Immunisation with DNA polynucleotides protects mice against lethal challenge with St. Louis encephalitis virus.

Abstract:

:In vivo transfection by intramuscular injection with plasmids expressing the immunogenic proteins of microbial pathogens has considerable potential as a vaccination strategy against many pathogens of both man and animals. Here we report that weanling mice given a single intramuscular injection of 50 micrograms of a plasmid, pSLE1 expressing the St. Louis encephalitis virus (SLE) prM/E protein under the control of the cytomegalovirus immediate early protein promoter produced SLE-specific antibody and were protected against lethal challenge with the virulent virus. Polynucleotide vaccine technology provides a unique opportunity to produce vaccines against flavivirus diseases of low incidence cheaply and rapidly, and to produce multivalent vaccines such as would be required for immunisation against dengue virus disease.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Phillpotts RJ,Venugopal K,Brooks T

doi

10.1007/BF01718332

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

743-9

issue

3-4

eissn

0304-8608

issn

1432-8798

journal_volume

141

pub_type

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