Abstract：
:The temperature sensitivity of herpes simplex virus type 1 (HSV-1) was assessed in primary cultures of mouse central nervous system (MNS) cells and mouse embryo cells (MEC). Infectious yields were determined and the ultrastructural morphogenesis of HSV-1 particles was compared following incubation at 37 or 40.5 degrees C. Yields of infectious virus were significantly reduced for both types of cell cultures following incubation at 40.5 degrees C. However, the effect of supraoptimal temperature (40.5 degrees C) on HSV-1 replication in MEC was significantly greater than the effect of supraoptimal temperature on virus replication in MNS cells. With respect to viral morphogenesis, no significant differences were found in either the quantity or the appearance (empty versus electron opaque core) of intranuclear particles present per infected nucleus, regardless of cell type or incubation temperature. However, complete virus particles (enveloped capsids with dense cores) were never observed in MEC at 40.5 degrees C, either intracytoplasmically or extracellularly. In contrast, complete virus particles were observed in MNS cell cultures at 40.5 degrees C, albeit in reduced numbers. At the permissive temperature (37 degrees C), complete intracytoplasmic and/or extracellular virus particles were associated with every infected cell in the MNS cell or MEC cultures. Thus an interactional effect on HSV-1 replication was found between cell culture type and incubation temperature.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Cole NLdoi
10.1007/BF01309574keywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
49-63issue
1-4eissn
0304-8608issn
1432-8798journal_volume
127pub_type
杂志文章abstract：:Rabbit convalescent and hyperimmune sera, human patient and blood donor sera, as well as cerebrospinal fluids of humans with herpes simplex virus encephalitis all recognize similar major antigenic components in herpes simplex virus infected rabbit or human cells as shown by electrophoretic analysis of immunoprecipitat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314855
更新日期：1977-01-01 00:00:00
abstract：:Nanoviruses are multi-component plant-infecting single-stranded DNA viruses. Using a viral metagenomics-informed approach, a new nanovirus and two associated alphasatellite molecules have been identified in an uncultivated asymptomatic Vicia cracca plant in the Rhône region of France. This novel nanovirus genome inclu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3634-4
更新日期：2018-03-01 00:00:00
abstract：:The genomes of two neurovirulent strains of poliovirus type 3, wild type P3/Leon/37 and a vaccine revertant P3/119/70, have been cloned in E. coli. The cDNA: RNA hybrid method used was efficient and may have wide applicability for cDNA cloning. Overlapping clones spanning the entire genome were obtained for each strai...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309297
更新日期：1984-01-01 00:00:00
abstract：:A virus isolated from Olea europaea L. grown in Portugal, was identified as a member of the species Tobacco necrosis virus D (TNV-D, genus Necrovirus, family Tombusviridae), based on the molecular and serological properties of the purified virus particles. The genomic region encoding the coat protein (CP) of this isol...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0258-7
更新日期：2004-06-01 00:00:00
abstract：:The acid-extractable leaf proteins of potato spindle tuber viroid (PSTV) infected tomato plants were analysed electrophoretically on polyacrylamide gels. The most prominent alteration found during disease development was the appearance of a "pathogenesis-related" protein with an apparent molecular weight of 14,000 (ca...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309998
更新日期：1984-01-01 00:00:00
abstract：:We present the molecular characterization of a new virus infecting yerba mate (Ilex paraguariensis St. Hil.) in Argentina. Deep sequencing of diseased yerba mate plants showing chlorotic linear patterns, chlorotic rings, and vein yellowing resulted in the identification of a new virus resembling plant rhabdoviruses in...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3363-8
更新日期：2017-08-01 00:00:00
abstract：:In 2010, yam beans in a field trial in Peru showed viral disease symptoms. Graft-transmission and positive ELISA results using potyvirus-specific antibodies suggested that the symptoms could be the result of a potyviral infection. Small interfering RNA (siRNA) were extracted from one of the samples and sent for high-t...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1214-6
更新日期：2012-04-01 00:00:00
abstract：:To evaluate the variation of Potato mop-top pomovirus from potato fields, 21 isolates were collected from different Danish locations. Reverse transcription-polymerase chain reaction-restriction fragment length polymorphism (RT-PCR-RFLP) of regions of RNA2 was performed for all 21 isolates resulting in the establishmen...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-002-0926-z
更新日期：2003-02-01 00:00:00
abstract：:Cotton leaf curl disease (CLCuD) is a major limitation to cotton production on the Indian subcontinent. A survey for viruses causing CLCuD was conducted during the 2009 and 2010 cropping seasons in the northwestern Indian cotton-growing belt in the states of Punjab, Haryana and Rajasthan. Partial sequences of 258 and ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1225-y
更新日期：2012-05-01 00:00:00
abstract：:The '6K1' protein of the Pinellia isolate of Soybean mosaic virus was cloned into a prokaryotic expression vector and a polyclonal antiserum raised to the expressed fusion protein. In immunogold labeling of thin sections of infected leaves of Pinellia ternata, specific labeling occurred at the cell periphery. This mig...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0972-7
更新日期：2007-01-01 00:00:00
abstract：:A novel bisegmented double-stranded RNA virus was identified in the mycoparasitic and opportunistic fungus Hypomyces chrysospermus. The RNA1 genome segment comprises 1866 bp and encodes an RNA-dependent RNA polymerase (RdRp). The RNA2 segment comprises 1822 bp and encodes a capsid protein. Phylogenetic analysis of the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-019-04359-x
更新日期：2019-10-01 00:00:00
abstract：:Brugmansia suaveolens mottle virus (BsMoV) was the first potyvirus isolated from "angel trumpet" (Brugmansia suaveolens), described in Brazil. In this study, the complete nucleotide (nt) genome sequence of BsMoV was determined, and the deduced amino acid (aa) sequence was analyzed. The BsMoV RNA genome consists of 987...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0798-6
更新日期：2010-10-01 00:00:00
abstract：:We previously generated an RCMV strain in which the r144 gene, encoding a major histocompatibility complex class I homolog, had been deleted (RCMVdelta r144). To investigate the role of r144 during acute infection of neonatal rats, we infected three days-old neonatal rats with either RCMVdelta r144 or wild type (wt) R...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050200028
更新日期：2002-04-01 00:00:00
abstract：:Genomic information about Muscovy duck parvovirus is still limited. In this study, the genome of the pathogenic MDPV strain YY was sequenced. The full-length genome of YY is 5075 nucleotides (nt) long, 57 nt shorter than that of strain FM. Sequence alignment indicates that the 5' and 3' inverted terminal repeats (ITR)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2946-0
更新日期：2016-09-01 00:00:00
abstract：:We report the complete genome sequencing of two Escherichia coli T5-related bacteriophages, DT57C and DT571/2, isolated from the same specimen of horse feces. These two isolates share 96% nucleotide sequence identity and can thus be considered representatives of the same novel species within the genus T5likevirus. The...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2582-0
更新日期：2015-12-01 00:00:00
abstract：:RNA 3 of alfalfa mosaic virus (AMV) encodes the 5'-proximal movement protein (MP) gene and the 3'-proximal coat protein (CP) gene which is expressed from a subgenomic RNA. Several strategies were explored to use this RNA as a vector for expression of the green fluorescent protein (GFP) in Nicotiana tabaccum plants exp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170125
更新日期：2001-01-01 00:00:00
abstract：:The gene coding for the nucleocapsid protein NP of the influenza A virus recombinant strain 413 1,1 was characterized biochemically by molecular hybridization and fingerprint analysis. The data presented suggest that this NP gene has evolved by intracistronic recombination between NP genes of virus N and the fowl plag...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322701
更新日期：1980-01-01 00:00:00
abstract：:Bovine lactoferrin (LF) and ribavirin (Rbv) were tested as antiviral agents against Seoul type hantavirus (SR-11 strain) in vitro. Hantaviral foci number in Vero E6 cells infected with SR-11 was reduced with LF treatment by 5 days post infection to obtain a 50% effective dose (ED50) of 2500 microg/ml, while pretreatme...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070077
更新日期：2000-01-01 00:00:00
abstract：:Transmission electron microscopy was used to confirm the presence of picorna-like virus particles presumed to be Homalodisca coagulata virus-1 (HoCV-1) in the midgut region of adult glassy-winged sharpshooters (GWSS). In addition, we offer a reverse transcription polymerase chain reaction (RT-PCR) assay for the detect...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-1066-2
更新日期：2008-01-01 00:00:00
abstract：:A one-year influenza A virus (IAV) monitoring program was conducted in a live-bird market (LBM) in Thailand. Using one-step real-time RT-PCR (rRT-PCR), 2.39 % of live birds were found to be IAV positive. Twenty viruses could be identified as IAV subtype H7N6. Eight IAV-H7N6 viruses were subjected to whole-genome seque...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2759-1
更新日期：2016-05-01 00:00:00
abstract：:Results demonstrating involvement of glycoprotein C (gC) of herpes simplex type 1 virus (HSV-1) in attachment of the virus to the cell are presented. Monoclonal antibodies against gC-1 inhibited adsorption of gC(+)-strains. The gC(-)-mutant, MP, attached to cells but at a reduced rate. Attachment of the MP-mutant was ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310482
更新日期：1991-01-01 00:00:00
abstract：:Manipulation of African swine fever virus (ASFV) genomes, in particular those from field strains, is still a challenge. We have shown recently that generation of a green-fluorescent-protein-expressing, thymidine-kinase-negative (TK-) mutant of the low-pathogenic African swine fever virus field strain NHV was supported...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2095-2
更新日期：2014-09-01 00:00:00
abstract：:We investigated the frequency and the clinical relevance of hepatitis B virus (HBV) pre-S mutations in Qidong, China. The results showed HBV pre-S mutants were detected in 48.4% (47/97) of patients with HBV infection. Both pre-S deletion and pre-S2 start codon mutations were more frequently found in HCC than in CH pat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0176-9
更新日期：2008-01-01 00:00:00
abstract：:The polypeptides associated with fowlpox virus (FPV) infection of chicken embryo skin (CES) cells were examined by metabolic labelling with [35S]-methionine and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Polypeptide synthesis was followed over the first 48 hours post infection, as this was s...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320959
更新日期：1987-01-01 00:00:00
abstract：:Multiple splicing sites exist in the RNA genome of the human immunodeficiency virus type 1 (HIV-1). In a screen for subgenomic forms of the HIV-1 genome that could be transferred to fresh cells by virus infection, we identified a novel spliced variant of HIV-1 RNA that uses a hitherto unknown splice acceptor site with...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718159
更新日期：1996-01-01 00:00:00
abstract：:Papillomavirus capsid proteins L1 and L2 mediate virion attachment, internalization and trafficking. In our studies of the capsid proteins, we identified an interaction of L2 with the E3 ligase Smad ubiquitin regulatory factor 2 (Smurf2). Smurf2 expression alters BPV1 virion trafficking and L2 protein levels. Using BP...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-0924-0
更新日期：2011-05-01 00:00:00
abstract：:Twenty TGEV isolates were compared by sequencing a 393-414 nucleotide stretch near the 5' end of the S gene, after amplification by RT-PCR. This part of the S gene is known to show considerable variation between porcine, canine and feline coronaviruses and is completely deleted from porcine respiratory coronaviruses. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050191
更新日期：1997-01-01 00:00:00
abstract：:The DNA of mammalian cells treated with 4,5',8-trimethylpsoralen (Trioxsalen) is crosslinked (preventing transcription) only after photoactivation with near ultraviolet light. Suppression of gene action in cocultivated or fused cultures, including both induction and antiviral action of fibroblast interferon, can conse...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320762
更新日期：1980-01-01 00:00:00
abstract：:During the epidemic outbreak in the region of Greifswald in the winter 1974/75, we found influenza virus variants which showed differences in the electrophoretic mobility of HA. Among the 25 isolates 13 were of slower and 12 of higher mobility. HA1 of 6 isolates was studied by determining the number of the carbohydrat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310484
更新日期：1991-01-01 00:00:00
abstract：:The pathogenicity of 5 different encephalomyocarditis (EMC) virus isolates was investigated in swine fetuses following injection of each virus in utero. Laparotomies were performed on 3 pregnant sows in early mid-third (39-40 days) of gestation and on 5 sows in the late mid-third (70-72 days) of gestation, and groups ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310517
更新日期：1989-01-01 00:00:00
