Abstract:
:We focused on the role of membrane bound sugar residues in the infection of fibroblasts and monocyte-like cells with human cytomegalovirus (HCMV). Treatment of phorbol 12-myristate 13-acetate (PMA) differentiated monocyte-like cells THP-1 or human fibroblasts MRC-5 with lectins specific for N-acetylneuraminic acid (NeuAc) blocked infection with HCMV. HCMV failed to infect sialidase-treated differentiated THP-1 cells or MRC-5 cells. By using NeuAc, N-glycolylneuraminic acid (NeuGl) and alpha 2-3, but not alpha 2-6, sialyl-oligosaccharide, the infection of cells was less efficient. NeuAc was more potent inhibitor than NeuGl. These observations suggest that the sialic acid specificity and the nature of the interglycosidic linkage at the end of the complex carbohydrates may play an important role. Analogous experiments indicated that HCMV binds to N-acetylglucosamine (GlcNAc) in addition to NeuAc. Human cytomegalovirus infection in differentiated THP-1 cells and in human fibroblasts was inhibited by incubation of the virus with 20 micrograms/ml of heparin before and during the adsorption period. Treatment of the cells with heparinase or heparitinase inhibited infection with HCMV. We emphasized the role of NeuAc and GlcNAc and heparan sulfate proteoglycans at the surface of the cells, in the early steps of infection of both human fibroblasts and PMA differentiated monocyte-like cells with HCMV.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Lobert PE,Hober D,Dewilde A,Wattré Pdoi
10.1007/BF01322663subject
Has Abstractpub_date
1995-01-01 00:00:00pages
1357-71issue
8eissn
0304-8608issn
1432-8798journal_volume
140pub_type
杂志文章abstract::The complete nucleotide sequence of the ssRNA genome of a Chinese GPV isolate of barley yellow dwarf virus (BYDV) was determined. It comprised 5673 nucleotides, and the deduced genome organization resembled that of members of the genus Polerovirus. It was most closely related to cereal yellow dwarf virus-RPV (77% nt i...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0415-8
更新日期:2009-01-01 00:00:00
abstract::The nucleotide sequences of the entire F genes of two isolates of the pigeon PMV-1 (PPMV-1) variant of Newcastle disease virus (NDV) were determined using RTPCR. The deduced amino acid sequences of the F0 protein showed four differences between isolate 760/83 which had been passaged 4 times in chickens and gave an int...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718322
更新日期:1996-01-01 00:00:00
abstract::Nine pestiviruses isolated from different batches of a contaminated Tunisian sheep pox vaccine and one Tunisian field ovine isolate of border disease virus (BDV) were studied at the antigenic and molecular levels. Seroneutralization tests were carried out on three vaccine isolates, the Tunisian field isolate and repre...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0427-3
更新日期:2005-02-01 00:00:00
abstract::Mice are protected against lethal intraperitoneal and intravenous infection by encephalomyocarditis virus and Semliki Forest virus by sequential treatment with poly I followed by either polyC or poly5-hydroxyC without production of interferon when the treatments are 4 or more hours apart and by the intraperitoneal or ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318024
更新日期:1976-01-01 00:00:00
abstract::Cassava mosaic geminiviruses (CMGs) are implicated in cassava mosaic disease (CMD), the main constraint to cassava production in Africa. Here, we report the complete nucleotide sequences of the DNA-A and DNA-B of a newly characterized CMG found infecting cassava in Madagascar, for which we propose the tentative name c...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1399-3
更新日期:2012-10-01 00:00:00
abstract::In 2007, African swine fever virus (ASFV) was introduced into the Transcaucasian countries and Russia. Since then, it has spread alarmingly and reached the European Union. ASFV strains are highly virulent and lead to almost 100% mortality under experimental conditions. However, the possibility of dose-dependent diseas...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2430-2
更新日期:2015-07-01 00:00:00
abstract::The plaque size and morphology of twenty-two influenza A virus recombinants representing seven distinct families were analyzed on MDCK cells. By examination of the genetic composition of the recombinants no relationship could be established between any gene, including those coding for the surface antigens, and the pla...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314716
更新日期:1982-01-01 00:00:00
abstract::A distinct strain of hollyhock yellow vein mosaic virus (HoYVMV) and Ludwigia leaf distortion betasatellite (LuLDB) were associated with yellow vein mosaic of hollyhock. The viral DNA genome (JQ911766) and betasatellite (JQ408216) shared highest nucleotide sequence identity (89.2 %) with HoYVMV (the only available seq...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2108-1
更新日期:2014-10-01 00:00:00
abstract::Since the first rescue of a recombinant Newcastle disease virus (rNDV) in the late 1990s, many more rNDVs have been rescued by researchers around the world. Regardless of methodology, the main principle behind rescue of the virus has remained the same, i.e., the formation of a functional replication complex by simulta...
journal_title:Archives of virology
pub_type: 杂志文章,评审
doi:10.1007/s00705-016-3065-7
更新日期:2017-01-01 00:00:00
abstract::We have examined by immunofluorescent antibody staining technique the expression of herpes simplex virus type 1 (HSV-1) in organ cultures of the normal human oral mucosa. The expression of HSV-1 antigen was found selectively in the epithelial cell layers in relatively undifferentiated states such as basal layer and lo...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310061
更新日期:1987-01-01 00:00:00
abstract::In previous work, we demonstrated that the arenavirus Junín virus (JUNV) is able to activate Akt by means of the phosphatidylinositol-3-kinase (PI3K) survival pathway during virus entry. This work extends our study, emphasizing the relevance of this pathway in the establishment and maintenance of persistent infection ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2298-6
更新日期:2015-02-01 00:00:00
abstract::Rabbit kidney epithelial cells (RK13) exhibited a cytopathic effect (CPE) characterized by cell rounding after infection with human cytomegalovirus (HCMV). Although HCMV-specific immediate early and early antigens were detected by indirect immunofluorescence techniques, neither late antigens nor infectious progeny vir...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314121
更新日期:1985-01-01 00:00:00
abstract::A comparison of 15 field isolates of grapevine leafroll-associated virus 5 (GLRaV-5) was conducted, based on the analysis of nucleotide sequences of two viral ORFs: the coat protein (CP) and the heat shock protein 90 homolog (HSP90h). After amplification and cloning, the population of viral sequences was analyzed for ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1371-2
更新日期:2012-09-01 00:00:00
abstract::A virus isolated from Olea europaea L. grown in Portugal, was identified as a member of the species Tobacco necrosis virus D (TNV-D, genus Necrovirus, family Tombusviridae), based on the molecular and serological properties of the purified virus particles. The genomic region encoding the coat protein (CP) of this isol...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0258-7
更新日期:2004-06-01 00:00:00
abstract::Human bocavirus (HBoV)-2, a new parvovirus, has been identified in stool samples and is suggested to be one of the etiologic agents of acute gastroenteritis (GE). The purpose of this study was to investigate the prevalence of HBoV-2 in children with GE. Stool samples were collected from 358 children hospitalized with ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-009-0533-3
更新日期:2009-01-01 00:00:00
abstract::Virus-like particles (VLP), morphologically similar to togavirus particles, were detected in the sera of chimpanzees and humans with chronic non-A, non-B (NANB) hepatitis. Particles resembling the virus were also found in the cytoplasm of hepatocytes obtained from NANB hepatitis infected chimpanzees. Furthermore, two ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01315557
更新日期:1989-01-01 00:00:00
abstract::Here, we report a novel virulent P2-like bacteriophage, R18C, isolated from rabbit faeces, which, in addition to Escherichia coli K-12 strains, was able to be propagated on Citrobacter rodentium strain ICC169 and a range of Shigella sonnei strains with high efficiency of plating (EOP). It represents the first lytic ba...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-019-04424-5
更新日期:2019-12-01 00:00:00
abstract::A physical map for the genome of Spodoptera littoralis (Egyptian cottonworm) nuclear polyhedrosis virus of B-type (S1MNPV-B) [Cherry CL, Summers MD (1985) J Invertebr Pathol 46: 289-295] was prepared using the restriction endonucleases SmaI, SfiI, NotI, ApaI, KpnI, BstEII, PstI, SacI, and HindIII. The size of the geno...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01313815
更新日期:1989-01-01 00:00:00
abstract::A new virus belonging to the family Dicistroviridae was identified in the hibiscus-infesting cotton mealybug Phenacoccus solenopsis. Using high-throughput sequencing (HTS) on an Illumina HiSeq platform, a single contig of the complete genome sequence was assembled. The authenticity of the sequence obtained by HTS was ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04702-7
更新日期:2020-09-01 00:00:00
abstract::Human cytomegalovirus (HCMV), oxidized by sodium periodate (NaIO4), is incapable of giving rise to viral progeny in cell culture. At a NaIO4 concentration as low as 5 mM, there is a loss of at least 6 logs of viral infectivity which occurs very rapidly (less than 5 min). Further, the inactivation is a first-order reac...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309765
更新日期:1994-01-01 00:00:00
abstract::We have recently demonstrated the geographic isolation of rice tungro bacilliform virus (RTBV) populations in the tungro-endemic provinces of Isabela and North Cotabato, Philippines. In this study, we examined the genetic structure of the virus populations at the tungro-outbreak sites of Lanao del Norte, a province ad...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050070013
更新日期:2000-01-01 00:00:00
abstract::The yeast two-hybrid system has been used to identify domains of the Newcastle disease virus (NDV) phosphoprotein (P) involved in self-association and interaction with the nucleocapsid protein (NP). Deletion analysis was used to map the domain(s) of the P protein involved in P:P and P:NP interactions. The C-terminal 4...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0439-z
更新日期:2005-03-01 00:00:00
abstract::A study was made of the phylogenetic relationships between fifteen complete nucleotide sequences as well as 43 nucleotide sequences of the putative coat protein gene of different strains belonging to the virus species Beak and feather disease virus obtained from 39 individuals of 16 psittacine species. The species inc...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0368-x
更新日期:2004-12-01 00:00:00
abstract::Two cases of coinfection with bovine papular stomatitis virus (BPSV) and pseudocowpox virus (PCPV) in dairy calves in Tochigi Prefecture, Japan, are reported. Sequences of BPSV and PCPV were simultaneously detected in the same polymerase chain reaction (PCR) amplicons, which were obtained from the DNA of two dairy cal...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04792-3
更新日期:2020-11-01 00:00:00
abstract::We investigated the potential of Nicotiana benthamiana to express the E7 protein of human papillomavirus 8 (HPV-8), a paradigm genotype among cutaneous HPVs. The protein, modified in its putative pRb-binding domain (E7(QGD)), was transiently expressed in leaves following infiltration with agrobacteria carrying either ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-010-0893-8
更新日期:2011-04-01 00:00:00
abstract::By means of indirect immunofluorescence assay (IFA) using natural swine immune serum and hyperimmune serum from rabbits infected with porcine circovirus (PCV), a PCV antigen was detected present prior to the onset of viral and cellular DNA synthesis in nucleoli of cells of synchronized and growth stimulated infected P...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01384343
更新日期:1995-01-01 00:00:00
abstract::The effect of the local anaesthetic drug procaine on the adsorption of two enveloped viruses was studied. Physiological concentrations of the drug (7 X 10(-3)--7 X 10(-2) M) strongly inhibited the adsorption of both West-Nile and herpes viruses as determined by plaque assay and the infective center assay. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317291
更新日期:1978-01-01 00:00:00
abstract::Usurpation of the host's signalling pathways is a common strategy employed by viruses to promote their successful replication. Here we show that infection with the orthopoxvirus vaccinia virus (VACV) leads to sustained stimulation of c-Jun activity during the entire infective cycle. This stimulation is temporally regu...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3446-6
更新日期:2017-10-01 00:00:00
abstract::Infectious bursal disease virus (IBDV), a member of the family Birnaviridae, is a non-enveloped, double-stranded RNA virus. Viral protein 1 (VP1), the putative RNA-dependent RNA polymerase, occurs in virions both as a free polypeptide and as a genome-linked protein, called VPg. To gain more insight in its function, we...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0365-0
更新日期:2004-11-01 00:00:00
abstract::There were three epidemic waves of human infection with avian influenza A (H7N9) virus in 2013-2014. While many analyses of the genomic origin, evolution, and molecular characteristics of the influenza A (H7N9) virus have been performed using sequences from the first epidemic wave, genomic characterization of the viru...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-2872-1
更新日期:2016-08-01 00:00:00