Abstract:
:Non-infectious hemagglutinating particles of rabies virus accumulated in the fluid phase of chick embryo cell cultures at 6 days post-infection, though they were undetectable at 4 days. They were characterized as looped filaments resembling viral envelope as revealed by electron microscopy. Another form of hemagglutinin (HAnin) was obtained by solubilization of partially purified virions with Nonidet P-40 (NP-40) followed by successive high speed and CsCl density gradient centrifugations. The density of the isolated HAnin averaged 1.28 g/cm3. SDS-polyacrylamide gel electrophoresis of the HAnin demonstrated that it was mainly composed of a glycoprotein (G) with a molecular weight of 83,000. Electron microscopically, it differed from the above non-infectious hemagglutinating particles, being much smaller in size and showing a star- or rosette-like appearance with a diameter of about 25 nm, composed of a central particle surrounded by particles resembling envelope-spikes. Virus-neutralizing (VN) and hemagglutination inhibiting (HI) antibodies were produced in rabbits immunized with the HAnin isolated from virions.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Arai YT,Kondo A,Suzuki Kdoi
10.1007/BF01317937subject
Has Abstractpub_date
1976-01-01 00:00:00pages
335-45issue
4eissn
0304-8608issn
1432-8798journal_volume
51pub_type
杂志文章abstract::Degenerate primers were used to detect and amplify 3'-terminal genome fragments of potyviruses from medicinal aroid plants growing at 16 sites in China. Virus was detected in 7 samples of which six, all of Pinellia ternata, contained a strain of soybean mosaic virus (SMV) similar to that previously reported from this ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-004-0390-z
更新日期:2005-01-01 00:00:00
abstract::The structural proteins of hemorrhagic enteritis (HEV), a turkey adenovirus, were analyzed by polyacrylamide gel electrophoresis (PAGE) and Western blotting using polyspecific, monospecific and monoclonal antibodies for detection. In purified HEV preparations, eleven polypeptides with apparent molecular weights rangin...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309695
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abstract::The sequence of RNA genome segment S4 of the avian reovirus (ARV) strain S1133 was determined. S4 RNA is 1185 base pairs long and contains one open reading frame encoding a protein of 367 amino acid residues (40.6 kDa), the similar size as the known S4 gene product (sigma NS), with a net charge of -1 at neutral pH. Th...
journal_title:Archives of virology
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journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01310938
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journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:1985-01-01 00:00:00
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更新日期:2015-02-01 00:00:00
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journal_title:Archives of virology
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更新日期:1991-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718169
更新日期:1996-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01309371
更新日期:1984-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01718616
更新日期:1996-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01320631
更新日期:1980-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-020-04840-y
更新日期:2020-12-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s00705-015-2422-2
更新日期:2015-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/s007050050600
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journal_title:Archives of virology
pub_type: 杂志文章
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更新日期:2010-06-01 00:00:00
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journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314741
更新日期:1980-01-01 00:00:00
abstract::A xenotropic Moloney murine leukemia virus-derived recombinant retrovirus (MMuLVSVnlslacZ) has been utilized to study the mechanism of virus entry into endothelial and epithelial porcine cells. In the genome of this recombinant retrovirus, the nlslacZ reporter gene is under the transcriptional control of both LTR and ...
journal_title:Archives of virology
pub_type: 杂志文章
doi:10.1007/BF01314625
更新日期:1992-01-01 00:00:00