Abstract:
:Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major antigenic sites of the protein. Extracts from potato tubers expressing N-gS were inoculated intraperitoneally to mice, and the vaccinated mice developed serum IgG specific for TGEV. Furthermore, when potato tubers expressing N-gS were fed directly to mice, they developed serum antibodies specific for gS protein, demonstrating the oral immunogenicity of the plant derived spike protein from TGEV.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Gómez N,Wigdorovitz A,Castañón S,Gil F,Ordás R,Borca MV,Escribano JMdoi
10.1007/s007050070087keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
1725-32issue
8eissn
0304-8608issn
1432-8798journal_volume
145pub_type
杂志文章abstract::One host polypeptide (40,000 daltons) synthesized prior to infection is associated with the 250S RNA polymerase structure partially purified by a combination of velocity sedimentation and isopycnic separation. A series of pulse-chase experiments have shown that a 56,000 dalton polypeptide made during the eclipse phase...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317808
更新日期:1975-01-01 00:00:00
abstract::The effect of the local anaesthetic drug procaine on the adsorption of two enveloped viruses was studied. Physiological concentrations of the drug (7 X 10(-3)--7 X 10(-2) M) strongly inhibited the adsorption of both West-Nile and herpes viruses as determined by plaque assay and the infective center assay. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317291
更新日期:1978-01-01 00:00:00
abstract::Influenza virus A/WSN/33 forms large plaques (> 3 mm diameter) on MDCK cells whereas A/Aichi/2/68 forms only small plaques (< 1 mm diameter). Fast growing reassortants (AWM), isolated by mixed infection of MDCK cells with these two virus strains in the presence of anti-WSN antibodies, all carried the M gene from WSN. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01313769
更新日期:1993-01-01 00:00:00
abstract::A novel bisegmented double-stranded RNA virus was identified in the mycoparasitic and opportunistic fungus Hypomyces chrysospermus. The RNA1 genome segment comprises 1866 bp and encodes an RNA-dependent RNA polymerase (RdRp). The RNA2 segment comprises 1822 bp and encodes a capsid protein. Phylogenetic analysis of the...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04359-x
更新日期:2019-10-01 00:00:00
abstract::Here we determined the relative expression of HERV-K and W proviruses in HIV infected and non-infected mothers as well as their respective babies up to 1 year-old. HIV-infected mothers, their babies and uninfected control groups presented expression of both HERV-K and HERV-W with relatively high frequency. While the l...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3167-2
更新日期:2017-03-01 00:00:00
abstract::Classic neutralization studies by Fazekas de St. Groth and Webster (8) on mixtures of influenza viruses and mixtures of rabbit antisera are reinterpreted in terms of a percentage contaminant in the stock used for the dilution series. A very small amount of a different virus changes the shape of quantal assay curves co...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317497
更新日期:1979-01-01 00:00:00
abstract::The structure and organization of the large (L) RNA of iris yellow spot virus (IYSV) was determined, and with this report, the complete genomic sequence of IYSV of the genus Tospovirus, family Bunyaviridae has been elucidated. The L RNA of IYSV was 8,880 nucleotides in length and contained a single open reading frame ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0568-5
更新日期:2010-02-01 00:00:00
abstract::Changes in the NP gene of H5N1 highly pathogenic avian influenza (HPAI) viruses have previously been shown to affect viral replication, alter host gene expression levels and affect mean death times in infected chickens. Five amino acids at positions 22, 184, 400, 406, and 423 were different between the two recombinant...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0399-4
更新日期:2009-01-01 00:00:00
abstract::The highly conserved encapsidation signal (epsilon) of hepatitis B viral (HBV) pregenomic RNA has been reported as an essential component for encapsidation and protein priming of HBV polymerase. Here, we report that two HBV epsilon RNA-binding host proteins (80 and 43 kDa) and a copurifying protein (100 kDa) were puri...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050200001
更新日期:2002-03-01 00:00:00
abstract::A total of 72 Borna Disease virus infected rabbits were treated with different concentrations of cyclophosphamide, glucocorticoids or both in combination. Comparison with untreated, infected rabbits showed a drastic alteration in the clinical picture, a considerable prolongation of the survival time, and differences i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318132
更新日期:1981-01-01 00:00:00
abstract::The CPK cells derived from swine kidney were infected with the attenuated TO-163 strain of transmissible gastroenteritis (TGE) virus, and fused with uninfected Vero cells in the presence of polyethylene glycol. Repeated cocultivation of the fused cells with uninfected Vero cells rendered the virus to grow in Vero cell...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01321128
更新日期:1992-01-01 00:00:00
abstract::Amino acid positions recognized by monoclonal antibodies (MAbs) in the influenza A virus nucleoprotein (NP) have been reported. As these residues were scattered in the three-dimensional (3D) structure of NP, no patterns of the architecture of antibody-binding sites could be inferred. Here, we used site-specific mutage...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1952-8
更新日期:2014-06-01 00:00:00
abstract::Non-neoplastic tonsils were analyzed for detection of Epstein-Barr virus (EBV)-positive cells by in situ hybridization and in situ PCR. EBV-encoded small nuclear RNA 1(EBER1)-positive cells were found in 28.2% of the tonsils and were evenly localized in the extrafollicular area and within germinal centers. Latent memb...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050332
更新日期:1998-01-01 00:00:00
abstract::The complete nucleotide sequence of genome segment S9, which was reported as the ninth largest among the 12 genome segments of rice dwarf virus, was determined. The segment was 1,305 bp long and coded for a protein composed of 351 amino acids (Mr 38,930). The terminal sequences of the segment were found to consist of ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01313885
更新日期:1989-01-01 00:00:00
abstract::The genomic sequence of an El Amar isolate of plum pox virus (PPV) from Egypt was determined by sequencing overlapping cDNA fragments. This is the first complete sequence of a member of the El Amar (EA) strain of PPV. The genome consists of 9791 nt, excluding a poly(A) tail at the 3' terminus. The complete nt sequence...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-005-0703-x
更新日期:2006-06-01 00:00:00
abstract::Optimal conditions of radial haemolysis in gel (HIG) with Chikungunya (Chik) and Japanese encephalitis (JE) viruses were developed and compared with those of rubella virus. Sheep erythrocytes were found preferable for HIG with Chik and JE viruses, unlike rubella virus, where goose erythrocytes gave best results. Roost...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01320619
更新日期:1980-01-01 00:00:00
abstract::The complete nucleotide sequence of Subterranean clover mottle virus (SCMoV) genomic RNA has been determined. The SCMoV genome is 4,258 nucleotides in length. It shares most nucleotide and amino acid sequence identity with the genome of Lucerne transient streak virus (LTSV). SCMoV RNA encodes four overlapping open rea...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0144-3
更新日期:2003-11-01 00:00:00
abstract::We previously reported the isolation of South River virus (SORV) from a pool of mosquitoes collected in the Yucatan Peninsula of Mexico (Farfan-Ale et al. in Vector Borne Zoonotic Dis 10:777-783, 5). The isolate (designated SORV-252) was identified as SORV after a 197-nucleotide region of its small RNA genome segment ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1280-4
更新日期:2012-06-01 00:00:00
abstract::Since late 2018, foot-and-mouth disease (FMD) has reemerged and rapidly swept through pig farms in North and Central Vietnam, despite widespread use of commercial FMD vaccines. To investigate the FMD virus (FMDV) strains responsible for the current epidemics, 40 FMDV samples were collected from 17 provinces during Nov...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04763-8
更新日期:2020-11-01 00:00:00
abstract::During the surveillance for avian influenza viruses (AIVs) in live poultry markets in Eastern China, in 2016, a novel reassortant H7N6 AIV was isolated from a chicken. Phylogenetic analysis showed that this strain received its genes from H9N2, H7N9 and H5N6 AIVs infecting poultry in China. This strain showed moderate ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3219-2
更新日期:2017-05-01 00:00:00
abstract::Retroviral gag proteins, as well as fragments minimally containing the capsid (CA) and nucleocapsid (NC) subunits of Gag, are able to spontaneously assemble into virus-like particles (VLPs). This occurs in mammalian and bacterial cells as well as in in vitro systems. In every circumstance, nucleic acids are incorporat...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1873-6
更新日期:2014-04-01 00:00:00
abstract::Two cases of coinfection with bovine papular stomatitis virus (BPSV) and pseudocowpox virus (PCPV) in dairy calves in Tochigi Prefecture, Japan, are reported. Sequences of BPSV and PCPV were simultaneously detected in the same polymerase chain reaction (PCR) amplicons, which were obtained from the DNA of two dairy cal...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04792-3
更新日期:2020-11-01 00:00:00
abstract::Cell-mediated immune response was studied in experimental herpetic infection in mice. Our data suggest that herpes simplex virus induced immunosuppression in mice. This depression was documented by the unresponsiveness of the lymphocytes to PHA stimulation in vitro and by the enhanced susceptibility of adult mice to C...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF02175597
更新日期:1975-01-01 00:00:00
abstract::In this work, we describe the complete sequence and genome organization of a novel tobamovirus detected in a prickly pear plant (Opuntia sp.) by high-throughput sequencing, tentatively named "opuntia virus 2". The full genome of opuntia virus 2 is 6,453 nucleotides in length and contains four open reading frames (ORFs...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04528-3
更新日期:2020-03-01 00:00:00
abstract::Here, we report a novel virulent P2-like bacteriophage, R18C, isolated from rabbit faeces, which, in addition to Escherichia coli K-12 strains, was able to be propagated on Citrobacter rodentium strain ICC169 and a range of Shigella sonnei strains with high efficiency of plating (EOP). It represents the first lytic ba...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04424-5
更新日期:2019-12-01 00:00:00
abstract::Analysis of the preS1 gene of hepatitis B virus was used to define two nosocomial outbreaks of HBV infection. In an outbreak in an oncology unit we had previously shown by single stranded conformational polymorphism (SSCP) analysis of a 189 bp fragment of the preS1 gene, that 52 children were infected with HBV strains...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050200
更新日期:1997-01-01 00:00:00
abstract::"Phages" include viruses of eubacteria and archaea. At least 5568 phages have been examined in the electron microscope since the introduction of negative staining in 1959. Most virions (96%) are tailed. Only 208 phages (3.7%) are polyhedral, filamentous, or pleomorphic. Phages belong to one order, 17 families, and thr...
journal_title:Archives of virology
pub_type: 杂志文章,评审
doi:10.1007/s00705-006-0849-1
更新日期:2007-02-01 00:00:00
abstract::Rabbit anti-idiotypic antibodies (anti-ids) were generated against three bank vole and one human monoclonal antibody (MAb) specific for the two envelope glycoproteins of Puumala virus (G1 and G2). The anti-ids were purified by sequential immunoaffinity chromatography. Each anti-id inhibited the antigen binding of its ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309537
更新日期:1993-01-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) causes a variety of cancers, including Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman disease (MCD). Host cellular microRNAs (miRNAs) play important post-transcriptional regulatory roles in gene expression and can greatly influence viru...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3478-y
更新日期:2017-11-01 00:00:00
abstract::When 66 cucurbit samples with yellowing symptoms from fields in Mali, the Philippines, Thailand and Uzbekistan were screened by RT-PCR using universal polerovirus primers, 21 were identified as harboring polerovirus RNA. When these 21 samples were screened with specific primers for the known cucurbit-infecting polerov...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1939-5
更新日期:2014-06-01 00:00:00