Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus.

Abstract:

:We describe an unexpected feature observed for the heterologous expression of the Thyrinteina arnobia cypovirus polyhedrin from a recombinant baculovirus infection in different insect cell lines. The in cellulo-formed crystals varied in size and shape depending on the cell line. Crystals formed in Trichoplusia ni-derived cells were cubic (0.1-2 μm) and localized in both the nucleus and cytoplasm, whereas those formed in Spodoptera frugiperda-derived cells were ovate and ellipsoidal (0.1-3 μm) and also localized in both the nucleus and cytoplasm. The molecular basis for differences in the morphology, size, and location of cypovirus occlusion bodies is unclear, and cellular proteins might play a role in their formation and location.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Silva LA,Ardisson-Araújo DMP,Morgado FS,Horta AB,Lemos MVF,Wilcken CF,Ribeiro BM

doi

10.1007/s00705-019-04214-z

subject

Has Abstract

pub_date

2019-06-01 00:00:00

pages

1677-1682

issue

6

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-019-04214-z

journal_volume

164

pub_type

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