sequence of the simian varicella virus (SVV) TK gene. A 32P-labeled varicella zoster virus (VZV) TK DNA probe hybridized to the HindIII B subclone of the SVV BamHI B restriction endonuclease (RE) fragment, indicating the presence of a SVV DNA sequence homologous to the VZV TK gene. DNA sequence analysis of the SVV HindIII B subclone revealed a 1014 base pair (bp) open reading frame (ORF) encoding a 337 amino acid polypeptide homologous to herpesvirus TKs. The predicted SVV and VZV TK polypeptides share 51.3% identity, and alignment of the putative protein sequence of several TK homologues suggests the position of a conserved nucleotide binding site and a nucleoside (substrate) binding site in the SVV TK. Identification of the 5' end of the SVV TK transcript by primer extension analysis allowed a comparison of the SVV and VZV TK promoter regions indicating extensive conservation of the DNA sequence and transcription factor binding sites. Plaque reduction assays demonstrate that the SVV TK is active based on the susceptibility of SVV to acyclovir treatment and that SVV is less sensitive to acyclovir than VZV and herpes simplex virus (HSV-1) in infected Vero cells. Identification of the SVV TK ORF will facilitate studies that examine the role of viral TKs in pathogenesis and antiviral sensitivity and provides a potential insertion site for the expression of foreign genes.


Arch Virol


Archives of virology


Pumphrey CY,Gray WL




Has Abstract


1996-01-01 00:00:00












  • Isolation of two Chinese bovine enteroviruses and sequence analysis of their complete genomes.

    abstract::In this study, RNA corresponding to bovine enterovirus (BEV) was detected in 24.6 % of faecal samples (17/69) from diarrheic and healthy cattle in six different areas in China by an RT-PCR screening method. Furthermore, two cytopathic agents, designated as BHM26 and BJ50, were isolated from the bovine diarrheic fecal ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Li Y,Chang J,Wang Q,Yu L

    更新日期:2012-12-01 00:00:00

  • Comparative sequence analysis of open reading frames 2 to 7 of the modified live vaccine virus and other North American isolates of the porcine reproductive and respiratory syndrome virus.

    abstract::To elucidate changes associated with the attenuated virulence in a modified live porcine reproductive and respiratory syndrome (PRRS) vaccine (Boehringer Ingelheim Animal Health, St. Joseph, MO), derived from an American prototype ATCC virus VR-2332, nucleotide sequence of 3' genome covering open reading frames (ORFs)...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Yang SX,Kwang J,Laegreid W

    更新日期:1998-01-01 00:00:00

  • Abrupt emergence and predominance in Vietnam of rotavirus A strains possessing a bovine-like G8 on a DS-1-like background.

    abstract::An apparently single rotavirus A strain possessing a genotype constellation of G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 abruptly emerged, caused diarrhoea in children requiring hospitalisation, and increased to reach 27 % of strains detected during the first half of 2015 in Vietnam. ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Hoa-Tran TN,Nakagomi T,Vu HM,Do LP,Gauchan P,Agbemabiese CA,Nguyen TT,Nakagomi O,Thanh NT

    更新日期:2016-02-01 00:00:00

  • Detection of antibodies specific for foot-and-mouth disease virus infection using indirect ELISA based on recombinant nonstructural protein 2B.

    abstract::Foot-and-mouth disease (FMD) is a highly contagious viral disease of transboundary importance. In India, since the launch of the FMD control programme, there has been a substantial increase in the vaccinated bovine population. In this scenario, there is a need for additional locally developed non-structural protein (N...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Biswal JK,Jena S,Mohapatra JK,Bisht P,Pattnaik B

    更新日期:2014-07-01 00:00:00

  • Phylogenetic analysis of porcine reproductive and respiratory syndrome virus isolates from Northern Ireland.

    abstract::To investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in Northern Ireland, the ORF5 gene from nine field isolates was sequenced and phylogenetically analysed. The results revealed relatively high diversity amongst isolates, with 87.6-92.2% identity between farms at the nuc...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Smith N,Power UF,McKillen J

    更新日期:2018-10-01 00:00:00

  • Immunisation with DNA polynucleotides protects mice against lethal challenge with St. Louis encephalitis virus.

    abstract::In vivo transfection by intramuscular injection with plasmids expressing the immunogenic proteins of microbial pathogens has considerable potential as a vaccination strategy against many pathogens of both man and animals. Here we report that weanling mice given a single intramuscular injection of 50 micrograms of a pl...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Phillpotts RJ,Venugopal K,Brooks T

    更新日期:1996-01-01 00:00:00

  • Establishment of transformed swine fibroblast cell lines using SV40 large T antigen.

    abstract::Swine testicle cell lines were established by transformation of primary swine testicle (PST) cells with an SV40 plasmid (pSV3-neo), which contains genes conferring resistance to neomycin and expressing SV40 large T antigen. Plasmid DNA was transfected into PST cells using a lipofection system. Two related plasmids, pS...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Choi CS,Murtaugh MP,Molitor TW

    更新日期:1990-01-01 00:00:00

  • Complete genome sequences of two divergent isolates of strawberry crinkle virus coinfecting a single strawberry plant.

    abstract::Strawberry crinkle disease is one of the major diseases that threatens strawberry production. Although the biological properties of the agent, strawberry crinkle virus (SCV), have been thoroughly investigated, its complete genome sequence has never been published. Existing RT-PCR-based detection relies on a partial se...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Koloniuk I,Fránová J,Sarkisova T,Přibylová J

    更新日期:2018-09-01 00:00:00

  • Exposure to watermelon bud necrosis virus and groundnut bud necrosis virus alters the life history traits of their vector, Thrips palmi (Thysanoptera: Thripidae).

    abstract::Thrips palmi transmits the tospoviruses watermelon bud necrosis (WBNV) and groundnut bud necrosis virus (GBNV) in persistent propagative way. Little is known about the T. palmi-WBNV and -GBNV relationship. In this study, we report the effects of WBNV and GBNV infection on the life history traits of T. palmi. Both WBNV...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Ghosh A,Basavaraj YB,Jangra S,Das A

    更新日期:2019-11-01 00:00:00

  • R18C is a new viable P2-like bacteriophage of rabbit origin infecting Citrobacter rodentium and Shigella sonnei strains.

    abstract::Here, we report a novel virulent P2-like bacteriophage, R18C, isolated from rabbit faeces, which, in addition to Escherichia coli K-12 strains, was able to be propagated on Citrobacter rodentium strain ICC169 and a range of Shigella sonnei strains with high efficiency of plating (EOP). It represents the first lytic ba...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Sváb D,Horváth B,Rohde M,Maróti G,Tóth I

    更新日期:2019-12-01 00:00:00

  • Complete genome sequences of two novel begomoviruses infecting common bean in Venezuela.

    abstract::The complete genome sequences of isolates of two new bipartite begomoviruses (genus Begomovirus, family Geminiviridae) found infecting common bean in Venezuela are provided. The names proposed for each of these viruses are "bean yellow chlorosis virus" (BYCV) and "bean white chlorosis mosaic virus" (BWCMV). Phylogenet...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Fiallo-Olivé E,Márquez-Martín B,Hassan I,Chirinos DT,Geraud-Pouey F,Navas-Castillo J,Moriones E

    更新日期:2013-03-01 00:00:00

  • Characterization of R peptide of murine leukemia virus envelope glycoproteins in syncytium formation and entry.

    abstract::The C-terminal R peptide of ecotropic murine leukemia virus (MLV) envelope protein (Env) negatively controls membrane fusion activity. The R peptide cleavage during virion maturation activates its fusogenicity and is required for viral entry. We analyzed fusogenicity and transduction efficiency of mutant Env proteins ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Kubo Y,Tominaga C,Yoshii H,Kamiyama H,Mitani C,Amanuma H,Yamamoto N

    更新日期:2007-01-01 00:00:00

  • Isolation of a recombinant influenza virus (Hsw 1 N2) from swine in Japan.

    abstract::Outbreaks of swine influenza were first observed in Japan in 1978. A number of influenza viruses were isolated from diseased swine. Almost all viruses isolated were swine influenza virus (Hsw 1 N1) but two viruses isolated from the nasal swabs of swine showing clinical signs of influenza in the Kanagawa prefecture wer...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Sugimura T,Yonemochi H,Ogawa T,Tanaka Y,Kumagai T

    更新日期:1980-01-01 00:00:00

  • Characterisation of an isolate of Narcissus degeneration virus from Chinese narcissus (Narcissus tazetta var. chinensis).

    abstract::A potyvirus from Chinese narcissus was transmitted mechanically to three species of Narcissus and to Lycoris radiata but not to 22 other test species. In western blot, the coat protein reacted strongly with Narcissus degeneration virus (UK isolate) antiserum. Antiserum raised to the Chinese virus did not react with ei...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Chen J,Shi YH,Adams MJ,Zheng HY,Qin BX,Chen JP

    更新日期:2007-02-01 00:00:00

  • Molecular characterization of human respiratory syncytial virus, 2010-2011: identification of genotype ON1 and a new subgroup B genotype in Thailand.

    abstract::This study reports the molecular epidemiology and genetic characterization of human respiratory syncytial virus (RSV) samples collected in Thailand from January 2010 to December 2011. In total, 1,315 clinical samples were collected from Bangkok and Khon Kaen provinces and were screened by semi-nested PCR for RSV infec...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Auksornkitti V,Kamprasert N,Thongkomplew S,Suwannakarn K,Theamboonlers A,Samransamruajkij R,Poovorawan Y

    更新日期:2014-03-01 00:00:00

  • A clinical isolate of dengue virus and its proteins induce apoptosis in HMEC-1 cells: a possible implication in pathogenesis.

    abstract::Cumulative studies have demonstrated that dengue virus infection results in the induction of apoptosis of certain cells in vitro. Moreover, apoptosis of microvascular endothelial cells in the brain and in the intestinal serosa has been demonstrated postmortem in dengue virus (DENV)-infected patients. In this work, hum...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Vásquez Ochoa M,García Cordero J,Gutiérrez Castañeda B,Santos Argumedo L,Villegas Sepúlveda N,Cedillo Barrón L

    更新日期:2009-01-01 00:00:00

  • Guinea pig cytomegalovirus: transplacental transmission. Brief report.

    abstract::A well characterized strain of guinea pig cytomegalovirus (GPCMV) was used to infect pregnant guinea pigs during various periods of pregnancy. Transplacental transmission of virus with invasion of the fetus was observed, even in some mothers with preinoculation evidence of GPCMV antibody. Fetal infection occurred duri...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Johnson KP,Connor WS

    更新日期:1979-01-01 00:00:00

  • Three genetic grapevine leafroll-associated virus 3 variants identified from South African vineyards show high variability in their 5'UTR.

    abstract::Three genetic variants of grapevine leafroll-associated virus 3 (GLRaV-3) were identified in vineyards of the Western Cape, South Africa. The GLRaV-3 variants were identified by single-strand conformation polymorphism (SSCP) profiles generated from a region amplified in ORF5. ORF5 sequence data confirmed the three gen...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Jooste AE,Maree HJ,Bellstedt DU,Goszczynski DE,Pietersen G,Burger JT

    更新日期:2010-12-01 00:00:00

  • Rapid visual detection of lily mottle virus using a loop-mediated isothermal amplification method.

    abstract::Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMo...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Zhao B,Yang D,Zhang Y,Xu Y,Zhao X,Liang J,Fan X,Du Y,Zhu Z,Shi B,Zhang Q,Zhang X,Cai Y,Zhao K

    更新日期:2018-02-01 00:00:00

  • Prevalence and natural host range of Homalodisca coagulata virus-1 (HoCV-1).

    abstract::Transmission electron microscopy was used to confirm the presence of picorna-like virus particles presumed to be Homalodisca coagulata virus-1 (HoCV-1) in the midgut region of adult glassy-winged sharpshooters (GWSS). In addition, we offer a reverse transcription polymerase chain reaction (RT-PCR) assay for the detect...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Hunnicutt LE,Mozoruk J,Hunter WB,Crosslin JM,Cave RD,Powell CA

    更新日期:2008-01-01 00:00:00

  • Molecular characterization of CEVd strains that induce different phenotypes in Gynura aurantiaca: structure-pathogenicity relationships.

    abstract::Two Citrus exocortis viroid isolates (CEVd-s and CEVd-129) that induce severe and mild symptoms in Gynura aurantiaca, respectively, have been characterized. They present nucleotide sequences in the pathogenicity motifs P(L), C and P(R) similar to those of "Class A" and "Class B". Infectivity and symptom expression in ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Chaffai M,Serra P,Gandía M,Hernández C,Duran-Vila N

    更新日期:2007-01-01 00:00:00

  • Cell membrane bound N-acetylneuraminic acid is involved in the infection of fibroblasts and phorbol-ester differentiated monocyte-like cells with human cytomegalovirus (HCMV).

    abstract::We focused on the role of membrane bound sugar residues in the infection of fibroblasts and monocyte-like cells with human cytomegalovirus (HCMV). Treatment of phorbol 12-myristate 13-acetate (PMA) differentiated monocyte-like cells THP-1 or human fibroblasts MRC-5 with lectins specific for N-acetylneuraminic acid (Ne...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Lobert PE,Hober D,Dewilde A,Wattré P

    更新日期:1995-01-01 00:00:00

  • Coat protein gene sequence of an Austrian isolate of grapevine fanleaf virus.

    abstract::The nucleotide sequence of the coat protein cistron of an Austrian isolate of grapevine fanleaf virus (GFLV-FC) from Vitis vinifera cv. French Colombard was determined. It shows small differences at the RNA level as well as at the protein level compared to the sequences of already published grapevine fanleaf virus str...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Brandt S,Ibl M,Himmler G

    更新日期:1995-01-01 00:00:00

  • The effects of gamma interferon on replication of foot-and-mouth disease virus in persistently infected bovine cells.

    abstract::Foot-and-mouth disease virus (FMDV) causes a highly contagious viral disease of cloven-hoofed animals, which has a considerable socio-economic impact on the countries affected. In addition, persistent infection can occur following clinical or sub-clinical disease in either vaccinated or non-vaccinated cattle. The mech...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Zhang ZD,Hutching G,Kitching P,Alexandersen S

    更新日期:2002-11-01 00:00:00

  • Modes of Seoul virus infections: persistency in newborn rats and transiency in adult rats.

    abstract::To understand the mode of persistent infection of Seoul virus in rodents, we examined the distribution of the virus genome and antibody production in infected rats. When 1-day-old rats were inoculated with the KI-83-262 strain, the S segment of viral genome was detected in sera, clots, lungs and kidneys from 3 to 184 ...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Kariwa H,Kimura M,Yoshizumi S,Arikawa J,Yoshimatsu K,Takashima I,Hashimoto N

    更新日期:1996-01-01 00:00:00

  • Computational analysis of the effect of polymerase acidic (PA) gene mutation F35L in the 2009 pandemic influenza A (H1N1) virus on binding aspects of mononucleotides in the endonuclease domain.

    abstract::An F35L mutation in the N-terminal domain of the polymerase acidic protein (PA-Nter), which contains the active site of the endonuclease, has been reported to result in higher polymerase activity in mouse-adapted strains of the 2009 pandemic influenza A H1N1 virus. We modeled wild and mutant complexes of uridine 5'-mo...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Bhoye D,Cherian SS

    更新日期:2018-04-01 00:00:00

  • Molecular characterization of Lolium latent virus, proposed type member of a new genus in the family Flexiviridae.

    abstract::Lolium latent virus (LoLV) was recently detected in the USA for the first time in ryegrass hybrids (Lolium perenne x Lolium multiflorum). The genome of one USA isolate, LoLV-US1, has now been fully sequenced. The positive strand genomic RNA is 7674 nucleotides (nt) long and is organized in five open reading frames (OR...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Vaira AM,Maroon-Lango CJ,Hammond J

    更新日期:2008-01-01 00:00:00

  • A new sequence variant of Coleus blumei viroid 1 from the Coleus blumei cultivar "Rainbow Gold'.

    abstract::A viroid was isolated from symptomless Coleus blumei cultivar (cv.) 'Rainbow Gold' plants using the bidirectional PAGE method for analysis of small circular RNA molecules. The viriod was transmitted to viroid-free plants of Coleus blumei cv. 'Scarlet Dragonfly' by mechanical inoculation. Cloning and sequencing reveale...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Spieker RL

    更新日期:1996-01-01 00:00:00

  • The role of host immunocompetence in neuroinvasion of Sindbis virus.

    abstract::Viral infections of the central nervous system (CNS) following peripheral inoculation of Sindbis viruses were studied. The use of viral strains, which vary in their neuroinvasive and neurovirulent properties, and various strains of mice, which differ in immunocompetence, revealed several pathways of viral neuroinvasio...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Lustig S,Halevy M,Ben-Nathan D,Rice CM,Kobiler D

    更新日期:1999-01-01 00:00:00

  • Phylogenetic and recombination analysis of rice black-streaked dwarf virus segment 9 in China.

    abstract::Rice black-streaked dwarf virus (RBSDV) is an economically important virus that causes maize rough dwarf disease and rice black-streaked dwarf disease in East Asia. To study RBSDV variation and recombination, we examined the segment 9 (S9) sequences of 49 RBSDV isolates from maize and rice in China. Three S9 recombina...

    journal_title:Archives of virology

    pub_type: 杂志文章


    authors: Zhou Y,Weng JF,Chen YP,Liu CL,Han XH,Hao ZF,Li MS,Yong HJ,Zhang DG,Zhang SH,Li XH

    更新日期:2015-04-01 00:00:00