Demonstration of non-infectious hemagglutinating particles of rabies virus and isolation of the hemagglutinin by disruption of the virion with Nonidet P-40.

Abstract:

:Non-infectious hemagglutinating particles of rabies virus accumulated in the fluid phase of chick embryo cell cultures at 6 days post-infection, though they were undetectable at 4 days. They were characterized as looped filaments resembling viral envelope as revealed by electron microscopy. Another form of hemagglutinin (HAnin) was obtained by solubilization of partially purified virions with Nonidet P-40 (NP-40) followed by successive high speed and CsCl density gradient centrifugations. The density of the isolated HAnin averaged 1.28 g/cm3. SDS-polyacrylamide gel electrophoresis of the HAnin demonstrated that it was mainly composed of a glycoprotein (G) with a molecular weight of 83,000. Electron microscopically, it differed from the above non-infectious hemagglutinating particles, being much smaller in size and showing a star- or rosette-like appearance with a diameter of about 25 nm, composed of a central particle surrounded by particles resembling envelope-spikes. Virus-neutralizing (VN) and hemagglutination inhibiting (HI) antibodies were produced in rabbits immunized with the HAnin isolated from virions.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Arai YT,Kondo A,Suzuki K

doi

10.1007/BF01317937

subject

Has Abstract

pub_date

1976-01-01 00:00:00

pages

335-45

issue

4

eissn

0304-8608

issn

1432-8798

journal_volume

51

pub_type

杂志文章
  • Further molecular characterisation of potyviruses infecting aroid plants for medicinal use in China.

    abstract::Degenerate primers were used to detect and amplify 3'-terminal genome fragments of potyviruses from medicinal aroid plants growing at 16 sites in China. Virus was detected in 7 samples of which six, all of Pinellia ternata, contained a strain of soybean mosaic virus (SMV) similar to that previously reported from this ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-004-0390-z

    authors: Shi YH,Hong XY,Chen J,Adams MJ,Zheng HY,Lin L,Qin BX,Chen JP

    更新日期:2005-01-01 00:00:00

  • Characterization of the structural proteins of hemorrhagic enteritis virus.

    abstract::The structural proteins of hemorrhagic enteritis (HEV), a turkey adenovirus, were analyzed by polyacrylamide gel electrophoresis (PAGE) and Western blotting using polyspecific, monospecific and monoclonal antibodies for detection. In purified HEV preparations, eleven polypeptides with apparent molecular weights rangin...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01309695

    authors: van den Hurk JV

    更新日期:1992-01-01 00:00:00

  • Cloning and nucleotide sequencing of the S4 genome segment of avian reovirus S1133.

    abstract::The sequence of RNA genome segment S4 of the avian reovirus (ARV) strain S1133 was determined. S4 RNA is 1185 base pairs long and contains one open reading frame encoding a protein of 367 amino acid residues (40.6 kDa), the similar size as the known S4 gene product (sigma NS), with a net charge of -1 at neutral pH. Th...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s007050050258

    authors: Chiu CJ,Lee LH

    更新日期:1997-01-01 00:00:00

  • Identification of a third member of the Anellovirus genus ("small anellovirus") in French blood donors.

    abstract::We demonstrate for the first time that a putative third member of the genus Anellovirus (TTV/TTMV) is present in the blood of healthy persons (20% prevalence), and also in their PBMNC and saliva samples. ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-005-0660-4

    authors: Biagini P,de Micco P,de Lamballerie X

    更新日期:2006-02-01 00:00:00

  • Complete nucleotide sequence of loquat virus A, a member of the family Betaflexiviridae with a novel genome organization.

    abstract::Analysis of a loquat tree with leaf curl symptoms by deep sequencing revealed a novel virus with a single-stranded RNA genome, for which the name "loquat virus A" (LoVA) was proposed. The complete genome sequence comprised 7553 nucleotides (nt) and an additional poly(A) tail at the 3' terminus. Sequence comparisons of...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-019-04444-1

    authors: Liu Q,Yang L,Xuan Z,Wu J,Qiu Y,Zhang S,Wu D,Zhou C,Cao M

    更新日期:2020-01-01 00:00:00

  • Pattern of anti-cytomegalovirus IgM antibodies determined by immunoblotting. A study of kidney graft recipients developing a primary or recurrent CMV infection.

    abstract::In order to improve the knowledge of the humoral immune response to CMV infection, we developed an immunoblotting technique which allowed a better analysis of the changes in the pattern of anti CMV-polypeptides IgM. We examined 234 sera belonging to 27 renal allograft recipients developing a primary or recurrent CMV i...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01310938

    authors: Basson J,Tardy JC,Aymard M

    更新日期:1989-01-01 00:00:00

  • Parvovirus (feline panleucopaenia virus) plaque formation.

    abstract::A plaque assay was developed for feline parvovirus (FPV; feline panleucopaenia virus) in a feline embryo (FEmb) cell line. Higher numbers and larger diameter plaques were obtained with a) seeding rates of 0.7 X 10(5) and 1.5 X 10(5) cells cf. 3 X 10(5) and 6 X 10(5) cells/well of 35 mm diameter, b) synchronised cells ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01378978

    authors: Tham KM,Studdert MJ

    更新日期:1985-01-01 00:00:00

  • Phylogenetic relationships and the occurrence of interspecific recombination between beet chlorosis virus (BChV) and Beet mild yellowing virus (BMYV).

    abstract::Samples containing two viruses belonging to the genus Polerovirus, beet chlorosis virus (BChV) and beet mild yellowing virus (BMYV), were collected from French and Polish sugar beet fields. The molecular properties of 24 isolates of BChV and BMYV were investigated, and their genetic diversity was examined in the coat ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-014-2245-6

    authors: Kozlowska-Makulska A,Hasiow-Jaroszewska B,Szyndel MS,Herrbach E,Bouzoubaa S,Lemaire O,Beuve M

    更新日期:2015-02-01 00:00:00

  • Conservation of epitopes recognized by monoclonal antibodies against the separated subunits of influenza hemagglutinin among type A viruses of the same and different subtypes.

    abstract::Monoclonal antibodies raised against the separated hemagglutinin subunits (HA1 and HA2) of influenza A/Vic/3/75 (H3N2) virus were tested against a large panel of human and avian strains. The epitopes recognized by most antibodies were conserved among subtype H3 viruses, but reactivity of some antibodies with members o...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01319250

    authors: Sanchez-Fauquier A,Guillen M,Martin J,Kendal AP,Melero JA

    更新日期:1991-01-01 00:00:00

  • Study of the polymerization step of the rolling circle replication of peach latent mosaic viroid.

    abstract::We have developed an in vitro transcriptional assay using Escherichia coli RNA polymerase to initiate the replication of peach latent mosaic viroid (PLMVd). Regardless of the polarity of the PLMVd strand used as template, initiation in vitro occurred at the same hairpin structure. These initiation sites correspond to ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s007050170061

    authors: Pelchat M,Coté F,Perreault JP

    更新日期:2001-01-01 00:00:00

  • Course and transmission characteristics of oral low-dose infection of domestic pigs and European wild boar with a Caucasian African swine fever virus isolate.

    abstract::In 2007, African swine fever virus (ASFV) was introduced into the Transcaucasian countries and Russia. Since then, it has spread alarmingly and reached the European Union. ASFV strains are highly virulent and lead to almost 100% mortality under experimental conditions. However, the possibility of dose-dependent diseas...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-015-2430-2

    authors: Pietschmann J,Guinat C,Beer M,Pronin V,Tauscher K,Petrov A,Keil G,Blome S

    更新日期:2015-07-01 00:00:00

  • Inactivation of hepatitis C virus cDNA transgene by hypermethylation in transgenic mice.

    abstract::Transgenic mice were produced by microinjection of a partial hepatitis C virus (HCV) genome sequence including the structural protein region, under the control of the albumin promoter and enhancer into fertilized eggs of C57BL/6 and BDF1 mice. Three founders carrying at least five copies of the transgene but not expre...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01718169

    authors: Kato T,Ahmed M,Yamamoto T,Takahashi H,Oohara M,Ikeda T,Aida Y,Katsuki M,Arakawa Y,Shikata T,Esumi M

    更新日期:1996-01-01 00:00:00

  • Immune adherence hemagglutination test for detection of antibody to respiratory syncytial virus. Brief report.

    abstract::An immune adherence hemagglutination test was established for detection of antibodies to respiratory syncytial virus. The test was shown to be as specific as and much more sensitive than the neutralization and complement fixation tests. ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01309371

    authors: Kobayashi N,Noguchi Y,Kojima M,Matumoto M

    更新日期:1984-01-01 00:00:00

  • Determination of the 5' and 3' terminal noncoding sequences of the bi-segmented genome of the avibirnavirus infectious bursal disease virus.

    abstract::Terminal sequences of the bi-segmented dsRNA genome of 3 different strains of infectious bursal disease virus (IBDV) were analyzed by the rapid amplification of cDNA 5' ends (5'RACE) procedure. Both segments are 85% homologous in a 32-nucleotide sequence comprising the 5' end, whereas the 3' end has a conserved pentam...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01718616

    authors: Kibenge FS,Nagarajan MM,Qian B

    更新日期:1996-01-01 00:00:00

  • Genetic properties of endemic Chinese porcine epidemic diarrhea virus strains isolated since 2010.

    abstract::Acute diarrhea outbreaks caused by porcine epidemic diarrhea virus (PEDV) have been observed in various pig-breeding provinces of China since December 2010. Endemic strains of PEDV were isolated from different areas, and the complete genome sequences of 10 isolates were determined. Our objective in this study was to g...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-013-1767-7

    authors: Wang XM,Niu BB,Yan H,Gao DS,Yang X,Chen L,Chang HT,Zhao J,Wang CQ

    更新日期:2013-12-01 00:00:00

  • Properties of human adenoviruses 5 and 7 grown in guinea-pig cells.

    abstract::Human adenoviruses (Ad) 5 and 7 multiplied poorly in guinea-pig cell cultures as compared to HeLa cells. Ad 7 had a modified polypeptide composition with a major 43K component. This change persisted for up to 4 passages in HeLa cells. Ad 5 was not changed by passage in guinea-pig cells. ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01320631

    authors: Thomasset N,Chardonnet Y

    更新日期:1980-01-01 00:00:00

  • An emerging novel bovine coronavirus with a 4-amino-acid insertion in the receptor-binding domain of the hemagglutinin-esterase gene.

    abstract::The hemagglutinin-esterase (HE) protein of betacoronavirus lineage A is a secondary receptor in the infection process and is involved in the emergence of new betacoronavirus genotypes with altered host specificity and tissue tropism. We previously reported a novel recombinant bovine coronavirus (BCoV) strain that was ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-020-04840-y

    authors: Abi KM,Zhang Q,Zhang B,Zhou L,Yue H,Tang C

    更新日期:2020-12-01 00:00:00

  • First detection of wheat streak mosaic virus in Germany: molecular and biological characteristics.

    abstract::Wheat streak mosaic virus is a serious threat in wheat-producing countries. In Germany, the virus was first recorded in 2013 near Hoym. The complete sequence of isolate Hoym was obtained and compared to all other known complete WSMV sequences, including newly collected and sequenced isolates from France and Austria. P...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-015-2422-2

    authors: Schubert J,Ziegler A,Rabenstein F

    更新日期:2015-07-01 00:00:00

  • Complete genome sequence of a novel sub-subgenotype 2.1g isolate of classical swine fever virus from China.

    abstract::Current subgenotype 2.1 isolates of classical swine fever virus (CSFV) play a dominant role in CSF outbreaks in China, and a novel sub-subgenotype 2.1g of CSFV was recently identified, but the complete genome sequence of this new sub-subgenotype has not been reported. In this study, complete genome of 2.1g isolate GD1...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-016-2932-6

    authors: Gong W,Zhang L,Lu Z,Jia J,Wang M,Peng Z,Guo H,Shi J,Tu C

    更新日期:2016-09-01 00:00:00

  • Relative reactivity of the V3 loop PND of HIV-1 subtypes A, B, C, D, and F with sera from selected Ugandan localities.

    abstract::Synthetic peptides comprising the predicted principal neutralizing determinant (PND) in new African and North American HIV-1 clones were tested in ELISA for reactivity with ninety six serum samples from asymptomatic donors in six selected localities in Uganda. Irrespective of the geographical origin of the samples, th...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01322666

    authors: Riley JP,Pestano GA,Hosford K,Francis C,Xie JM,Mugyenyi P,Kataaha P,Katongole-Mbidde E,Anokbonggo WW,Guyden J

    更新日期:1995-01-01 00:00:00

  • Detection of canine astrovirus in dogs with diarrhea in Japan.

    abstract::Canine astrovirus (CAstV) is the causative agent of gastroenteritis in dogs. We collected rectal swabs from dogs with or without diarrhea symptoms in Japan and examined the feces for the presence of CAstV by RT-PCR with primers based on a conserved region of the ORF1b gene. The ORF1b gene of CAstV was not detected in ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-015-2405-3

    authors: Takano T,Takashina M,Doki T,Hohdatsu T

    更新日期:2015-06-01 00:00:00

  • Identification, transcription and sequence analysis of the Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) DNA polymerase gene.

    abstract::Sequence analysis of a 6.4 kb DNA region from the Spodoptera littoralis multinucleocapsid nucleopolyhedrovirus (SpliNPV) revealed a large open reading frame (ORF) encoding a predicted polypeptide of 998 amino acid (aa) residues with a molecular mass of 114.93 kDa, located between 47.2-52.3 m.u. on the SpliNPV genome. ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s007050170177

    authors: Huang J,Levin DB

    更新日期:2001-01-01 00:00:00

  • Asystasia mosaic Madagascar virus: a novel bipartite begomovirus infecting the weed Asystasia gangetica in Madagascar.

    abstract::Here, we describe for the first time the complete genome sequence of a new bipartite begomovirus in Madagascar isolated from the weed Asystasia gangetica (Acanthaceae), for which we propose the tentative name asystasia mosaic Madagascar virus (AMMGV). DNA-A and -B nucleotide sequences of AMMGV were only distantly rela...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-015-2413-3

    authors: De Bruyn A,Harimalala M,Hoareau M,Ranomenjanahary S,Reynaud B,Lefeuvre P,Lett JM

    更新日期:2015-06-01 00:00:00

  • Avian hepatitis E virus is widespread among chickens in Poland and belongs to genotype 2.

    abstract::Big liver and spleen disease, caused by avian hepatitis E virus, has been reported in Poland, but the prevalence of the virus has not yet been investigated. In this study, 1034 serum samples from 57 breeder broiler and laying hen flocks were screened for the presence of anti-aHEV antibodies. In a random serology study...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-018-4089-y

    authors: Matczuk AK,Ćwiek K,Wieliczko A

    更新日期:2019-02-01 00:00:00

  • Molecular characterization of two genotypes of a new polerovirus infecting brassicas in China.

    abstract::The genomic RNA sequences of two genotypes of a brassica-infecting polerovirus from China were determined. Sequence analysis revealed that the virus was closely related to but significantly different from turnip yellows virus (TuYV). This virus and other poleroviruses, including TuYV, had less than 90% amino acid sequ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-011-1091-z

    authors: Xiang HY,Dong SW,Shang QX,Zhou CJ,Li DW,Yu JL,Han CG

    更新日期:2011-12-01 00:00:00

  • Functional analysis of the heterologous NSP1 genes in the genetic background of simian rotavirus SA11.

    abstract::Function of rotavirus NSP1 was analyzed by using single-NSP1 gene-substitution reassortants, SKF, SDF, and SNF which have the NSP1 gene derived from human rotaviruses KU, DS-1, and canine rotavirus K9, respectively, in the genetic background of simian rotavirus SA11. The NSP1 genes from KU, DS-1, K9, and SA11 exhibite...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s007050050600

    authors: Okada J,Kobayashi N,Taniguchi K,Shiomi H

    更新日期:1999-01-01 00:00:00

  • The complete nucleotide sequence of Subterranean clover mottle virus.

    abstract::The complete nucleotide sequence of Subterranean clover mottle virus (SCMoV) genomic RNA has been determined. The SCMoV genome is 4,258 nucleotides in length. It shares most nucleotide and amino acid sequence identity with the genome of Lucerne transient streak virus (LTSV). SCMoV RNA encodes four overlapping open rea...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-003-0144-3

    authors: Dwyer GI,Njeru R,Williamson S,Fosu-Nyarko J,Hopkins R,Jones RA,Waterhouse PM,Jones MG

    更新日期:2003-11-01 00:00:00

  • Canarypox and fowlpox viruses as recombinant vaccine vectors: an ultrastructural comparative analysis.

    abstract::Due to their natural host-range restriction to avian species, canarypox virus (CP) and fowlpox virus (FP) represent efficient and safe vaccine vectors, as they correctly express transgenes in human cells, elicit complete immune responses, and show protective efficacy in preclinical animal models. At present, no inform...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/s00705-010-0663-7

    authors: Pacchioni S,Volonté L,Zanotto C,Pozzi E,De Giuli Morghen C,Radaelli A

    更新日期:2010-06-01 00:00:00

  • Isolation of a recombinant influenza virus (Hsw 1 N2) from swine in Japan.

    abstract::Outbreaks of swine influenza were first observed in Japan in 1978. A number of influenza viruses were isolated from diseased swine. Almost all viruses isolated were swine influenza virus (Hsw 1 N1) but two viruses isolated from the nasal swabs of swine showing clinical signs of influenza in the Kanagawa prefecture wer...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01314741

    authors: Sugimura T,Yonemochi H,Ogawa T,Tanaka Y,Kumagai T

    更新日期:1980-01-01 00:00:00

  • Mechanism of entry of a xenotropic MMuLV-derived recombinant retrovirus into porcine cells using the expression of the reporter nlslacZ gene.

    abstract::A xenotropic Moloney murine leukemia virus-derived recombinant retrovirus (MMuLVSVnlslacZ) has been utilized to study the mechanism of virus entry into endothelial and epithelial porcine cells. In the genome of this recombinant retrovirus, the nlslacZ reporter gene is under the transcriptional control of both LTR and ...

    journal_title:Archives of virology

    pub_type: 杂志文章

    doi:10.1007/BF01314625

    authors: Gilbert MA,Charreau B,Vicart P,Paulin D,Nandi PK

    更新日期:1992-01-01 00:00:00