Abstract:
:The thymidine kinase (TK) of herpesviruses, in contrast to cellular TKs, phosphorylates a variety of substrates including antiherpetic nucleoside analogues. This study reports the identification and DNA sequence of the simian varicella virus (SVV) TK gene. A 32P-labeled varicella zoster virus (VZV) TK DNA probe hybridized to the HindIII B subclone of the SVV BamHI B restriction endonuclease (RE) fragment, indicating the presence of a SVV DNA sequence homologous to the VZV TK gene. DNA sequence analysis of the SVV HindIII B subclone revealed a 1014 base pair (bp) open reading frame (ORF) encoding a 337 amino acid polypeptide homologous to herpesvirus TKs. The predicted SVV and VZV TK polypeptides share 51.3% identity, and alignment of the putative protein sequence of several TK homologues suggests the position of a conserved nucleotide binding site and a nucleoside (substrate) binding site in the SVV TK. Identification of the 5' end of the SVV TK transcript by primer extension analysis allowed a comparison of the SVV and VZV TK promoter regions indicating extensive conservation of the DNA sequence and transcription factor binding sites. Plaque reduction assays demonstrate that the SVV TK is active based on the susceptibility of SVV to acyclovir treatment and that SVV is less sensitive to acyclovir than VZV and herpes simplex virus (HSV-1) in infected Vero cells. Identification of the SVV TK ORF will facilitate studies that examine the role of viral TKs in pathogenesis and antiviral sensitivity and provides a potential insertion site for the expression of foreign genes.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Pumphrey CY,Gray WLdoi
10.1007/BF01718587subject
Has Abstractpub_date
1996-01-01 00:00:00pages
43-55issue
1eissn
0304-8608issn
1432-8798journal_volume
141pub_type
杂志文章abstract::An alpha-herpesvirus has recently been associated with green turtle fibropapilloma (FP). To further understand the etiological role of this newfound green turtle herpesvirus (GTHV) in the pathogenesis of FP, expression of GTHV polymerase ( pol) gene was determined in tumors and normal-appearing nontumor tissues and or...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0970-8
更新日期:2003-06-01 00:00:00
abstract::Trigeminal ganglia from swine latently infected with pseudorabies virus (PRV) were examined for the presence of PRV nucleic acids by in situ hybridization. Both viral DNA and RNA were detectable in latently infected neurons, indicating that the PRV genome is transcriptionally active during the latent phase of infectio...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01321010
更新日期:1988-01-01 00:00:00
abstract::The temperature sensitivity of herpes simplex virus type 1 (HSV-1) was assessed in primary cultures of mouse central nervous system (MNS) cells and mouse embryo cells (MEC). Infectious yields were determined and the ultrastructural morphogenesis of HSV-1 particles was compared following incubation at 37 or 40.5 degree...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309574
更新日期:1992-01-01 00:00:00
abstract::The envelope protein of human immunodeficiency virus type 1 (HIV-1) comprises the outer gp 120 SU domain and the anchoring gp41 TM domain, and the conventional view is that it has a single transmembrane region with the following C-terminal sequence situated entirely within the virion. However, we have recently propose...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170199
更新日期:2001-01-01 00:00:00
abstract::Wheat streak mosaic virus is a serious threat in wheat-producing countries. In Germany, the virus was first recorded in 2013 near Hoym. The complete sequence of isolate Hoym was obtained and compared to all other known complete WSMV sequences, including newly collected and sequenced isolates from France and Austria. P...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2422-2
更新日期:2015-07-01 00:00:00
abstract::The 3'-terminal nucleotides of the genome segments of influenza C/Taylor/47. C/Bavaria/79 and C/Johannesburg/1/66 were identified by two RNA sequencing techniques. These comprised 11 nucleotides (3' UCGUCUUCGUC) which were found to be conserved among the genome segments of each virus. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310664
更新日期:1984-01-01 00:00:00
abstract::Phages, viruses targeting bacteria, have potential therapeutic applications in the control of infections with antibiotic-resistant bacteria. In this study, an Enterobacter cloacae phage, Ec_L1, was isolated from sewage sludge samples collected from a hospital. The genome of phage Ec_L1 consists of 51,894 bp with 48.24...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04672-w
更新日期:2020-08-01 00:00:00
abstract::In this cross-sectional study, we evaluated the efficiency of the plasma of 38 antiretroviral-naïve HIV-1-infected children from northern India against a standard panel of pseudoviruses (3 clade C and 3 clade B) by TZM-bl assay. Neutralization potential was observed to a variable extent, with a potency ranging up to r...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1357-0
更新日期:2012-09-01 00:00:00
abstract::The in vitro cytopathology and the neurovirulence to inbred mice demonstrated by five variants originally derived from one laboratory strain (Miyama) of type 1 herpes simplex virus (HSV-1) were studied comparatively. Three of the variants are syncytial [+GC (LPV), +GC (SPV), +GC (81)] and two are non-syncytial [-GCr a...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317369
更新日期:1986-01-01 00:00:00
abstract::Our previous studies demonstrated that hepatitis E virus (HEV) requires the multivesicular body (MVB) pathway to release virus particles, suggesting that HEV utilizes the cellular ESCRT machinery in the cytoplasm, not at the cell surface, to be released from infected cells. In this study, we generated a murine monoclo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1912-3
更新日期:2014-05-01 00:00:00
abstract::Infection with West Nile virus and dengue virus, two mosquito-borne flaviviruses, is enhanced by two calcium-dependent lectins: dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), and its related molecule (DC-SIGNR). The present study examined the relationship between Japanese enc...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2042-2
更新日期:2014-08-01 00:00:00
abstract::Antibody and cell-mediated immune responses were measured in rabbits immunized with live, UV inactivated herpes simplex virus or with a subunit vaccine containing envelope proteins. All the types of immunization procedures induced the production of antibody as well as a specific cellular immunity. Furthermore, the sub...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317862
更新日期:1976-01-01 00:00:00
abstract::Little is known about the bacteriophage proteins expressed immediately after infection of the host cell. Most of these early proteins are probably involved in bacteriophage-host interactions redirecting the bacterial metabolism to phage production. Interaction analysis of the first 16 phiKMV gene products (gp) identif...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-0967-4
更新日期:2007-01-01 00:00:00
abstract::Monoclonal antibodies raised against the separated hemagglutinin subunits (HA1 and HA2) of influenza A/Vic/3/75 (H3N2) virus were tested against a large panel of human and avian strains. The epitopes recognized by most antibodies were conserved among subtype H3 viruses, but reactivity of some antibodies with members o...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01319250
更新日期:1991-01-01 00:00:00
abstract::We have applied an RNA:RNA hybridization test for the detection of human coronavirus 229E. This test is undergoing further development but already allows a diagnosis of HCV 229E infection within 48 hours. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315554
更新日期:1989-01-01 00:00:00
abstract::The entry modes of Japanese encephalitis (JE) and dengue-2 (DEN-2) viruses into C6/36 mosquito cells and of DEN-2 virus into human peripheral blood monocytes in vitro were studied. Inoculation of either JE or DEN-2 virions into C6/36 cells resulted in direct penetration of the virions into the cytoplasm at the cell su...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01313814
更新日期:1989-01-01 00:00:00
abstract::Simian adenovirus SA7 heated for 30 minutes at 70 degrees C retained part of its ability to induce tumors in newborn hamsters. Tumors were also induced by DNA extracted from the heated virus. However, neither residual replicating, i.e. cytopathogenic, nor transforming and T-antigen-inducing activities could be detecte...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317334
更新日期:1981-01-01 00:00:00
abstract::The sequence variability of Barley yellow dwarf virus-PAV (PAV) and Barley yellow dwarf virus-MAV (MAV) was studied by comparing 502 nucleotides from the coat protein-encoding region of six isolates from Latvia and four from Sweden. The diversity within MAV was low (>97% sequence identity), also when compared to isola...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0242-2
更新日期:2004-04-01 00:00:00
abstract::In this study, we obtained the whole genomes of three porcine bocaparvovirus (PBoV) strains (GD6, GD10, and GD23) by polymerase chain reaction. Sequence analysis showed that all three field strains belonged to PBoV group 3 (G3). The phylogenetic trees based on NS1, NP1, and VP1 differed to the extent that these PBoVs ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3606-8
更新日期:2018-03-01 00:00:00
abstract::The complete genome sequence of the T4-related low-temperature Escherichia coli bacteriophage vB_EcoM-VR7 was determined. The genome sequence is 169,285 bp long, with a G+C content of 40.3%. Overall, 95% of the phage genome is coding. It encodes 293 putative protein-encoding open reading frames (ORFs) and tRNA(Met). M...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1084-y
更新日期:2011-10-01 00:00:00
abstract::Fragaria (strawberry) and Rubus species (blackberry, wild blackberry, red raspberry and black raspberry) were thought to be infected with distinct isolates of Tobacco streak virus (TSV). Employing serology and nucleic acid hybridization it has been shown that these isolates form a cluster distinct from other strains o...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0330-y
更新日期:2004-10-01 00:00:00
abstract::Noroviruses, a major cause of acute gastroenteritis outbreaks worldwide, are constantly evolving. This ability is reflected in the speed and efficiency with which these viruses spread and remain in the human population. The present study reports the detection of a novel recombination event among norovirus genotypes in...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1428-2
更新日期:2012-12-01 00:00:00
abstract::Alfamo- and ilarviruses are characterized by the deficiency of their genomes (three messenger-sense RNAs) to start an infection cycle. The RNAs are in capsids built from a single species of protein of about 24 kD. A few dimers of this coat protein per RNA molecule are sufficient to activate the genome. Since the first...
journal_title:Archives of virology
pub_type: 杂志文章,评审
doi:10.1007/s007050050551
更新日期:1999-01-01 00:00:00
abstract::The hemagglutinin-esterase (HE) protein of betacoronavirus lineage A is a secondary receptor in the infection process and is involved in the emergence of new betacoronavirus genotypes with altered host specificity and tissue tropism. We previously reported a novel recombinant bovine coronavirus (BCoV) strain that was ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04840-y
更新日期:2020-12-01 00:00:00
abstract::Localization of neutralizing, serotype specific epitopes of infectious bronchitis virus has been difficult because these epitopes are conformationally dependent. We identified amino acids involved in a serotype specific, conformationally dependent epitope by analysis of the S1 gene of 13 monoclonal antibody-neutraliza...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050239
更新日期:1997-01-01 00:00:00
abstract::Truncated proteins corresponding to the C-terminal half of VP1 of four vaccine strains and two field variants of foot-and-mouth disease virus (FMDV) were expressed in E. coli. The expressed proteins were affinity purified and their type specific reactivity was confirmed by immunoprecipitation with anti-virus antibodie...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050698
更新日期:1999-01-01 00:00:00
abstract::We determined the complete nucleotide sequence of a broad bean wilt virus 2 (BBWV-2) isolate from gentian in Japan. The full-length RNA1 and RNA2 sequences, excluding poly(A) tails, were 5955 and 3600 nucleotides long, respectively. Analysis indicated that, in contrast to other BBWV-2 isolates, the 5' end of both RNA1...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1625-7
更新日期:2013-07-01 00:00:00
abstract::Feline calicivirus (FCV) is a highly contagious pathogen that causes oral and upper respiratory tract disease in cats. Despite widespread vaccination, the prevalence of FCV remains high. Furthermore, a high gene mutation rate has led to the emergence of variants, and some infections are lethal. To date, there is no ef...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2534-8
更新日期:2015-12-01 00:00:00
abstract::Several species in the genus Datura (family Solanaceae) are hosts for begomoviruses (family Geminiviridae), both in the New World and the Old World. Here, we report the genome characterization of two isolates of a novel Old World monopartite begomovirus infecting Datura innoxia in Sudan, which we propose to be named "...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3574-z
更新日期:2018-01-01 00:00:00
abstract::A putative novel positive-sense (+) RNA virus was detected in isolate CF16158 of the fungus Fusarium graminearum, the causal agent of Fusarium head blight and crown rot in wheat in China. The full genome of this virus was sequenced and characterized. The complete cDNA sequence is 7,051 nt long and contains four open r...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04486-5
更新日期:2020-02-01 00:00:00