Abstract：
:Agarose in situ digestion was used to prepare intact Amsacta moorei entomopoxvirus (AmEPV) DNA from embedded occlusion bodies (OBs). Direct dissolution of OBs in agarose eliminated the necessity for separate purification of virions. A physical map of AmEPV DNA was constructed for five restriction enzymes (BamHI, EcoRI, HindIII, PstI, and XhoI) using single and multiple digests, and isolated fragment digestions. End fragments were identified by Bal31 digestion and snap-back analysis. A least squares procedure was used to reconcile fragment lengths. AmEPV genome size estimates were based on restriction enzyme (REN) fragment length totals (222 kb), reconciled physical map distance (225 kb), and field inversion gel electrophoresis (FIGE) (about 242 kb). Presumably due to the high A + T content (18.5% G + C) of AmEPV DNA, FIGE values for the intact genome and large REN fragments were about 6 to 10% higher than expected. Preparative FIGE was used to concentrate AmEPV DNA from agarose microbead encapsulated insect cells (Estigmene acrea, BTI-EAA). REN digests of this DNA were identical to those from OBs from caterpillars.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Hall RL,Hink WFdoi
10.1007/BF01310704subject
Has Abstractpub_date
1990-01-01 00:00:00pages
77-90issue
1-2eissn
0304-8608issn
1432-8798journal_volume
110pub_type
杂志文章abstract：:MicroRNAs (miRNAs) are a new class of well-conserved small noncoding RNAs that mediate posttranscriptional gene regulation. Hepatitis B virus (HBV) causes various liver diseases, including chronic hepatitis, liver cirrhosis and hepatocellular cancer. Recent data have indicated HBV alters miRNAs expression patterns, bu...
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journal_title：Archives of virology
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更新日期：1991-01-01 00:00:00
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更新日期：1998-01-01 00:00:00
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journal_title：Archives of virology
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doi：10.1007/BF01315090
更新日期：1978-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00705-015-2621-x
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pub_type： 杂志文章
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更新日期：1988-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF01314237
更新日期：1985-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
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更新日期：1984-01-01 00:00:00
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journal_title：Archives of virology
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更新日期：1982-01-01 00:00:00
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journal_title：Archives of virology
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更新日期：1978-01-01 00:00:00
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更新日期：1986-01-01 00:00:00
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更新日期：1984-01-01 00:00:00
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更新日期：1984-01-01 00:00:00
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更新日期：2019-12-01 00:00:00
