Abstract：
:We have investigated whether poly(I:C) Toll-like receptor 3 (TLR3) and resiquimod Toll-like receptor 7 (TLR7) agonists can serve as vaccine adjuvants and promote the efficiency of therapeutic DNA vaccination against tumors expressing the human papilloma virus 16 (HPV-16) E7 protein. For this purpose, C57BL/6 mice were inoculated with 2 × 10(5) TC-1 cells, and they were then immunized with HPV-16 E7 DNA vaccine alone or with 50 μg of resiquimod or poly(I:C) individually. We found that poly(I:C) and resiquimod could induce more antigen-specific lymphocyte proliferation and cytolytic activity compared to vaccination with E7 DNA alone. While E7 DNA had no significant inhibitory effect on tumor growth, co-administration of poly(I:C) and resiquimod with E7 DNA induced significant tumor regression. Peripheral and local cytokine assays demonstrated that co-administration of poly(I:C) and resiquimod with E7 DNA induced circulating antigen-specific IFN-γ and nonspecific intratumoral IL-12. TLR3 and TLR7 agonists can be used to enhance the immune response to DNA vaccine immunogens. Taken together, these data indicate that combined vaccination with DNA encoding HPV-16 E7 plus TLR agonists provides a strategy for improving the efficacy of a vaccine as a possible immunotherapeutic strategy for cervical cancer.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Sajadian A,Tabarraei A,Soleimanjahi H,Fotouhi F,Gorji A,Ghaemi Adoi
10.1007/s00705-014-2024-4subject
Has Abstractpub_date
2014-08-01 00:00:00pages
1951-60issue
8eissn
0304-8608issn
1432-8798journal_volume
159pub_type
杂志文章abstract：:Drosophila C virus RNA acted as mRNA in rabbit reticulocyte lysates and directed the synthesis of at least one capsid protein and a number of higher molecular weight proteins. Kinetic analysis by pulse-chase experiments showed that a number of high molecular weight products acted as precursors to the capsid protein(s)...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311694
更新日期：1983-01-01 00:00:00
abstract：:To understand the mode of persistent infection of Seoul virus in rodents, we examined the distribution of the virus genome and antibody production in infected rats. When 1-day-old rats were inoculated with the KI-83-262 strain, the S segment of viral genome was detected in sera, clots, lungs and kidneys from 3 to 184 ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718634
更新日期：1996-01-01 00:00:00
abstract：:Equine herpesvirus 1 glycoprotein D (EHV-1 gD) expressed constitutively in mammalian cell lines had similar electrophoretic mobility to gD produced in EHV-1 infected cells but lacked a possibly complexed higher molecular weight form seen in the latter. Recombinant gD was N-terminally cleaved at the same site as gD in ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718301
更新日期：1996-01-01 00:00:00
abstract：:A new member of the genus Nepovirus was isolated from blueberry in Japan. The virus was associated with latent infection of blueberry trees and provisionally named blueberry latent spherical virus (BLSV). BLSV was found to have isometric particles approximately 30 nm in diameter, which were composed of a single coat p...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-011-1177-7
更新日期：2012-02-01 00:00:00
abstract：:A cross-sectional study was undertaken during 2012-2013 to determine the prevalence, strains and factors associated with rotavirus infection among under-5-year-old children hospitalized with acute diarrhea in Uganda. Rotaviruses were detected in 37 % (263/712) of the children. The most prevalent strains were G9P[8] (2...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2742-2
更新日期：2016-04-01 00:00:00
abstract：:We have prepared monoclonal antibodies to each of the enteric adenoviruses types 40 and 41. Three different hybridoma cell lines were selected which produced antibody found to react by radioimmunoprecipitation with adenovirus (Ad) hexon antigens. One was specific for Ad 40, another for Ad 41, and a third one reacted w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310718
更新日期：1987-01-01 00:00:00
abstract：:Brefeldin A (BFA) induced a rapid redistribution of vesicular stomatitis virus G protein (VSV-G) to the endoplasmatic reticulum (ER) in interferon (IFN)-pretreated cells. This result is consistent with accumulation of VSV-G in the trans-Golgi (GC) complex in cells pretreated with IFN and implies that IFN does not inte...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314635
更新日期：1992-01-01 00:00:00
abstract：:The nonstructural rotavirus protein NSP1 binds specifically to viral mRNAs and to interferon regulatory factor 3 (IRF3), inducing IRF3 degradation through a proteasome-dependent pathway. By using a vaccinia virus expression system in mammalian cells, we found that the yield of NSP1 was 8- and 13-fold lower than the vi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0850-8
更新日期：2007-02-01 00:00:00
abstract：:The polypeptides associated with fowlpox virus (FPV) infection of chicken embryo skin (CES) cells were examined by metabolic labelling with [35S]-methionine and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Polypeptide synthesis was followed over the first 48 hours post infection, as this was s...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01320959
更新日期：1987-01-01 00:00:00
abstract：:Human bocavirus (HBoV)-2, a new parvovirus, has been identified in stool samples and is suggested to be one of the etiologic agents of acute gastroenteritis (GE). The purpose of this study was to investigate the prevalence of HBoV-2 in children with GE. Stool samples were collected from 358 children hospitalized with ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0533-3
更新日期：2009-01-01 00:00:00
abstract：:Wild plants of the family Convolvulaceae are hosts for a few New World begomoviruses (genus Begomovirus, family Geminiviridae). In this work, we report the complete genome sequence of a new begomovirus infecting the wild convolvulaceous plant Jacquemontia tamnifolia in Venezuela. The cloned bipartite genome showed the...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3372-7
更新日期：2017-08-01 00:00:00
abstract：:Rabies virus (RABV) isolates from two species of canids and three species of bats were analyzed by comparing the C-terminal region of the G gene and the G-L intergenic region of the virus genome. Intercluster identities for the genetic sequences of the isolates showed both regions to be poorly conserved. Phylogenetic ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0671-7
更新日期：2010-06-01 00:00:00
abstract：:TT virus (TTV), the first human circovirus to be discovered, appears to be present in most people; less is known about the prevalence of the related TTV-like mini virus (TLMV). A sensitive nested PCR, specific for TLMV, detected the virus in 48% of 201 sera (Norwegian blood donors) previously found to have a 90% preva...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s705-002-8310-6
更新日期：2002-01-01 00:00:00
abstract：:Infection of soybean by the plant cytorhabdovirus soybean blotchy mosaic virus (SbBMV) results in significant yield losses in the temperate, lower-lying soybean production regions of South Africa. A 277 bp portion of the RNA-dependent RNA polymerase gene of 66 SbBMV isolates from different: hosts, geographical locatio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3722-0
更新日期：2018-05-01 00:00:00
abstract：:Viral infection is usually initiated by the binding of virus particles to specific receptor molecule(s) on the host cell surface. Blocking of this step prevents the following step, penetration into the cell. In the present study, we investigated the virus-cell interactions of virions of Norwalk virus (NV), a major eti...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0143-4
更新日期：2003-09-01 00:00:00
abstract：:Shorebirds on their southerly migration from Siberia to Australia, may pass through Asian regions currently experiencing outbreaks of highly pathogenic H5N1 influenza. To test for the presence of avian influenza viruses in migratory shorebirds arriving in Australia during spring 2004, 173 cloacal swabs were collected ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0784-1
更新日期：2006-11-01 00:00:00
abstract：:Porcine circovirus type 2 (PCV2) is the etiological agent associated with several pig diseases that are collectively referred to as porcine circovirus-associated disease (PCVAD). Unfortunately, PCV2 has had a serious economic impact on the swine industry. In this study, we report the genome sequence of a novel PCV2 is...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2615-8
更新日期：2015-12-01 00:00:00
abstract：:To eradicate rabies in foxes, almost 97 million oral rabies vaccine baits have been distributed in Germany and Austria since 1983 and 1986, respectively. Since 2007, no terrestrial cases have been reported in either country. The most widely used oral rabies vaccine viruses in these countries were SAD (Street Alabama D...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0408-7
更新日期：2009-01-01 00:00:00
abstract：:Ninety four percent of the genome of bovine herpesvirus 4 (BHV-4) strain DN 599 was cloned and a physical map was constructed by Southern blot analysis using a library of cloned fragments cleaved with the 3 restriction enzymes (Eco RI, Bam HI, and Hin dIII). The genome length was estimated to be 156.5 kbp +/- 0.7. The...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309576
更新日期：1992-01-01 00:00:00
abstract：:The movement protein (MP) of tobacco mosaic virus (TMV) mediates the transport of viral RNA from infected cells to neighboring uninfected cells via plasmodesmata by interacting with putative host factors. To find such host factors, we screened tobacco proteins using the yeast two-hybrid system. NtMPIP1, a novel subset...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0397-6
更新日期：2009-01-01 00:00:00
abstract：:Hepatitis C virus (HCV) genome contains a 3'noncoding region (3'NCR) consisting of a variable region, a polypyrimidine tract (polyU/UC) and the X region. To examine the roles of 3'NCR and polyU/UC tract in the internal ribosome entry site (IRES)-mediated translation process, a variety of 3'NCRs containing different le...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-005-0491-3
更新日期：2005-06-01 00:00:00
abstract：:In vivo transfection by intramuscular injection with plasmids expressing the immunogenic proteins of microbial pathogens has considerable potential as a vaccination strategy against many pathogens of both man and animals. Here we report that weanling mice given a single intramuscular injection of 50 micrograms of a pl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718332
更新日期：1996-01-01 00:00:00
abstract：:Sendai virus given intravenously into CFLP mice produced dose-related suppression of the acute inflammation produced by carrageenan and 5-HT. The inhibition correlated with interferon levels in the blood, suggesting that interferon may account for the virus-induced anti-inflammatory effect. ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314606
更新日期：1981-01-01 00:00:00
abstract：:Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major anti...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070087
更新日期：2000-01-01 00:00:00
abstract：:Adenoviruses are common pathogens that are responsible for a wide variety of infectious syndromes. The objectives of this study were to identify and characterize members of different adenovirus species at the molecular level and to describe the correlation between viruses and clinical syndromes during a period of 4 ye...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0338-4
更新日期：2009-01-01 00:00:00
abstract：:The sequence variability of Barley yellow dwarf virus-PAV (PAV) and Barley yellow dwarf virus-MAV (MAV) was studied by comparing 502 nucleotides from the coat protein-encoding region of six isolates from Latvia and four from Sweden. The diversity within MAV was low (>97% sequence identity), also when compared to isola...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0242-2
更新日期：2004-04-01 00:00:00
abstract：:In this study, we describe a novel mycovirus isolated from Trichoderma asperellum, which was designated Trichoderma asperellum dsRNA Virus 1 (TaRV1). The sequence analysis revealed that TaRV1 has two discontinuous open reading frames (ORF), ORF1 and ORF2. A hypothetical protein and an RNA-dependent RNA polymerase are ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3988-2
更新日期：2018-12-01 00:00:00
abstract：:Using monoclonal antibodies raised against a Faba bean necrotic yellows virus (FBNYV) isolate from Egypt and a Faba bean necrotic stunt virus (FBNSV) isolate from Ethiopia, a striking serological variability among nanovirus isolates from faba bean in Morocco was revealed. To obtain a better understanding of this nanov...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0548-9
更新日期：2010-01-01 00:00:00
abstract：:Noninfectious virus particles were produced in Ehrlich ascites tumor cells infected intraperitoneally with fowl plague virus. The PFU yield of virus per cell was less than 0.1 and the ratio PFU/HA units in the progeny virus was less than 10(3). The virus particles had the same morphology and size as egg-grown virus bu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315278
更新日期：1978-01-01 00:00:00
abstract：:Long terminal repeats (LTRs) of human endogenous retroviruses (HERVs) have been reported to serve as alternative promoters in functional genes. The GSDML (gasdermin-like protein) gene located on human chromosome 17q21 has been found to be an oncogenomic recombination hotspot. Here, we identified the LTR element of HER...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0764-5
更新日期：2006-10-01 00:00:00
