Abstract:
:Utilizing co-transfection of DNA from glycoprotein gB- strain of HSV1 and cloned fragments of several simian alpha-herpesviruses containing the UL26, UL27 (gB glycoprotein), and UL28 gene homologs, replication-competent recombinant viruses were produced. Genetic analysis of one HSV1/SA8 recombinant (HSV1/SgB) demonstrated the presence of SA8 DNA comprising the entire UL27 (gB) gene and parts of the UL28 and UL26 ORFs in an otherwise HSV1 genome. The recombinant was shown to express the SA8 gB and p40 proteins (UL27 & UL26.5 gene products, respectively); all other proteins were indistinguishable from those of HSV1. The recombinant behaved like SA8 in gB-specific virus neutralization and cell surface antibody binding assays, while plaque morphology and replication kinetics were very similar to HSV1. Despite its overwhelming HSV1 genetic constitution, the recombinant displayed a pathogenic phenotype in mice very different from the parental HSV1. While HSV1 produced corneal disease in ocularly infected mice and readily spread to the nervous system. HSV1/SgB was markedly impaired in both respects. These results demonstrate the functional equivalency of the cercopithecine monkey virus gB glycoproteins and genes (including transcriptional regulatory elements) in HSV1, the functional nature of HSV1/SA8 chimeric UL28 and UL26 genes/proteins, and that UL28, gB and/or p40 proteins may effect the pathogenicity of HSV1.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Eberle R,Tanamachi B,Black D,Blewett EL,Ali M,Openshaw H,Cantin EMdoi
10.1007/s007050050114subject
Has Abstractpub_date
1997-01-01 00:00:00pages
721-36issue
4eissn
0304-8608issn
1432-8798journal_volume
142pub_type
杂志文章abstract::Non-neoplastic tonsils were analyzed for detection of Epstein-Barr virus (EBV)-positive cells by in situ hybridization and in situ PCR. EBV-encoded small nuclear RNA 1(EBER1)-positive cells were found in 28.2% of the tonsils and were evenly localized in the extrafollicular area and within germinal centers. Latent memb...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050332
更新日期:1998-01-01 00:00:00
abstract::Beet necrotic yellow vein virus RNA 1 contains a single long ORF corresponding to the theoretical translation product of 237 kDa which contains the information necessary for replication of the viral genome. This ORF contains a putative papain-like proteinase domain which has been localized, on the basis of sequence al...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050141
更新日期:1997-01-01 00:00:00
abstract::RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA speci...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1166-x
更新日期:2012-02-01 00:00:00
abstract::The '6K1' protein of the Pinellia isolate of Soybean mosaic virus was cloned into a prokaryotic expression vector and a polyclonal antiserum raised to the expressed fusion protein. In immunogold labeling of thin sections of infected leaves of Pinellia ternata, specific labeling occurred at the cell periphery. This mig...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-007-0972-7
更新日期:2007-01-01 00:00:00
abstract::Influenza C viruses did not possess neuraminidase activity when examined using either fetuin or sialyllactose as substrate. Purified preparations of influenza C virus inhibited hemagglutination by NWS hemagglutinin. The hemagglutination inhibiting activity was abolished by treatment of influenza C virus with neuramini...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01320578
更新日期:1976-01-01 00:00:00
abstract::We describe an unexpected feature observed for the heterologous expression of the Thyrinteina arnobia cypovirus polyhedrin from a recombinant baculovirus infection in different insect cell lines. The in cellulo-formed crystals varied in size and shape depending on the cell line. Crystals formed in Trichoplusia ni-deri...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04214-z
更新日期:2019-06-01 00:00:00
abstract::Human diploid BAMB cells with epitheloid morphology, which had been derived from amniotic fluid cells, were capable of supporting the replication of human cytomegalovirus (CMV), without prior treatment of the cells with halogenated pyrimidines. The growth of this virus in BAMB cells and in human diploid fibroblastoid ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315617
更新日期:1976-01-01 00:00:00
abstract::Rabbit convalescent and hyperimmune sera, human patient and blood donor sera, as well as cerebrospinal fluids of humans with herpes simplex virus encephalitis all recognize similar major antigenic components in herpes simplex virus infected rabbit or human cells as shown by electrophoretic analysis of immunoprecipitat...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314855
更新日期:1977-01-01 00:00:00
abstract::A putative novel positive-sense (+) RNA virus was detected in isolate CF16158 of the fungus Fusarium graminearum, the causal agent of Fusarium head blight and crown rot in wheat in China. The full genome of this virus was sequenced and characterized. The complete cDNA sequence is 7,051 nt long and contains four open r...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04486-5
更新日期:2020-02-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0864-0
更新日期:2011-03-01 00:00:00
abstract::A quasi-spherical virus was isolated from a cultivated Amazon lily plant (Eucharis grandiflora) that could be mechanically transmitted to healthy E. grandiflora plants, subsequently producing mild mosaic or mottle symptoms on the leaves. The purified virus consisted of three quasi-spherical particles about 20 nm wide ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1467-8
更新日期:2013-01-01 00:00:00
abstract::Respiratory syncytial virus (RSV) is the most frequently identified viral agent in infants, children, and elderly people with acute respiratory tract infections (ARTIs). This study is the only one of its kind in Kuwait, and its purpose was to investigate the genetic variability of the G protein gene in RSV strains pre...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-3881-z
更新日期:2018-09-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310626
更新日期:1990-01-01 00:00:00
abstract::Amino acids Lys34, His36, and Phe37 were substituted by PCR-mediated, site-directed mutagenesis for three Trp's in the AcNPV polyhedrin sequence. Phase contrast microscopy revealed refringent, amorphous polyhedra in the nuclei of infected cells. Electron microscopy confirmed a great variation in form and size of the m...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050675
更新日期:2000-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317427
更新日期:1975-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01322691
更新日期:1995-01-01 00:00:00
abstract::Two cell lines, CNS-5 and CNS-6, were established by cocultivation of sedimented cells in cerebrospinal fluid (CSF) from two anti-human T-lymphotropic virus type I (HTLV-I) antibody-positive male patients with encephalopathy and HTLV-I-associated myelopathy, respectively, with peripheral blood mononuclear cells from a...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01311306
更新日期:1991-01-01 00:00:00
abstract::Type I interferon (IFN) exerts strong antiviral activity, particularly against human immunodeficiency virus (HIV), and although several viral proteins have been shown to deregulate IFN induction, little is known about the induction of type I IFNs by HIV RNAs. In the present study, we used the stable isotope labeling w...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2372-8
更新日期:2015-05-01 00:00:00
abstract::In this study, we isolated a novel virulent Escherichia phage, SRT7. Its genome is a double-stranded linear DNA molecule containing 39,883 bp. Direct terminal repeats with a length of 175 bp, are present at both ends of the genome. The G+C content is 50.54%. Forty-seven putative protein coding genes were identified. N...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04182-4
更新日期:2019-04-01 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration with high mortality rates in swine. It has become increasingly problematic in China. Since the nucleocapsid (N) protein is highly conserved, it is a candidate protein for early diagnosis and vaccine development. In this study, the N genes of ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1592-4
更新日期:2013-06-01 00:00:00
abstract::The genome of adenovirus (AV) types 1, 2, and 5 is known to be very variable as evidenced by the great number of genome types described (36, 61, and 35 for AV1, 2, and 5 respectively). Physical maps were constructed for nearly all of the restriction or R-variants by biochemical methods and by adapting restriction frag...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718606
更新日期:1996-01-01 00:00:00
abstract::To trace the possible route of introduction of porcine epidemic diarrhea virus (PEDV), the phylogenetic relationships of PEDV strains in the regions of epidemicity in the Philippines to PEDV strains that are endemic in other countries were investigated. Partial nucleotide sequences of the S1 spike gene was determined ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2938-0
更新日期:2016-09-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01320782
更新日期:1982-01-01 00:00:00
abstract::Providence Virus (PrV) is a non-envoloped, T = 4 icosahedral beta-tetravirus that undergoes autocatalytic cleavage of its coat protein precursor after capsid assembly. This is also a well characterized function of Nudaurelia capensis omega virus (NomegaV), a member of the related omegatetraviruses, whose x-ray structu...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-005-0637-3
更新日期:2006-01-01 00:00:00
abstract::Long terminal repeats (LTRs) of human endogenous retroviruses (HERVs) have been reported to serve as alternative promoters in functional genes. The GSDML (gasdermin-like protein) gene located on human chromosome 17q21 has been found to be an oncogenomic recombination hotspot. Here, we identified the LTR element of HER...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-006-0764-5
更新日期:2006-10-01 00:00:00
abstract::Human papilloma virus DNA has been examined for heterogeneity by electron microscopy, reassociation kinetics, and restriction endonuclease digestion. No heterogeneity in contour length was detected. The reassociation rate of papilloma DNA isolated from pooled warts was consistent with that expected for a homogeneous, ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314794
更新日期:1977-01-01 00:00:00
abstract::Genetically engineered expression of replicase encoding sequences has been proposed as an efficient system to confer protection against virus diseases by eliciting protection mechanisms in the plant. Potato leaf-roll was one of the first diseases for which this kind of protection was engineered in potato plants. Howev...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050170095
更新日期:2001-07-01 00:00:00
abstract::Seven-day-old embryonated hen eggs were infected with African Horse Sickness virus by the yolk sac and intravenous routes. Virus reached a high titre in the blood of infected embryos. Culicoides variipennis midges which took a blood meal from infected eggs became infected with virus, and after 7 days at 26 degrees - 2...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01347975
更新日期:1975-01-01 00:00:00
abstract::China experienced an outbreak of equine influenza during 2007-2008. Meanwhile, its neighbor countries, such as Mongolia, India and Japan, have also been affected by various influenza virus strains in each country. Phylogenetic analysis showed that the newly emerging Chinese strains belong to Florida sublineage clade 2...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0724-y
更新日期:2010-09-01 00:00:00
abstract::The molecular epidemiology of a recent norovirus (NoV) outbreak in Brazil performed by comparative analysis with Genebank NoV sequences showed that the GII.4 strain was responsible for 72.5% of all NoV-positive cases (58/80). Other detected NoV strains included GII.3 (7/80; 8.8%) and GII.9 (8/80; 10%). This is the fir...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0078-x
更新日期:2008-01-01 00:00:00