Molecular characterization of a distinct begomovirus infecting tobacco in Yunnan, China.

Abstract:

:Virus isolate Y3V, obtained from tobacco showing leaf curl symptoms in Yunnan, China, had particles with the size and morphology typical of geminiviruses. In reactions with a set of monoclonal antibodies raised against begomoviruses, Y3V was readily differentiated from two previously studied Chinese Begomovirus isolates. The complete nucleotide sequence of a DNA-A-like molecule of Y3V was determined; it comprises 2744 nucleotides and has a typical Begomovirus genome organization. When compared with the DNA-A sequences of other begomoviruses, the total DNA-A of Y3V was most closely related to that of Ageratum yellow vein virus (AYVV) (85% sequence identity), but the Y3V intergenic region differed greatly from those of the other sequences (maximum 70% identity). In contrast, the deduced coat protein of Y3V is most like that of Tomato yellow leaf curl Thailand virus-[1] (TYLCTHV-[1]) (92% amino acid sequence identity). The molecular data show that the Yunnan isolate of Tobacco leaf curl virus is a distinct Begomovirus species, for which the name Tobacco leaf curl Yunnan virus (TLCYnV) is proposed.

journal_name

Arch Virol

journal_title

Archives of virology

authors

Zhou XP,Xie Y,Zhang ZK

doi

10.1007/s007050170081

keywords:

subject

Has Abstract

pub_date

2001-08-01 00:00:00

pages

1599-606

issue

8

eissn

0304-8608

issn

1432-8798

journal_volume

146

pub_type

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