Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan.

Abstract:

:Mycobacteriophage archival stocks have been kept for ca. 20-50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Ujihara T,Uchiyama J,Nasukawa T,Ando H,Murakami H,Ohara N,Ogawa M,Yamazaki T,Daibata M,Sakaguchi M,Matsuzaki S

doi

10.1007/s00705-018-3788-8

subject

Has Abstract

pub_date

2018-07-01 00:00:00

pages

1915-1919

issue

7

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-018-3788-8

journal_volume

163

pub_type

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