Abstract：
:The thymidine kinase (TK) of herpesviruses, in contrast to cellular TKs, phosphorylates a variety of substrates including antiherpetic nucleoside analogues. This study reports the identification and DNA sequence of the simian varicella virus (SVV) TK gene. A 32P-labeled varicella zoster virus (VZV) TK DNA probe hybridized to the HindIII B subclone of the SVV BamHI B restriction endonuclease (RE) fragment, indicating the presence of a SVV DNA sequence homologous to the VZV TK gene. DNA sequence analysis of the SVV HindIII B subclone revealed a 1014 base pair (bp) open reading frame (ORF) encoding a 337 amino acid polypeptide homologous to herpesvirus TKs. The predicted SVV and VZV TK polypeptides share 51.3% identity, and alignment of the putative protein sequence of several TK homologues suggests the position of a conserved nucleotide binding site and a nucleoside (substrate) binding site in the SVV TK. Identification of the 5' end of the SVV TK transcript by primer extension analysis allowed a comparison of the SVV and VZV TK promoter regions indicating extensive conservation of the DNA sequence and transcription factor binding sites. Plaque reduction assays demonstrate that the SVV TK is active based on the susceptibility of SVV to acyclovir treatment and that SVV is less sensitive to acyclovir than VZV and herpes simplex virus (HSV-1) in infected Vero cells. Identification of the SVV TK ORF will facilitate studies that examine the role of viral TKs in pathogenesis and antiviral sensitivity and provides a potential insertion site for the expression of foreign genes.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Pumphrey CY,Gray WLdoi
10.1007/BF01718587subject
Has Abstractpub_date
1996-01-01 00:00:00pages
43-55issue
1eissn
0304-8608issn
1432-8798journal_volume
141pub_type
杂志文章abstract：:In this study, RNA corresponding to bovine enterovirus (BEV) was detected in 24.6 % of faecal samples (17/69) from diarrheic and healthy cattle in six different areas in China by an RT-PCR screening method. Furthermore, two cytopathic agents, designated as BHM26 and BJ50, were isolated from the bovine diarrheic fecal ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1424-6
更新日期：2012-12-01 00:00:00
abstract：:To elucidate changes associated with the attenuated virulence in a modified live porcine reproductive and respiratory syndrome (PRRS) vaccine (Boehringer Ingelheim Animal Health, St. Joseph, MO), derived from an American prototype ATCC virus VR-2332, nucleotide sequence of 3' genome covering open reading frames (ORFs)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050316
更新日期：1998-01-01 00:00:00
abstract：:An apparently single rotavirus A strain possessing a genotype constellation of G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 abruptly emerged, caused diarrhoea in children requiring hospitalisation, and increased to reach 27 % of strains detected during the first half of 2015 in Vietnam. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2682-x
更新日期：2016-02-01 00:00:00
abstract：:Foot-and-mouth disease (FMD) is a highly contagious viral disease of transboundary importance. In India, since the launch of the FMD control programme, there has been a substantial increase in the vaccinated bovine population. In this scenario, there is a need for additional locally developed non-structural protein (N...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1973-3
更新日期：2014-07-01 00:00:00
abstract：:To investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in Northern Ireland, the ORF5 gene from nine field isolates was sequenced and phylogenetically analysed. The results revealed relatively high diversity amongst isolates, with 87.6-92.2% identity between farms at the nuc...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3886-7
更新日期：2018-10-01 00:00:00
abstract：:In vivo transfection by intramuscular injection with plasmids expressing the immunogenic proteins of microbial pathogens has considerable potential as a vaccination strategy against many pathogens of both man and animals. Here we report that weanling mice given a single intramuscular injection of 50 micrograms of a pl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718332
更新日期：1996-01-01 00:00:00
abstract：:Swine testicle cell lines were established by transformation of primary swine testicle (PST) cells with an SV40 plasmid (pSV3-neo), which contains genes conferring resistance to neomycin and expressing SV40 large T antigen. Plasmid DNA was transfected into PST cells using a lipofection system. Two related plasmids, pS...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310532
更新日期：1990-01-01 00:00:00
abstract：:Strawberry crinkle disease is one of the major diseases that threatens strawberry production. Although the biological properties of the agent, strawberry crinkle virus (SCV), have been thoroughly investigated, its complete genome sequence has never been published. Existing RT-PCR-based detection relies on a partial se...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3860-4
更新日期：2018-09-01 00:00:00
abstract：:Thrips palmi transmits the tospoviruses watermelon bud necrosis (WBNV) and groundnut bud necrosis virus (GBNV) in persistent propagative way. Little is known about the T. palmi-WBNV and -GBNV relationship. In this study, we report the effects of WBNV and GBNV infection on the life history traits of T. palmi. Both WBNV...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04381-z
更新日期：2019-11-01 00:00:00
abstract：:Here, we report a novel virulent P2-like bacteriophage, R18C, isolated from rabbit faeces, which, in addition to Escherichia coli K-12 strains, was able to be propagated on Citrobacter rodentium strain ICC169 and a range of Shigella sonnei strains with high efficiency of plating (EOP). It represents the first lytic ba...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04424-5
更新日期：2019-12-01 00:00:00
abstract：:The complete genome sequences of isolates of two new bipartite begomoviruses (genus Begomovirus, family Geminiviridae) found infecting common bean in Venezuela are provided. The names proposed for each of these viruses are "bean yellow chlorosis virus" (BYCV) and "bean white chlorosis mosaic virus" (BWCMV). Phylogenet...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1545-y
更新日期：2013-03-01 00:00:00
abstract：:The C-terminal R peptide of ecotropic murine leukemia virus (MLV) envelope protein (Env) negatively controls membrane fusion activity. The R peptide cleavage during virion maturation activates its fusogenicity and is required for viral entry. We analyzed fusogenicity and transduction efficiency of mutant Env proteins ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1054-6
更新日期：2007-01-01 00:00:00
abstract：:Outbreaks of swine influenza were first observed in Japan in 1978. A number of influenza viruses were isolated from diseased swine. Almost all viruses isolated were swine influenza virus (Hsw 1 N1) but two viruses isolated from the nasal swabs of swine showing clinical signs of influenza in the Kanagawa prefecture wer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314741
更新日期：1980-01-01 00:00:00
abstract：:A potyvirus from Chinese narcissus was transmitted mechanically to three species of Narcissus and to Lycoris radiata but not to 22 other test species. In western blot, the coat protein reacted strongly with Narcissus degeneration virus (UK isolate) antiserum. Antiserum raised to the Chinese virus did not react with ei...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0841-9
更新日期：2007-02-01 00:00:00
abstract：:This study reports the molecular epidemiology and genetic characterization of human respiratory syncytial virus (RSV) samples collected in Thailand from January 2010 to December 2011. In total, 1,315 clinical samples were collected from Bangkok and Khon Kaen provinces and were screened by semi-nested PCR for RSV infec...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-013-1773-9
更新日期：2014-03-01 00:00:00
abstract：:Cumulative studies have demonstrated that dengue virus infection results in the induction of apoptosis of certain cells in vitro. Moreover, apoptosis of microvascular endothelial cells in the brain and in the intestinal serosa has been demonstrated postmortem in dengue virus (DENV)-infected patients. In this work, hum...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0396-7
更新日期：2009-01-01 00:00:00
abstract：:A well characterized strain of guinea pig cytomegalovirus (GPCMV) was used to infect pregnant guinea pigs during various periods of pregnancy. Transplacental transmission of virus with invasion of the fetus was observed, even in some mothers with preinoculation evidence of GPCMV antibody. Fetal infection occurred duri...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317422
更新日期：1979-01-01 00:00:00
abstract：:Three genetic variants of grapevine leafroll-associated virus 3 (GLRaV-3) were identified in vineyards of the Western Cape, South Africa. The GLRaV-3 variants were identified by single-strand conformation polymorphism (SSCP) profiles generated from a region amplified in ORF5. ORF5 sequence data confirmed the three gen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0793-y
更新日期：2010-12-01 00:00:00
abstract：:Lily mottle virus (LMoV; genus Potyvirus, family Potyviridae) infects plants of the genus Lilium, causing a reduction in flower and bulb quality. A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay was developed to detect the coat protein gene of LMoV. This LAMP method was highly specific for LMo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3618-4
更新日期：2018-02-01 00:00:00
abstract：:Transmission electron microscopy was used to confirm the presence of picorna-like virus particles presumed to be Homalodisca coagulata virus-1 (HoCV-1) in the midgut region of adult glassy-winged sharpshooters (GWSS). In addition, we offer a reverse transcription polymerase chain reaction (RT-PCR) assay for the detect...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1066-2
更新日期：2008-01-01 00:00:00
abstract：:Two Citrus exocortis viroid isolates (CEVd-s and CEVd-129) that induce severe and mild symptoms in Gynura aurantiaca, respectively, have been characterized. They present nucleotide sequences in the pathogenicity motifs P(L), C and P(R) similar to those of "Class A" and "Class B". Infectivity and symptom expression in ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0958-5
更新日期：2007-01-01 00:00:00
abstract：:We focused on the role of membrane bound sugar residues in the infection of fibroblasts and monocyte-like cells with human cytomegalovirus (HCMV). Treatment of phorbol 12-myristate 13-acetate (PMA) differentiated monocyte-like cells THP-1 or human fibroblasts MRC-5 with lectins specific for N-acetylneuraminic acid (Ne...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322663
更新日期：1995-01-01 00:00:00
abstract：:The nucleotide sequence of the coat protein cistron of an Austrian isolate of grapevine fanleaf virus (GFLV-FC) from Vitis vinifera cv. French Colombard was determined. It shows small differences at the RNA level as well as at the protein level compared to the sequences of already published grapevine fanleaf virus str...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309730
更新日期：1995-01-01 00:00:00
abstract：:Foot-and-mouth disease virus (FMDV) causes a highly contagious viral disease of cloven-hoofed animals, which has a considerable socio-economic impact on the countries affected. In addition, persistent infection can occur following clinical or sub-clinical disease in either vaccinated or non-vaccinated cattle. The mech...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0867-6
更新日期：2002-11-01 00:00:00
abstract：:To understand the mode of persistent infection of Seoul virus in rodents, we examined the distribution of the virus genome and antibody production in infected rats. When 1-day-old rats were inoculated with the KI-83-262 strain, the S segment of viral genome was detected in sera, clots, lungs and kidneys from 3 to 184 ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718634
更新日期：1996-01-01 00:00:00
abstract：:An F35L mutation in the N-terminal domain of the polymerase acidic protein (PA-Nter), which contains the active site of the endonuclease, has been reported to result in higher polymerase activity in mouse-adapted strains of the 2009 pandemic influenza A H1N1 virus. We modeled wild and mutant complexes of uridine 5'-mo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3681-x
更新日期：2018-04-01 00:00:00
abstract：:Lolium latent virus (LoLV) was recently detected in the USA for the first time in ryegrass hybrids (Lolium perenne x Lolium multiflorum). The genome of one USA isolate, LoLV-US1, has now been fully sequenced. The positive strand genomic RNA is 7674 nucleotides (nt) long and is organized in five open reading frames (OR...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0108-8
更新日期：2008-01-01 00:00:00
abstract：:A viroid was isolated from symptomless Coleus blumei cultivar (cv.) 'Rainbow Gold' plants using the bidirectional PAGE method for analysis of small circular RNA molecules. The viriod was transmitted to viroid-free plants of Coleus blumei cv. 'Scarlet Dragonfly' by mechanical inoculation. Cloning and sequencing reveale...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718222
更新日期：1996-01-01 00:00:00
abstract：:Viral infections of the central nervous system (CNS) following peripheral inoculation of Sindbis viruses were studied. The use of viral strains, which vary in their neuroinvasive and neurovirulent properties, and various strains of mice, which differ in immunocompetence, revealed several pathways of viral neuroinvasio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050576
更新日期：1999-01-01 00:00:00
abstract：:Rice black-streaked dwarf virus (RBSDV) is an economically important virus that causes maize rough dwarf disease and rice black-streaked dwarf disease in East Asia. To study RBSDV variation and recombination, we examined the segment 9 (S9) sequences of 49 RBSDV isolates from maize and rice in China. Three S9 recombina...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2291-0
更新日期：2015-04-01 00:00:00
