Abstract:
:We constructed a recombinant baculovirus encoding a dengue (DEN)-2 virus envelope glycoprotein truncated of 102 amino acids (aa) at its C-terminus (D2E delta 102). The production, processing and transportation of the recombinant protein in baculovirus-infected Spodoptera frugiperda (Sf9) cells and its immunogenic properties in mice were compared to those of a previously characterized recombinant DEN-2 E-protein with a 71aa C-terminal truncation (D2E delta 71). Both proteins were transported through the Golgi complex and their N-oligosaccharides of the high mannose type were processed to the complex mannose type. D2E delta 102 transited to the plasma membrane and was secreted whereas D2E delta 71 presumably remained associated with the plasma membrane. The reactivities of the recombinant proteins with neutralizing monoclonal antibodies were similar. Both intracellular and extracellular D2E delta 102 induced neutralizing antibodies in mice and were thus immunogenic. The level of protective immunity to DEN-2 virus encephalitis challenge in mice vaccinated with intracellular D2E delta 102 (80%, p < 0.01) was lower than that induced with D2E delta 71 (90%, P < 0.001). Sixty-eight percent (P < 0.001) of mice vaccinated with 5 micrograms of extracellular D2E delta 102 protein were protected against lethal challenge.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Delenda C,Frenkiel MP,Deubel Vdoi
10.1007/BF01309465subject
Has Abstractpub_date
1994-01-01 00:00:00pages
197-207issue
1-2eissn
0304-8608issn
1432-8798journal_volume
139pub_type
杂志文章abstract::During the course of hominoid evolution, a new transcript variant of the GSDML (gasdermin-like protein) gene was formed by the integration of the antisense-oriented HERV-H (human endogenous retrovirus) LTR (long terminal repeat) element. To investigate regions that are critical for transcriptional regulation of the GS...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0105-y
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01347975
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abstract::Tobacco vein banding mosaic virus (TVBMV) is of increasing importance in tobacco production. Knowledge of the genetic structure and variability of the virus population is vital for developing sustainable management. In this study, 24 new TVBMV isolates from Sichuan Province together with 46 previous isolates were stud...
journal_title:Archives of virology
pub_type: 杂志文章
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abstract::We constructed a recombinant feline herpesvirus type 1 (FHV-1) which was deleted in a defined region (450 bp) within the thymidine kinase (TK) gene (C7301dlTK) [Yokoyama et al. (1995) J Vet Med Sci 57: 709-714]. In this report, we carried out two experiments to assess the pathogenicity and vaccine efficacy of the reco...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718312
更新日期:1996-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-008-0152-4
更新日期:2008-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1118-5
更新日期:2011-12-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310965
更新日期:1985-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718644
更新日期:1996-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-04-01 00:00:00
abstract::Hepatitis E virus (HEV) genotype 3 is an emerging pathogen in the developed world. As the clinical manifestations and routine laboratory parameters are often nonspecific, accurate diagnostic tests are crucial. In the current study, the performance of six serological assays and three PCR assays for the detection of HEV...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3395-0
更新日期:2017-09-01 00:00:00
abstract::Wild plants of the family Convolvulaceae are hosts for a few New World begomoviruses (genus Begomovirus, family Geminiviridae). In this work, we report the complete genome sequence of a new begomovirus infecting the wild convolvulaceous plant Jacquemontia tamnifolia in Venezuela. The cloned bipartite genome showed the...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3372-7
更新日期:2017-08-01 00:00:00
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doi:10.1007/s00705-020-04945-4
更新日期:2021-01-24 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01322663
更新日期:1995-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3446-6
更新日期:2017-10-01 00:00:00
abstract::A virus, named Oya virus, was isolated in Vero cell cultures from the lungs of a pig suspected of Nipah virus infection. The virus was revealed as a spherical enveloped RNA virus with a diameter of 79 nm. For identification of Oya virus, RT-PCR was performed. A common primer set for S-RNA of the Simbu serogroup of the...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0838-y
更新日期:2002-08-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-011-1034-8
更新日期:2011-09-01 00:00:00
abstract::A cross-sectional study was undertaken during 2012-2013 to determine the prevalence, strains and factors associated with rotavirus infection among under-5-year-old children hospitalized with acute diarrhea in Uganda. Rotaviruses were detected in 37 % (263/712) of the children. The most prevalent strains were G9P[8] (2...
journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:2016-04-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04792-3
更新日期:2020-11-01 00:00:00
abstract::The alteration of whole Sendai virus and especially of its nucleocapsid polypeptides, during storage of the virus at 4 degree C in the allantoic fluids in which it was cultivated, has cultivated, has been studied by sodium dodecyl sulfate gel electrophoresis. During virus storage the nucleocapsid protein subunits with...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317427
更新日期:1975-01-01 00:00:00
abstract::The oncogene E6 of human papillomavirus 8, which is associated with skin cancers in epidermodysplasia verruciformis, was transcribed and translated in vitro. The resulting 17 kDa protein did not bind to the cellular p53 in contrast to E6 of HPV16. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309654
更新日期:1992-01-01 00:00:00
abstract::Human polymorphonuclear leukocytes (PMNL) incubated with influenza virus, A/USSR/90/77 (H1N1) or its hemagglutinin produced a significant increase in their PKC activity when compared with untreated PMNL. The activated kinase translocated from cytosol to cellular membrane. The calcium-dependent enzyme activity was inhi...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050439
更新日期:1998-01-01 00:00:00
abstract::Hemagglutinating and acetylesterase functions as well as the 124 kDa glycoprotein were present in the highly cell-culture adapted, avirulent bovine coronavirus strain BCV-L9, in the Norden vaccine strain derived from it, and in 5 wild-type, virulent strains that multiplied in HRT-18 cells but were restricted in severa...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309637
更新日期:1992-01-01 00:00:00
abstract::Noninfectious virus particles were produced in Ehrlich ascites tumor cells infected intraperitoneally with fowl plague virus. The PFU yield of virus per cell was less than 0.1 and the ratio PFU/HA units in the progeny virus was less than 10(3). The virus particles had the same morphology and size as egg-grown virus bu...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315278
更新日期:1978-01-01 00:00:00
abstract::Alfamo- and ilarviruses are characterized by the deficiency of their genomes (three messenger-sense RNAs) to start an infection cycle. The RNAs are in capsids built from a single species of protein of about 24 kD. A few dimers of this coat protein per RNA molecule are sufficient to activate the genome. Since the first...
journal_title:Archives of virology
pub_type: 杂志文章,评审
doi:10.1007/s007050050551
更新日期:1999-01-01 00:00:00
abstract::Murine monoclonal antibodies against the fusion (F) and hemagglutinin-neuraminidase (HN) proteins of Sendai virus (SV) were prepared and studied on their antiviral activities, particularly on the neutralization of infectivity. On the analysis with solid phase competitive ELISA, 26 anti-HN antibodies were divided into ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01316735
更新日期:1986-01-01 00:00:00
abstract::Peritoneal exudate cells (PEC) of DBA/2 mice, after 7 days of in vitro preculture and consisting of virtually 100 per cent macrophages, were able to support the replication of Herpes Simplex Virus type 1 strain WAL (HSV). Using a standard medium based on Dulbecco's Modified Eagle Medium (D-MEM), no virus replication w...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309370
更新日期:1984-01-01 00:00:00
abstract::In infants, hepatitis B virus (HBV) infections are mainly acquired by mother-to-child transmission (MTCT). Current tests for the presence of HBV markers at birth can neither confirm nor exclude MTCT. The aim of this study was to find an early diagnostic marker of HBV MTCT. From 2011 to 2016, we studied a total of 5999...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04190-4
更新日期:2019-05-01 00:00:00
abstract::H9N2 avian influenza virus has caused huge economic loss for the Chinese poultry industry since it was first identified. Vaccination is frequently used as a control method for the disease. Meanwhile suspension culture has become an important tool for the development of influenza vaccines. To optimize the suspension cu...
journal_title:Archives of virology
pub_type: 临床试验,杂志文章
doi:10.1007/s00705-017-3460-8
更新日期:2017-10-01 00:00:00