Abstract:
:Mycobacteriophage archival stocks have been kept for ca. 20-50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Ujihara T,Uchiyama J,Nasukawa T,Ando H,Murakami H,Ohara N,Ogawa M,Yamazaki T,Daibata M,Sakaguchi M,Matsuzaki Sdoi
10.1007/s00705-018-3788-8subject
Has Abstractpub_date
2018-07-01 00:00:00pages
1915-1919issue
7eissn
0304-8608issn
1432-8798pii
10.1007/s00705-018-3788-8journal_volume
163pub_type
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