Abstract：
:Transgenic mice were produced by microinjection of a partial hepatitis C virus (HCV) genome sequence including the structural protein region, under the control of the albumin promoter and enhancer into fertilized eggs of C57BL/6 and BDF1 mice. Three founders carrying at least five copies of the transgene but not expressing HCV-specific RNA were generated. Methylation analysis indicated that the transgene was extensively methylated. Mapping of methylated cytosine residues of the transgenic mouse DNA showed that all C residues of a particular part of the HCV genome but not all the CpG island like sequences were methylated. Transiently expressed HCV cDNA in COS7 cells and the active endogenous albumin gene were not methylated. Furthermore, 5-azacytidine, a potent demethylating agent, induced HCV gene expression in a line of these transgenic mice. These results suggest that methylation of HCV cDNA is a cause of its inactive expression in transgenic mice, and that this phenomenon may occur in other stable systems for expression of the HCV genome.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Kato T,Ahmed M,Yamamoto T,Takahashi H,Oohara M,Ikeda T,Aida Y,Katsuki M,Arakawa Y,Shikata T,Esumi Mdoi
10.1007/BF01718169subject
Has Abstractpub_date
1996-01-01 00:00:00pages
951-8issue
5eissn
0304-8608issn
1432-8798journal_volume
141pub_type
杂志文章abstract：:Immunofluorescence assay using monospecific and monoclonal antibodies to the 65 K major protein of human cytomegalovirus (HCMV) was carried out to monitor the expression of this protein in infected cells. Regardless of differences in the reactivity of the monoclonal antibodies, as determined by immunoblotting and immu...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01314653
更新日期：1988-01-01 00:00:00
abstract：:Virus isolate Y3V, obtained from tobacco showing leaf curl symptoms in Yunnan, China, had particles with the size and morphology typical of geminiviruses. In reactions with a set of monoclonal antibodies raised against begomoviruses, Y3V was readily differentiated from two previously studied Chinese Begomovirus isolat...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170081
更新日期：2001-08-01 00:00:00
abstract：:Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by the SFTS virus (SFTSV). Hubei Province is a major epidemic area for SFTS in China. In this study, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and serological testing (IgM) were used simultaneously ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-3993-5
更新日期：2018-12-01 00:00:00
abstract：:A full-length cDNA clone of olive latent virus 1 (OLV-1), a member of the genus Necrovirus, family Tombusviridae, was subjected to site-directed mutagenesis, and coat protein gene mutants were constructed. A mutant clone, denoted Delta3297, was obtained by deleting the nucleotide in position 3297, thus inducing a fram...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-006-0767-2
更新日期：2006-10-01 00:00:00
abstract：:Molecular epidemiology can be used to identify human immunodeficiency virus (HIV) transmission clusters, usually using pol sequence for analysis. In the present study, we explored appropriate parameters to construct a simple network using HIV env and gag sequences instead of pol sequences for constructing a phylogenet...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3485-z
更新日期：2017-11-01 00:00:00
abstract：:Seven-day-old embryonated hen eggs were infected with African Horse Sickness virus by the yolk sac and intravenous routes. Virus reached a high titre in the blood of infected embryos. Culicoides variipennis midges which took a blood meal from infected eggs became infected with virus, and after 7 days at 26 degrees - 2...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01347975
更新日期：1975-01-01 00:00:00
abstract：:The genomes of two neurovirulent strains of poliovirus type 3, wild type P3/Leon/37 and a vaccine revertant P3/119/70, have been cloned in E. coli. The cDNA: RNA hybrid method used was efficient and may have wide applicability for cDNA cloning. Overlapping clones spanning the entire genome were obtained for each strai...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309297
更新日期：1984-01-01 00:00:00
abstract：:PCR products representing c. 550 3' terminal bases of Beet soil-borne pomovirus (BSBV) RNA 3 were compared for sources of this virus from all major sugarbeet-growing areas in Germany. In none of these areas conspicious symptoms could be attributed to the presence of BSBV. Single strand conformation polymorphism analys...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070117
更新日期：2000-01-01 00:00:00
abstract：:Rabies is one of the most important zoonotic diseases and is caused by several rabies virus (RABV) variants. These variants can exhibit differences in neurovirulence, and few studies have attempted to evaluate the neuroinvasiveness of variants derived from vampire bats and wild carnivores. The aim of this study was to...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3530-y
更新日期：2017-12-01 00:00:00
abstract：:Seven isolates of West Nile virus and an additional isolate tentatively identified at the outset as West Nile virus, were compared by Casals' modified haemagglutination inhibition technique. Two of the isolates were nearly identical; a second set of five isolates cross-reacted among themselves within a wide range and ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01315279
更新日期：1978-01-01 00:00:00
abstract：:Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) polyhedrin gene from natural mutant strain B, B2 and P was cloned and the amino acid sequence was compared with that of wild-type virus (strain H). Chimeric polyhedrin genes (8 types) containing only one site of mutation within the amino acid sequence were also constr...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050283
更新日期：1998-01-01 00:00:00
abstract：:IG 1, a temperate phage of Bacillus subtilis, is strongly induced from its lysogens by 6-(p-hydroxyphenylazo)-uracil, an azopyrimidine known as selectively inhibiting the B. subtilis DNA polymerase III. IG 1 phages originated either by induction or infection multiply, abundantly, in the presence of that azopyrimidine,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01316671
更新日期：1990-01-01 00:00:00
abstract：:The complete genome of a novel mycovirus, Puccinia striiformis mitovirus 1 (PsMV1), derived from the wheat stripe rust fungus Puccinia striiformis strain SCSN-10, was sequenced and analyzed. The full-length cDNA sequence is 2496 bp in length with a predicted AU content of 57.65% in the genomic RNA. Sequence analysis i...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-018-04134-4
更新日期：2019-03-01 00:00:00
abstract：:The gene encoding the capsid protein of a genogroup I Norwalk-like virus (NLV) (Hu/NLV/Stav/95/Nor) was cloned and expressed in insect cells using a baculovirus vector. The His-tagged recombinant capsid protein (rStav) was antigenic and immunogenic, showed an apparent molecular weight of approximately 68 kD in protein...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050665
更新日期：2000-01-01 00:00:00
abstract：:A replication-defective, recombinant Sindbis virus vector was utilized in a novel immunization strategy to induce humoral and cellular responses against hepatitis C virus (HCV). The recombinant vector, pVaXJ-E1E2, expressing the gene for HCV glycoproteins E2 and E1, was constructed by inserting the E1E2 gene into the ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1564-8
更新日期：2013-05-01 00:00:00
abstract：:The biological function of entomopoxvirus (EPV) spindles (inclusion bodies that lack virions), has not been elucidated. We characterized the function of EPV spindles in the cupreous chafer, Anomala cuprea (Coleoptera: Scarabaeidae). Spheroids or spheroids mixed with spindles of Anomala cuprea EPV were administered per...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050070103
更新日期：2000-01-01 00:00:00
abstract：:In order to determine the prevalence of multiple infections with hepatitis viruses in chronic HBsAg carriers in Naples, to assess the interaction between HBV, HDV and HCV infections and to evaluate the influence of multiple virus hepatitis infections on the clinical presentation, we studied 198 HBsAg chronic carriers ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050090
更新日期：1997-01-01 00:00:00
abstract：:R 61,837, a new antirhinovirus compound, was able to protect several susceptible rhinoviruses against inactivation by mild acidification or heat. This observation strengthens the hypothesis that the drug exerts antiviral activity by a direct interaction with the viral protein capsid to stabilize the particle. However,...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311037
更新日期：1989-01-01 00:00:00
abstract：:Synthesis of the Epstein-Barr virus (EBV) associated early antigen (EA) can be induced by a variety of agents in Raji cells, a latently EBV-infected Burkitt lymphoma line. We investigated the role of lipoproteins in this EA induction system. Cell growth was not affected by lipoprotein-deficiency, but EA induction by m...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01313877
更新日期：1989-01-01 00:00:00
abstract：:A wheat-infecting furovirus found in Tompkins County, New York, U.S.A. was identified as a strain of Soil-borne wheat mosaic virus (SBWMV) by means of sequence analyses of portions of its RNA 1 and 2. The nucleotide sequences of several of its genes differed by c. 9 to 12% from those of the corresponding genome region...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050200011
更新日期：2002-03-01 00:00:00
abstract：:We have developed an in vitro transcriptional assay using Escherichia coli RNA polymerase to initiate the replication of peach latent mosaic viroid (PLMVd). Regardless of the polarity of the PLMVd strand used as template, initiation in vitro occurred at the same hairpin structure. These initiation sites correspond to ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050170061
更新日期：2001-01-01 00:00:00
abstract：:A previously undescribed badnavirus was isolated from Zamia fischeri showing symptoms of chlorosis, necrosis, and ringspot. The virus has bacilliform virions 30 nm in diameter and averaging 120 nm in length. The viral genome is 9227 bp in length and contains three open reading frames characteristic of members of the g...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04630-6
更新日期：2020-07-01 00:00:00
abstract：:Senecavirus A (SVA) is associated with vesicular disease in swine and the acute death of neonatal piglets. Here, senecavirus A was isolated from a pig displaying vesicular disease in Northeast China. The virus was designated as SVA/HLJ/CHA/2016 and its full-length nucleotide sequence was determined and analyzed in com...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3480-4
更新日期：2017-10-01 00:00:00
abstract：:Use of Urografin and Conray for the equilibrium centrifugation of viruses is described. These pharmaceuticals, which consist of iodinated arylic compounds, reach densities of 1.6 g/cm3 and have low intrinsic viscosities. Poliovirus, Newcastle disease virus, and lymphocytic choriomeningitis virus were centrifuged to eq...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318245
更新日期：1975-01-01 00:00:00
abstract：:We have prepared monoclonal antibodies to each of the enteric adenoviruses types 40 and 41. Three different hybridoma cell lines were selected which produced antibody found to react by radioimmunoprecipitation with adenovirus (Ad) hexon antigens. One was specific for Ad 40, another for Ad 41, and a third one reacted w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01310718
更新日期：1987-01-01 00:00:00
abstract：:At low pH influenza virus causes membrane fusion. This phenomenon is thought to reflect a part of the infection mechanism of the virus. To obtain more information on the effect of low pH on the virus, the change in morphology of influenza virus particles was studied by electron microscopy. Further, the degradation of ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01311162
更新日期：1984-01-01 00:00:00
abstract：:Enzootic bovine leukosis (EBL) is a malignant B-cell lymphoma of cattle caused by infection with bovine leukemia virus (BLV). It is defined by clonal and neoplastic expansion of BLV-infected B cells. Currently, multiple examinations are able to comprehensively diagnose this condition. Inverse polymerase chain reaction...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-020-04945-4
更新日期：2021-01-24 00:00:00
abstract：:We previously reported the isolation of South River virus (SORV) from a pool of mosquitoes collected in the Yucatan Peninsula of Mexico (Farfan-Ale et al. in Vector Borne Zoonotic Dis 10:777-783, 5). The isolate (designated SORV-252) was identified as SORV after a 197-nucleotide region of its small RNA genome segment ...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-012-1280-4
更新日期：2012-06-01 00:00:00
abstract：:Stem-pitting (SP) is the main type of citrus tristeza virus (CTV) that causes severe damage to citrus trees, especially those of sweet orange, in Hunan province, China. Understanding the local CTV population structure should provide clues for effective mild strain cross-protection (MSCP) of the SP strain of CTV. In th...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-016-3089-z
更新日期：2017-02-01 00:00:00
abstract：:Arginine is necessary for the development of the cytopathogenic effect of human cytomegalovirus in human embryonic fibroblasts. It is also required, though in greater concentrations, for the production of infective virions, the requirement being at an early stage of replication. Inhibitor studies suggested that this b...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01318146
更新日期：1975-01-01 00:00:00
