The use of agarose gels for the assay of the deoxyribonuclease activity associated with the soluble penton of adenoviruses.

Abstract:

:Evidence is presented that the excess pool of penton complexes purified from adenovirus type 1-infected HEp-2 cells also carry an endonucleolytic activity. The effect was measured with both E. coli and adenovirus type 1 double-stranded DNA substrates. The use of agarose gels for assaying the endonuclease activity allowed the approximative calculation of the number of penton molecules which might produce one DNA double-strand break under the experimental conditions selected.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Medveczky P,Csuzi S,Nász I,Adám E,Berencsi G,Antoni F

doi

10.1007/BF01315620

subject

Has Abstract

pub_date

1976-01-01 00:00:00

pages

315-21

issue

4

eissn

0304-8608

issn

1432-8798

journal_volume

52

pub_type

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