Abortive infection of influenza virus in Ehrlich ascites tumor cells. Unusual fragility of virus particles.

Abstract:

:Noninfectious virus particles were produced in Ehrlich ascites tumor cells infected intraperitoneally with fowl plague virus. The PFU yield of virus per cell was less than 0.1 and the ratio PFU/HA units in the progeny virus was less than 10(3). The virus particles had the same morphology and size as egg-grown virus but were more fragile. They were disrupted by centrifugation through sucrose and caesium chloride gradients, but this disruption was avoided by fixing the particles with formaldehyde before centrifugation. Analysis of polypeptides by SDS-PAGE showed that ascites-grown virus particles contained reduced amounts of matrix protein compared with egg-grown virus.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Bukrinskaya AG,Gitelman AK,Martynenko VB

doi

10.1007/BF01315278

subject

Has Abstract

pub_date

1978-01-01 00:00:00

pages

279-90

issue

4

eissn

0304-8608

issn

1432-8798

journal_volume

56

pub_type

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