Preparation and characterization of monoclonal antibodies to enteric adenovirus types 40 and 41.

Abstract:

:We have prepared monoclonal antibodies to each of the enteric adenoviruses types 40 and 41. Three different hybridoma cell lines were selected which produced antibody found to react by radioimmunoprecipitation with adenovirus (Ad) hexon antigens. One was specific for Ad 40, another for Ad 41, and a third one reacted with both types. When tested in an enzyme immunoassay against all 41 known human Ad types, the type-specific monoclonal antibody against Ad 40 reacted homotypically, as did the monoclonal antibody against Ad 41. In addition, these monoclonal antibodies neutralized the homologous enteric Ad type. The monoclonal antibody which reacted with both enteric Ad types also showed lower levels of reactivity with the group C adenoviruses types 2, 5, and 6. The monoclonal antibodies produced will provide a definitive means for rapid identification of specific Ad types, and will be useful in defining the relationship of enteric adenoviruses to other types.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Herrmann JE,Perron-Henry DM,Stobbs-Walro D,Blacklow NR

doi

10.1007/BF01310718

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

259-65

issue

3-4

eissn

0304-8608

issn

1432-8798

journal_volume

94

pub_type

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