Abstract:
:Rabbit kidney epithelial cells (RK13) exhibited a cytopathic effect (CPE) characterized by cell rounding after infection with human cytomegalovirus (HCMV). Although HCMV-specific immediate early and early antigens were detected by indirect immunofluorescence techniques, neither late antigens nor infectious progeny virus could be observed in virus-infected RK13 cells. HCMV-infected RK13 cells showed a prolonged doubling time and a decreased saturation density in cell growth compared to uninfected control cells. Moreover, colony forming ability (CFA) of virus-infected cells decreased by approximately 70 per cent compared to that of uninfected cells during the first 24 hours after infection. These results indicate that an abortive infection of RK13 cells with HCMV induces an alteration of growth pattern including morphological changes with cytocidal effect.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Kamiya S,Tanaka J,Ogura T,Sato H,Ogura H,Yoshie T,Hatano Mdoi
10.1007/BF01314121subject
Has Abstractpub_date
1985-01-01 00:00:00pages
143-50issue
1-2eissn
0304-8608issn
1432-8798journal_volume
86pub_type
杂志文章abstract::Strains of an influenza H10N4 virus have been isolated during an outbreak of a respiratory disease in mink on the south-east coast of Sweden. This was the first example of a disease in mammals caused by the H10 subtype. We compared the A/mink/Sweden/84 strain with two recent avian H10N4 isolates, one from fowl and ano...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01318353
更新日期:1990-01-01 00:00:00
abstract::DNA polymerase (DNApol) is present in all baculoviruses and plays a crucial role in viral DNA replication. Previously we showed that the DNApol of the alphabaculovirus group II Spodoptera litura nucleopolyhedrovirus (SpltNPV) could partially substitute for the DNApol of a group I alphabaculovirus, Autographa californi...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-017-3468-0
更新日期:2017-11-01 00:00:00
abstract::The DNAs from nine Japanese field isolates of equine herpesvirus-1 (EHV-1) were analyzed by digestion with the restriction endonuclease Bam HI and Southern hybridization. Comparing restriction profiles among the EHV-1 strains, there was no considerable difference between isolates before and after vaccine application, ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01316881
更新日期:1993-01-01 00:00:00
abstract::Two herpes simplex type 1 (HSV-1) recombinant viruses carrying the hepatitis C virus (HCV) NS5A open reading frame under the control of the cytomegalovirus immediate early (IE) or a herpes simplex chimeric promoter (alpha 4 gamma 1UL19) were constructed and characterized. Expression studies showed that both HSV-NS5A r...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-002-0925-0
更新日期:2003-02-01 00:00:00
abstract::We determined the complete nucleotide sequence of a broad bean wilt virus 2 (BBWV-2) isolate from gentian in Japan. The full-length RNA1 and RNA2 sequences, excluding poly(A) tails, were 5955 and 3600 nucleotides long, respectively. Analysis indicated that, in contrast to other BBWV-2 isolates, the 5' end of both RNA1...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1625-7
更新日期:2013-07-01 00:00:00
abstract::Big liver and spleen disease, caused by avian hepatitis E virus, has been reported in Poland, but the prevalence of the virus has not yet been investigated. In this study, 1034 serum samples from 57 breeder broiler and laying hen flocks were screened for the presence of anti-aHEV antibodies. In a random serology study...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-018-4089-y
更新日期:2019-02-01 00:00:00
abstract::An arracacha (Arracacia xanthorrhiza) plant collected in Brazil was found to be infected by a new virus. This viral isolate (named C17) systemically infected Nicotiana benthamiana and Apium graveolens. A polyclonal antibody was raised, and analysis of our arracacha germplasm collection showed a high infection rate of ...
journal_title:Archives of virology
pub_type: 杂志文章,评审
doi:10.1007/s00705-008-0289-1
更新日期:2009-01-01 00:00:00
abstract::Hemorrhagic fever with renal syndrome (HFRS) is endemic in East Asia and Europe. The disease is caused by several viruses belonging to the genus Hantavirus, including the Hantaan virus (HTNV), Seoul virus (SEOV), Dobrava Belgrade virus (DOBV), and Puumala virus (PUUV). Recently, HTNV-related viruses, Amur (AMR) and Fa...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0113-x
更新日期:2003-08-01 00:00:00
abstract::DNA of TT virus (TTV), a novel human circovirus, was tested for in 116 mother-infant pairs who had participated in the adult T-cell leukemia prevention program (APP) in Nagasaki, Japan, and refrained from breast-feeding. By polymerase chain reaction with Okamoto's seminested primers, 36 of the 115 (31%) mothers were p...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050044
更新日期:2000-01-01 00:00:00
abstract::Viral infection is usually initiated by the binding of virus particles to specific receptor molecule(s) on the host cell surface. Blocking of this step prevents the following step, penetration into the cell. In the present study, we investigated the virus-cell interactions of virions of Norwalk virus (NV), a major eti...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-003-0143-4
更新日期:2003-09-01 00:00:00
abstract::We determined the genome sequence of a new polerovirus that infects field pea and faba bean in China. Its entire nucleotide sequence (6021 nt) was most closely related (83.3% identity) to that of an Ethiopian isolate of chickpea chlorotic stunt virus (CpCSV-Eth). With the exception of the coat protein (encoded by ORF3...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1301-3
更新日期:2012-07-01 00:00:00
abstract::Ljungan virus is a recently identified member of the family Picornaviridae that was isolated from bank voles in Sweden. LjV has been associated with [corrected] type 1 diabetes-like symptoms and myocarditis in bank voles (Myodes glareolus), and it has been suggested that it has zoonotic potential. Here, we show for th...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1731-6
更新日期:2014-03-01 00:00:00
abstract::The plaque size and morphology of twenty-two influenza A virus recombinants representing seven distinct families were analyzed on MDCK cells. By examination of the genetic composition of the recombinants no relationship could be established between any gene, including those coding for the surface antigens, and the pla...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314716
更新日期:1982-01-01 00:00:00
abstract::In order to differentiate recent isolates of avian infectious bronchitis virus (IBV) in Taiwan, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and direct sequencing methods were used to type 25 IBV Taiwan isolates. Two conserved sequences that flank the hypervariable region I (HVR I)...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718291
更新日期:1996-01-01 00:00:00
abstract::It is shown that reovirus capsid inner layer consists of 132 capsomeres with a skewed icosahedral pattern of T = 13 (left). 780 structural units composing capsomeres are integrated into trimers. The boundary between capsomeres runs between the structural units forming trimers. ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01313903
更新日期:1987-01-01 00:00:00
abstract::Mosquito-borne alphaviruses are widely distributed throughout the world, causing important human illnesses. Therefore, the development of methods to enable early diagnosis of infections by alphavirus is essential. We show here the development and evaluation of a quantitative real-time RT-PCR using genus-specific prime...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-016-3019-0
更新日期:2016-11-01 00:00:00
abstract::Agarose in situ digestion was used to prepare intact Amsacta moorei entomopoxvirus (AmEPV) DNA from embedded occlusion bodies (OBs). Direct dissolution of OBs in agarose eliminated the necessity for separate purification of virions. A physical map of AmEPV DNA was constructed for five restriction enzymes (BamHI, EcoRI...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310704
更新日期:1990-01-01 00:00:00
abstract::We have previously demonstrated that HIV-infected transformed T-cells or monocytes adhere to monolayers of CD4-negative epithelial cells. Adhesion is soon followed by budding of HIV from infected mononuclear cells onto the surface of epithelial cells. Epithelial cells subsequently take up virus and become productively...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718823
更新日期:1996-01-01 00:00:00
abstract::The presence of transmitted HIV drug resistance (TDR) threatens the efficacy of antiretroviral treatment. We aimed to assess the changes in TDR prevalence over the last decade in Madrid, Spain, to verify the role of the patients' risk groups in these changes. We analysed the trends of TDR between 2000 and 2011 in a co...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-013-1933-y
更新日期:2014-05-01 00:00:00
abstract::Highly pathogenic avian influenza (HPAI) virus subtype H5N1 was first reported in Myanmar in 2006. In this study, we have characterized 6 HPAI (H5N1) viruses recovered from 2007-2010 as well as three additional available nucleotide sequences representing Myanmar AI outbreaks. Phylogenetic analysis showed that the Myan...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1411-y
更新日期:2012-11-01 00:00:00
abstract::The alkaloids contained in Colchicum autumnale seeds are used in numerous medicines. Good quality seeds are difficult to obtain from this undomesticated plant. Therefore, a research program was set up aiming to cultivate C. autumnale in order to improve alkaloid contents and seed yields. In this context, a collection ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0307-x
更新日期:2004-07-01 00:00:00
abstract::A porcine teschovirus (PTV) was isolated from a dead piglet from a herd of 200 sows showing reproductive failure in Fuyu, Heilongjiang Province, China. Sequencing of most of the genome of this isolate, designated Fuyu/2009, revealed that is was a PTV-8 isolate, closely related to a previously identified Chinese PTV-8 ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-012-1315-x
更新日期:2012-07-01 00:00:00
abstract::To provide information on the mechanism of attenuation of a Newcastle disease vaccine strain, TCND, we compared it with the parental virulent strain California 11,914 (CAL) biologically and genetically. It was found that TCND bore the fusion protein of virulent type, consisting of a pair of dibasic amino acid residues...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050361
更新日期:1998-01-01 00:00:00
abstract::The nucleotide sequence of genomic RNA of Marburg virus strain Popp was determined. Strain Popp was isolated in 1967 during the first filoviral outbreak. The virus was purified from blood of infected guinea pigs in which it had been maintained. The length of the determined sequence was 19112 nucleotides. Amino acid se...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01322532
更新日期:1995-01-01 00:00:00
abstract::The Sindbis replicon expression system was used to express La Crosse (LAC) virus envelope glycoprotein genes in both mammalian and mosquito cell culture. Replicon expressed LAC proteins had correct molecular mass (Mr) and were antigenically similar to wild type LAC envelope proteins. In addition, LAC G1 and G2 protein...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050381
更新日期:1998-01-01 00:00:00
abstract::Gp 85 the major envelope glycoprotein of Rous sarcoma virus can be released from intact by virus treatment with 2-mercaptoethanol. Attempts were made to reassociate released gp 85 to 2-mercaptoethanol treated virus. In order to study the biological activity and to analyse the reaction product with biochemical methods ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314883
更新日期:1982-01-01 00:00:00
abstract::A simple radial method based on the formerly described paper disc technique was developed for demonstration of neutralizing antibodies to enteroviruses with plaque forming ability. Sera administered by 5 microliter wire-loops to wells cut into the agar overlay of virus infected cell monolayers produced radial neutrali...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01317320
更新日期:1980-01-01 00:00:00
abstract::The highly conserved encapsidation signal (epsilon) of hepatitis B viral (HBV) pregenomic RNA has been reported as an essential component for encapsidation and protein priming of HBV polymerase. Here, we report that two HBV epsilon RNA-binding host proteins (80 and 43 kDa) and a copurifying protein (100 kDa) were puri...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050200001
更新日期:2002-03-01 00:00:00
abstract::Both H9N2 subtype avian influenza and 2009 pandemic H1N1 viruses (pH1N1) can infect humans and pigs, which provides the opportunity for virus reassortment, leading to the genesis of new strains with potential pandemic risk. In this study, we generated six reassortant H9 viruses in the background of three pH1N1 strains...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-014-2009-3
更新日期:2014-07-01 00:00:00
abstract::A novel myovirus, vB_PagM_AAM22 (AAM22), was isolated in Lithuania using Pantoea agglomerans as the host for phage propagation. The 49,744-bp genome of AAM22 has a G + C content of 48.4% and contains 96 probable protein-encoding genes and no genes for tRNA. In total, 34 ORFs were given a putative functional annotation...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04705-4
更新日期:2020-09-01 00:00:00