Abstract：
:The isolation of ortho- and paramyxovirus glycoproteins using a new nonionic detergent (MESK) is reported. MESK was shown to solubilize most of the viral envelope glycoproteins without decreasing their biologic activity. Solubilized glycoproteins are not contaminated by any internal viral proteins or by appreciable quantities of viral envelope lipids. The removal of MESK by dialysis resulted in the formation of glycoprotein micelles. The immunogenic activity of isolated glycoproteins was compared to that of virus particles. Immunization with isolated glycoproteins was shown to protect mice against a lethal influenza infection. Virions were treated with MESK in the presence of exogenous egg phosphatidylcholine, detergent was removed by dialysis and the glycoprotein was reconstituted in the vesicles. This reconstitution was accompanied by restoration of the haemolytic activity of Sendai virus proteins up to that of native virus particles. The level of activity, also the morphology and buoyant density of the vesicle were dependent on the protein/lipid ratio. MESK proved to be of value for the selective solubilization of the surface glycoproteins of animal enveloped viruses and their reconstitution in liposomes.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Berezin VA,Zaides VM,Artamonov AF,Isaeva ES,Zhdanov VMdoi
10.1007/BF01314237subject
Has Abstractpub_date
1985-01-01 00:00:00pages
281-300issue
3-4eissn
0304-8608issn
1432-8798journal_volume
85pub_type
杂志文章abstract：:In February 2008, five patients were infected with the H1N1 subtype of influenza A virus in one hospital ward for immunocompromised patients at a hospital in North Rhine-Westphalia, Germany. All of these patients had an established haematologic disease and tested positive either for viral RNA or antigen shortly after ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0771-4
更新日期：2010-11-01 00:00:00
abstract：:We studied the kinetics of localisation of matrix (M) protein of Human respiratory syncytial virus (RSV) in infected cells. M protein was detected in the nucleus early in infection, by confocal microscopy and by immunoblotting of nuclear fractions. We next tested the possibility that M protein may be involved in inhib...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-003-0112-y
更新日期：2003-07-01 00:00:00
abstract：:Norovirus (NoV) infection is the most common cause of nonbacterial acute gastroenteritis, which affects both adults and children. However, the molecular epidemiology of NoV in adults with acute gastroenteritis in China has not been investigated extensively. In this study, we investigated the occurrence of NoV infectio...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2440-0
更新日期：2015-07-01 00:00:00
abstract：:Strains of an influenza H10N4 virus have been isolated during an outbreak of a respiratory disease in mink on the south-east coast of Sweden. This was the first example of a disease in mammals caused by the H10 subtype. We compared the A/mink/Sweden/84 strain with two recent avian H10N4 isolates, one from fowl and ano...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318353
更新日期：1990-01-01 00:00:00
abstract：:RNA2, the short segment of the genome of Dicenthrarchus labrax encephalitis virus (DIEV), a fish nodavirus causing seabass encephalitis, was cloned. Sequence analysis revealed that DIEV RNA2 contains a single open reading frame (ORF), which carries the catalytic D-75 residue but lacks the site for autocatalytic proteo...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050248
更新日期：1997-01-01 00:00:00
abstract：:Serology for parvovirus B19 has been hampered by limited availability of antigen which has often had to be isolated from viraemic blood donations. We have determined the sequence of the genome of one such isolate (Stu). It is 99% similar to the sequences of two other isolates (Wi and Au) except at the far 5'-end, wher...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718833
更新日期：1996-01-01 00:00:00
abstract：:The complete genome of ϕ16, a temperate corynephage from Corynebacterium glutamicum ATCC 21792, was sequenced and annotated (GenBank: KY250482). The electron microscopy study of ϕ16 virion confirmed that it belongs to the family Siphoviridae. The ϕ16 genome consists of a linear double-stranded DNA molecule of 58,200 b...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3383-4
更新日期：2017-08-01 00:00:00
abstract：:A type III Trichomonas vaginalis virus, which may be involved in transcriptional regulation of the major surface protein gene P270 of the protozoan pathogen Trichomonas vaginalis, was purified and characterized in the present study. The complete 4844-base-pair complementary DNA sequence of the viral genome reveals ove...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-010-0858-y
更新日期：2011-02-01 00:00:00
abstract：:Insertion of foreign DNA containing the E. coli gpt marker by homologous recombination in the pigeonpox virus (PPV) thymidine kinase (TK) gene and selection for the presence of this DNA in the viral genome produced unstable recombinants after 3 plaque purifications. We highlight the persistence of duplicated TK DNA se...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01378643
更新日期：1993-01-01 00:00:00
abstract：:Alfamo- and ilarviruses are characterized by the deficiency of their genomes (three messenger-sense RNAs) to start an infection cycle. The RNAs are in capsids built from a single species of protein of about 24 kD. A few dimers of this coat protein per RNA molecule are sufficient to activate the genome. Since the first...
journal_title：Archives of virology
pub_type： 杂志文章,评审
doi：10.1007/s007050050551
更新日期：1999-01-01 00:00:00
abstract：:The nucleotide sequences of the entire F genes of two isolates of the pigeon PMV-1 (PPMV-1) variant of Newcastle disease virus (NDV) were determined using RTPCR. The deduced amino acid sequences of the F0 protein showed four differences between isolate 760/83 which had been passaged 4 times in chickens and gave an int...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718322
更新日期：1996-01-01 00:00:00
abstract：:Rice transitory yellowing virus (RTYV), a member of the genus Nucleorhabdovirus, is closely related to or synonymous with rice yellow stunt virus (RYSV). To clarify the relationship between RTYV and RYSV, we determined the nucleotide sequence of the RTYV genome. The RTYV genome consists of 14,029 nucleotides. The over...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0557-8
更新日期：2010-02-01 00:00:00
abstract：:Herpes simplex virus type 2 (HSV-2), strain YS-4 C-1, isolated by plaque cloning from a clinical isolate was found to be resistant to acyclovir (ACV; acycloguanosine) in vitro. It was sensitive to phosphonoacetic acid and 9-beta-D-arabinofuranosyladenine. Thymidine kinase (TK) activity of YS-4 C-1 was less than 1% of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310998
更新日期：1988-01-01 00:00:00
abstract：:Vector transmission is an important part of the viral infection cycle, yet for many viruses little is known about this process, or how viral sequence variation affects transmission efficacy. Here we examined the effect of substituting genes from the highly transmissible FS577 isolate of citrus tristeza virus (CTV) in ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3070-x
更新日期：2016-12-01 00:00:00
abstract：:Receptor-mediated activation is accompanied by phospholipid metabolism and by calcium fluctuation resulting in a chemiluminescence (CL) response in the neutrophil. This pathway involves activation of protein kinase C (PKC) and the NADPH oxidase. Artificial stimulants such as phorbol esters, specifically 12-O-tetradeca...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310506
更新日期：1990-01-01 00:00:00
abstract：:The interaction between the nucleocapsid (N) protein of mouse hepatitis virus (MHV) and RNA was studied in an effort to define portions of the N molecule that participate in binding to RNA. N mRNAs transcribed from SP6 and T7 vectors were translated in a rabbit reticulocyte lysate. Analysis of synthesized N protein in...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309634
更新日期：1992-01-01 00:00:00
abstract：:The thymidine kinase (TK) of herpesviruses, in contrast to cellular TKs, phosphorylates a variety of substrates including antiherpetic nucleoside analogues. This study reports the identification and DNA sequence of the simian varicella virus (SVV) TK gene. A 32P-labeled varicella zoster virus (VZV) TK DNA probe hybrid...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718587
更新日期：1996-01-01 00:00:00
abstract：:In this study, we describe a novel mycovirus isolated from Trichoderma asperellum, which was designated Trichoderma asperellum dsRNA Virus 1 (TaRV1). The sequence analysis revealed that TaRV1 has two discontinuous open reading frames (ORF), ORF1 and ORF2. A hypothetical protein and an RNA-dependent RNA polymerase are ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3988-2
更新日期：2018-12-01 00:00:00
abstract：:Human metapneumovirus (hMPV) is an important respiratory pathogen in young children whose seasonal activity varies substantially from year to year among different populations. This study was conducted to investigate if there was a seasonal variation in the incidence of hMPV infection in young children and possible ass...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1492-7
更新日期：2013-02-01 00:00:00
abstract：:A putative chrysovirus recovered from Brassica campestris var. purpurea and provisionally named "Brassica campestris chrysovirus 1" (BrcCV1) was sequenced. The genome of the putative BrcCV1 consists of three double-stranded RNAs (dsRNAs) comprising 3,639 (dsRNA 1), 3,567 (dsRNA 2) and 3,337 (dsRNA 3) base pairs, respe...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3196-x
更新日期：2017-04-01 00:00:00
abstract：:Foot-and-mouth disease virus (FMDV) is the most contagious pathogen in cloven-hoofed (two-toed) animals. Due to the rapid replication and spread of FMDV, novel therapeutic strategies are greatly needed to reduce or block FMDV shedding in cases of disease outbreak. Here, we generated an IRES-Mx1 construct in which the ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2473-4
更新日期：2015-08-01 00:00:00
abstract：:Human papillomaviruses (HPVs) are responsible for the most common human sexually transmitted viral infections. Infection with high-risk HPVs, particularly HPV16, is associated with the development of cervical cancer. The papillomavirus L1 major capsid protein, the basis of the currently marketed vaccines, self-assembl...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-009-0484-8
更新日期：2009-01-01 00:00:00
abstract：:A non-neutralizing monoclonal antibody (YO-60) against human rotavirus was found to be directed to VP2 (90,000-dalton protein), one of the two major components of the inner capsid. The reactivity patterns of the YO-60 antibody were very similar, though not identical, to those of subgroup II-specific YO-5 monoclonal an...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310550
更新日期：1986-01-01 00:00:00
abstract：:Genetic heterogeneous mouse populations selected for high (HIII) and low (LIII) antibody response were used to study some aspects of mouse hepatitis virus 3 (MHV3) infection, such as the resistance pattern, virus replication in the liver and peritoneal exudate or in cultured peritoneal macrophages, the interferon (IFN...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309475
更新日期：1994-01-01 00:00:00
abstract：:Human diploid BAMB cells with epitheloid morphology, which had been derived from amniotic fluid cells, were capable of supporting the replication of human cytomegalovirus (CMV), without prior treatment of the cells with halogenated pyrimidines. The growth of this virus in BAMB cells and in human diploid fibroblastoid ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315617
更新日期：1976-01-01 00:00:00
abstract：:Beet necrotic yellow vein virus RNA 1 contains a single long ORF corresponding to the theoretical translation product of 237 kDa which contains the information necessary for replication of the viral genome. This ORF contains a putative papain-like proteinase domain which has been localized, on the basis of sequence al...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050141
更新日期：1997-01-01 00:00:00
abstract：:Genomic characterization of Norwalk-like human caliciviruses (NLVs) originating from outbreaks and sporadic cases of acute gastroenteritis has revealed surprisingly high levels of diversity, even in the RNA polymerase gene, which is anticipated to be highly conserved. Since information on antigenic relationship is lim...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050020
更新日期：2000-01-01 00:00:00
abstract：:B-cell epitopes of varicella-zoster virus glycoprotein II were mapped by means of solid phase ELISA, synthetic oligopeptides (constructed according to the Davison-Scott sequencing of the varicella-zoster virus genome) and sera from varicellae and herpes zoster patients. The individual pattern of antibody peptide bindi...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01718644
更新日期：1996-01-01 00:00:00
abstract：:In this study, RNA corresponding to bovine enterovirus (BEV) was detected in 24.6 % of faecal samples (17/69) from diarrheic and healthy cattle in six different areas in China by an RT-PCR screening method. Furthermore, two cytopathic agents, designated as BHM26 and BJ50, were isolated from the bovine diarrheic fecal ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1424-6
更新日期：2012-12-01 00:00:00
abstract：:A porcine teschovirus (PTV) was isolated from a dead piglet from a herd of 200 sows showing reproductive failure in Fuyu, Heilongjiang Province, China. Sequencing of most of the genome of this isolate, designated Fuyu/2009, revealed that is was a PTV-8 isolate, closely related to a previously identified Chinese PTV-8 ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1315-x
更新日期：2012-07-01 00:00:00
