The genome sequence of Brassica campestris chrysovirus 1, a novel putative plant-infecting tripartite chrysovirus.

Abstract:

:A putative chrysovirus recovered from Brassica campestris var. purpurea and provisionally named "Brassica campestris chrysovirus 1" (BrcCV1) was sequenced. The genome of the putative BrcCV1 consists of three double-stranded RNAs (dsRNAs) comprising 3,639 (dsRNA 1), 3,567 (dsRNA 2) and 3,337 (dsRNA 3) base pairs, respectively, each containing a single open reading frame (ORF 1-3). The putative proteins encoded by ORF 1-3 show homologies to RdRp, CP and chryso-P3 of approved or tentative chrysoviruses. In addition, the three dsRNAs of BrcCV1 contain highly conserved 5' and 3' untranslated regions (UTRs) in a way similar to known chrysoviruses. In a phylogenetic tree based on the conserved amino acid sequences of the RdRps of chrysoviruses, totiviruses and partitiviruses, the putative BrcCV1 formed a separate clade with Raphanus sativus chrysovirus 1 (RasCV1), a putative trisegmented, plant-infecting chrysovirus, in the family Chrysoviridae.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Zhang J,Zhao Z,Hu R,Guo L,Zheng L,Du Z,Wu Z,Fang S,Zhang S,Liu Y

doi

10.1007/s00705-016-3196-x

subject

Has Abstract

pub_date

2017-04-01 00:00:00

pages

1107-1111

issue

4

eissn

0304-8608

issn

1432-8798

pii

10.1007/s00705-016-3196-x

journal_volume

162

pub_type

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