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Detection of anti-gag antibodies of feline immunodeficiency virus in cat sera by enzyme-linked immunosorbent assay.

Abstract：

:Using gag protein of feline immunodeficiency virus (FIV) expressed in Escherichia coli, an enzyme-linked immunosorbent assay (ELISA) system was developed for detection of antibodies to FIV gag protein in cat sera. With serum samples from cats experimentally infected with several strains and an infectious molecular clone of FIV, increases of the antibody titers to FIV gag protein were observed in all cases by the ELISA at early stage of infection. When we examined a total of 415 field cat sera which were previously tested by an indirect immunofluorescence assay (IFA), 9 (12.9%) out of 70 IFA positive sera were judged as negative by the ELISA. However, all 3 serum samples tested among the 9 IFA positive sera had antibodies to gp130 but not to p26 by a radioimmunoprecipitation assay. The results indicated that some IFA positive sera did not have antibodies to the p26 though they have antibodies to other proteins specific for FIV.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Furuya T,Hasegawa A,Miyazawa T,Miki K,Mikami T

doi

10.1007/BF01309815

keywords:

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

355-61

issue

3-4

eissn

0304-8608

issn

1432-8798

journal_volume

124

pub_type

杂志文章

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