Role of the TK+ phenotype in the stability of pigeonpox virus recombinant.

Abstract:

:Insertion of foreign DNA containing the E. coli gpt marker by homologous recombination in the pigeonpox virus (PPV) thymidine kinase (TK) gene and selection for the presence of this DNA in the viral genome produced unstable recombinants after 3 plaque purifications. We highlight the persistence of duplicated TK DNA sequences arising from single crossing over, due to the growth advantage of TK+ virus. Restoration of the TK function by coinsertion of the vaccinia virus TK gene led to stable TK+ recombinants arising from double crossing over.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Letellier C

doi

10.1007/BF01378643

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

431-9

issue

3-4

eissn

0304-8608

issn

1432-8798

journal_volume

131

pub_type

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