Abstract:
:We describe an unexpected feature observed for the heterologous expression of the Thyrinteina arnobia cypovirus polyhedrin from a recombinant baculovirus infection in different insect cell lines. The in cellulo-formed crystals varied in size and shape depending on the cell line. Crystals formed in Trichoplusia ni-derived cells were cubic (0.1-2 μm) and localized in both the nucleus and cytoplasm, whereas those formed in Spodoptera frugiperda-derived cells were ovate and ellipsoidal (0.1-3 μm) and also localized in both the nucleus and cytoplasm. The molecular basis for differences in the morphology, size, and location of cypovirus occlusion bodies is unclear, and cellular proteins might play a role in their formation and location.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Silva LA,Ardisson-Araújo DMP,Morgado FS,Horta AB,Lemos MVF,Wilcken CF,Ribeiro BMdoi
10.1007/s00705-019-04214-zsubject
Has Abstractpub_date
2019-06-01 00:00:00pages
1677-1682issue
6eissn
0304-8608issn
1432-8798pii
10.1007/s00705-019-04214-zjournal_volume
164pub_type
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