Abstract：
:Two herpes simplex type 1 (HSV-1) recombinant viruses carrying the hepatitis C virus (HCV) NS5A open reading frame under the control of the cytomegalovirus immediate early (IE) or a herpes simplex chimeric promoter (alpha 4 gamma 1UL19) were constructed and characterized. Expression studies showed that both HSV-NS5A recombinant viruses were able to express high levels of the NS5A protein in infected cells. Most importantly, using this system, we demonstrated that the NS5A protein interacts with the growth receptor-bound protein 2 (Grb2) and inhibits the phosphorylation of the extracellular signal-regulated kinases 1 and 2 (ERK1/2) in HeLa, NIH3T3 or liver infected cells. Thus, our studies confirm the ability of the NS5A protein to perturb the extracellular signal-regulated kinase (ERK) pathway in HeLa cells by the use of an alternative system for NS5A expression and extend this observation to additional cell lines. We conclude that HSV-based viral vectors may provide a useful system for studying the expression and selected functional properties of the HCV NS5A protein.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Georgopoulou U,Caravokiri K,Mavromara Pdoi
10.1007/s00705-002-0925-0keywords:
subject
Has Abstractpub_date
2003-02-01 00:00:00pages
237-51issue
2eissn
0304-8608issn
1432-8798journal_volume
148pub_type
杂志文章abstract：:Phages, viruses targeting bacteria, have potential therapeutic applications in the control of infections with antibiotic-resistant bacteria. In this study, an Enterobacter cloacae phage, Ec_L1, was isolated from sewage sludge samples collected from a hospital. The genome of phage Ec_L1 consists of 51,894 bp with 48.24...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04672-w
更新日期：2020-08-01 00:00:00
abstract：:Tax oncoprotein of Human T-lymphotropic virus 1 (HTLV-1) has been proposed to dysregulate the expression of a number of cellular genes, many of which play a critical role for cell proliferation. Our initial data demonstrated that the immediate-early gene butyrate response factor 1 ( BRF1) was upregulated in HTLV-1-inf...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0114-9
更新日期：2003-09-01 00:00:00
abstract：:Avian influenza virus infections are a major cause of morbidity and rapid identification of the virus has important clinical, economical and epidemiological implications. We have developed a one-tube Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) for the rapid diagnosis of avian influenza A. A panel of refer...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170193
更新日期：2001-01-01 00:00:00
abstract：:The bovine papillomavirus (BPV) transforming gene E5 is thought to modulate growth factor receptor function leading to a stimulation of growth factor signal transduction pathways. However, the influence of E5 on the range of receptor mediated changes in protein phosphorylation has not been addressed. We looked for the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050131
更新日期：1997-01-01 00:00:00
abstract：:The use of the complete DNA sequence for the Marek's disease virus (MDV) serotype 1 vaccine strain CVI988 Rispens in comparative genomic studies with virulent strains of MDV has revealed the presence of a number of insertions, deletions and single-nucleotide polymorphisms. In this study, we investigated a SNP in the H...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-008-0152-4
更新日期：2008-01-01 00:00:00
abstract：:Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). We used microchip electrophoresis in combination with automatic image analysis to develop a novel high-throughput PCR-RFLP to type the gene sequences that encode BLV Tax 233. This method revealed that 233L-Tax is more prevalent than ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04842-w
更新日期：2020-12-01 00:00:00
abstract：:Since the first rescue of a recombinant Newcastle disease virus (rNDV) in the late 1990s, many more rNDVs have been rescued by researchers around the world. Regardless of methodology, the main principle behind rescue of the virus has remained the same, i.e., the formation of a functional replication complex by simulta...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s00705-016-3065-7
更新日期：2017-01-01 00:00:00
abstract：:We previously generated an RCMV strain in which the r144 gene, encoding a major histocompatibility complex class I homolog, had been deleted (RCMVdelta r144). To investigate the role of r144 during acute infection of neonatal rats, we infected three days-old neonatal rats with either RCMVdelta r144 or wild type (wt) R...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050200028
更新日期：2002-04-01 00:00:00
abstract：:Genetic relationships between the genome segments of six cypovirus (CPV) isolates were analysed by RNA cross-hybridisation. These included three type 1 viruses and single isolates of types 2, 5 and 12, which collectively are identical to those previously compared by serology and electrophoresis [Mertens et al. (1989),...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050525
更新日期：1999-01-01 00:00:00
abstract：:Using random amplification and high-throughput sequencing technology a novel picornavirus with dicistronic genome organization and genetically related to canine picodicistrovirus (genus Dicipivirus, family Picornaviridae) was identified and characterized in Northern white-breasted hedgehogs. Hedgehog dicipivirus (hedg...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3565-0
更新日期：2018-01-01 00:00:00
abstract：:A flexuous virus was isolated from cultivated Phaius flavus Lindl. plants in Japan with a latent infection. The virus was assigned to the genus Potexvirus based on morphology and analysis of its complete nucleotide sequence. The genome is 5,816 nucleotides in length, excluding the 3'-terminal poly (A) tail, and contai...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-007-0010-9
更新日期：2008-01-01 00:00:00
abstract：:Antibody and cell-mediated immune responses were measured in rabbits immunized with live, UV inactivated herpes simplex virus or with a subunit vaccine containing envelope proteins. All the types of immunization procedures induced the production of antibody as well as a specific cellular immunity. Furthermore, the sub...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317862
更新日期：1976-01-01 00:00:00
abstract：:The genus Bromovirus currently contains six species whose members have relatively narrow host ranges. In the present work, a new bromovirus infecting elderberry (Sambucus nigra L.) is reported. dsRNA was purified and sequenced by next-generation sequencing, and with minimal additional completion by Sanger sequencing, ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3629-1
更新日期：2018-02-01 00:00:00
abstract：:Two new isolates of cricket paralysis virus, TAR and SIM, are described that were originally isolated from laboratory colonies of Drosophila melanogaster and Drosophila simulans respectively. Using a combination of biological, serological and molecular characters it was possible to distinguish the SIM isolate from all...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718251
更新日期：1996-01-01 00:00:00
abstract：:Sugarcane streak mosaic virus (SCSMV), an economically important causal agent of mosaic disease of sugarcane, is a member of the newly created genus Poacevirus in the family Potyviridae. In this study, we report the molecular characterization of three new SCSMV isolates from China (YN-YZ211 and HN-YZ49) and Myanmar (M...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-2810-2
更新日期：2016-06-01 00:00:00
abstract：:Viruses in the genus Babuvirus have multi-component ssDNA genomes and often associate with alphasatellite molecules containing two common motifs, a common-region stem-loop (CR-SL) involved in initiation of rolling-circle replication and a common-region major (CR-M) motif involved in secondary-strand synthesis. We comp...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3168-1
更新日期：2017-03-01 00:00:00
abstract：:Insertion of foreign DNA containing the E. coli gpt marker by homologous recombination in the pigeonpox virus (PPV) thymidine kinase (TK) gene and selection for the presence of this DNA in the viral genome produced unstable recombinants after 3 plaque purifications. We highlight the persistence of duplicated TK DNA se...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01378643
更新日期：1993-01-01 00:00:00
abstract：:The nucleotide sequence of the 3' terminal region of the Australian isolate of papaya ringspot virus type W [PRSV-W (Aust)] was determined. An open reading frame (864 bp), encoding the putative coat protein gene, occurs upstream of the putative 3' untranslated region (206 bp) and poly(A) tail. A 23 amino acid sequence...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01317141
更新日期：1992-01-01 00:00:00
abstract：:We have investigated whether poly(I:C) Toll-like receptor 3 (TLR3) and resiquimod Toll-like receptor 7 (TLR7) agonists can serve as vaccine adjuvants and promote the efficiency of therapeutic DNA vaccination against tumors expressing the human papilloma virus 16 (HPV-16) E7 protein. For this purpose, C57BL/6 mice were...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-014-2024-4
更新日期：2014-08-01 00:00:00
abstract：:A polymerase chain reaction (PCR) based assay capable of detecting and differentiating seven strains of equine arteritis virus (EAV) from around the world was developed. The primers for the PCR were chosen from the ORF6 gene encoding the unglycosylated membrane protein (M). Viral RNA from cell culture fluids infected ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01322675
更新日期：1995-01-01 00:00:00
abstract：:Two double-stranded RNA (dsRNA) mycoviruses were found in isolate QSP5 of the rice blast fungus Magnaporthe oryzae. Sequence analysis of the two dsRNA mycoviruses revealed that one is closely related to Magnaporthe oryzae virus 2 (MoV2), and the other one is related to Magnaporthe oryzae chrysovirus 1-A (MoCV1-A). The...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2562-4
更新日期：2015-11-01 00:00:00
abstract：:Human papillomaviruses (HPVs) are responsible for the most common human sexually transmitted viral infections. Infection with high-risk HPVs, particularly HPV16, is associated with the development of cervical cancer. The papillomavirus L1 major capsid protein, the basis of the currently marketed vaccines, self-assembl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0484-8
更新日期：2009-01-01 00:00:00
abstract：:Infectious cDNA clones originally derived from a mild strain of Pepper mild mottle virus were constructed by replacing residue 649, a critical point for attenuation of this virus, with all possible amino acids. All clones were infectious to pepper plants and induced a variety of symptoms, including no visible symptoms...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0312-1
更新日期：2009-01-01 00:00:00
abstract：:Human cytomegalovirus disappeared from cultures continuously exposed to interferon. When interferon was removed, high titers of virus reappeared. Cytomegalovirus was thus able to persist in potentially infectious form in cells protected by interferon. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309276
更新日期：1983-01-01 00:00:00
abstract：:Mycobacteriophage archival stocks have been kept for ca. 20-50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreov...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3788-8
更新日期：2018-07-01 00:00:00
abstract：:To evaluate the impact of new antiretroviral combinations (HAART: Highly Active Anti Retroviral Therapy) on HCV replication and liver enzyme levels, we analysed the changes in HCV viremia and aminotransferase levels in HIV and HCV co-infected patients. Moreover, to evaluate the influence of HCV infection on the effica...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050685
更新日期：2000-01-01 00:00:00
abstract：:Two contigs with high similarity to partitivirus sequences were identified by de novo assembly of sequences obtained by RNA-Seq from a wild brassicaceous plant, Arabidopsis halleri subsp. gemmifera. Here, we report the complete genome sequence of a putative novel partitivirus. Excluding the poly-A tail, it consists of...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04670-y
更新日期：2020-09-01 00:00:00
abstract：:Long terminal repeats (LTRs) of human endogenous retroviruses (HERVs) have been reported to serve as alternative promoters in functional genes. The GSDML (gasdermin-like protein) gene located on human chromosome 17q21 has been found to be an oncogenomic recombination hotspot. Here, we identified the LTR element of HER...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0764-5
更新日期：2006-10-01 00:00:00
abstract：:Seven-day-old embryonated hen eggs were infected with African Horse Sickness virus by the yolk sac and intravenous routes. Virus reached a high titre in the blood of infected embryos. Culicoides variipennis midges which took a blood meal from infected eggs became infected with virus, and after 7 days at 26 degrees - 2...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01347975
更新日期：1975-01-01 00:00:00
abstract：:We investigated the in situ localization of the 50 kDa protein encoded by ORF2 of Apple chlorotic leaf spot virus (ACLSV) genome which is thought to be a movement protein. In immunogold electron microscopy of ACLSV-infected Chenopodium quinoa leaves, the 50 kDa protein was localized on plasmodesmata and nearby cytopla...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050660
更新日期：1999-01-01 00:00:00
